Single-molecule epiallelic profiling of DNA derived from routinely collected Pap specimens for noninvasive detection of ovarian cancer

Christine M. O’Keefe; Yang Zhao; Leslie M. Cope; Chih-Ming Ho; Amanda N. Fader; Rebecca Stone; James S. Ferris; Anna Beavis; Kimberly Levinson; Stephanie Wethington; Tian-Li Wang; Thomas R. Pisanic; Ie-Ming Shih; Tza-Huei Wang

doi:10.1002/ctm2.1778

Clinical and Translational Medicine ›› 2024, Vol. 14 ›› Issue (8) : e1778 DOI: 10.1002/ctm2.1778

RESEARCH ARTICLE

Single-molecule epiallelic profiling of DNA derived from routinely collected Pap specimens for noninvasive detection of ovarian cancer

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Abstract

	•We present a microfluidic platform for detection and analysis of rare, heterogeneously methylated DNA within Pap specimens towards detection of ovarian cancer.
	•The platform achieves high sensitivity (fractions <0.00005%) at a suitably low cost (~$25) for routine screening applications.
	•Furthermore, it provides molecule-by-molecule quantitative analysis to facilitate further study on the effect of heterogeneous methylation on cancer development.

Keywords

digital melt / DNA methylation / microfluidics / ovarian cancer / Pap smear

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Christine M. O’Keefe, Yang Zhao, Leslie M. Cope, Chih-Ming Ho, Amanda N. Fader, Rebecca Stone, James S. Ferris, Anna Beavis, Kimberly Levinson, Stephanie Wethington, Tian-Li Wang, Thomas R. Pisanic, Ie-Ming Shih, Tza-Huei Wang. Single-molecule epiallelic profiling of DNA derived from routinely collected Pap specimens for noninvasive detection of ovarian cancer. Clinical and Translational Medicine, 2024, 14(8): e1778 DOI:10.1002/ctm2.1778

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2024 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.