The rise of one-pot nucleic acid detection using CRISPR

Shuobo Shi , Qingqing Shang , Wenzhe Si , Jinyu Fu , Yaru Li

Clinical and Translational Discovery ›› 2025, Vol. 5 ›› Issue (5) : e70091

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Clinical and Translational Discovery ›› 2025, Vol. 5 ›› Issue (5) :e70091 DOI: 10.1002/ctd2.70091
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The rise of one-pot nucleic acid detection using CRISPR

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Shuobo Shi, Qingqing Shang, Wenzhe Si, Jinyu Fu, Yaru Li. The rise of one-pot nucleic acid detection using CRISPR. Clinical and Translational Discovery, 2025, 5(5): e70091 DOI:10.1002/ctd2.70091

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Jinyu Fu, Li J, Chen J, et al. Ultra-specific nucleic acid testing by target activated nucleases. Crit Rev Biotechnol. 2021; 42: 1061-1078.

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Fu J, Mo R, Li Z, et al. An extraction-free one-pot assay for rapid detection of Klebsiella pneumoniae by combining RPA and CRISPR/Cas12a. Biosens Bioelectron. 2025; 267:116740.

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Ding X, Yin K, Li Z, et al. Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay. Nat Commun. 2020; 11: 4711.

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Hu M, Qiu Z, Bi Z, Tian T, Jiang Y, Zhou X. Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics. Proc Natl Acad Sci USA. 2022; 119:e2202034119.

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2025 The Author(s). Clinical and Translational Discovery published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.

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