High-resolution single-cell transcriptomic survey of cardiomyocytes from patients with hypertrophic cardiomyopathy
Received date: 02 Jun 2023
Revised date: 28 Aug 2023
Accepted date: 15 Sep 2023
Copyright
Hypertrophic cardiomyopathy (HCM) is a common inherited cardiovascular disease, which can cause heart failure and lead to death. In this study, we performed high-resolution single-cell RNA-sequencing of 2115 individual cardiomyocytes obtained from HCM patients and normal controls. Signature up- and down-regulated genes in HCM were identified by integrative analysis across 37 patients and 41 controls from our data and published human single-cell and single-nucleus RNA-seq datasets, which were further classified into gene modules by single-cell co-expression analysis. Using our high-resolution dataset, we also investigated the heterogeneity among individual cardiomyocytes and revealed five distinct clusters within HCM cardiomyocytes. Interestingly, we showed that some extracellular matrix (ECM) genes were up-regulated in the HCM cardiomyocytes, suggesting that they play a role in cardiac remodelling. Taken together, our study comprehensively profiled the transcriptomic programs of HCM cardiomyocytes and provided insights into molecular mechanisms underlying the pathogenesis of HCM.
Jiansen Lu , Jie Ren , Jie Liu , Minjie Lu , Yueli Cui , Yuhan Liao , Yuan Zhou , Yun Gao , Fuchou Tang , Jizheng Wang , Shuiyun Wang , Lu Wen , Lei Song . High-resolution single-cell transcriptomic survey of cardiomyocytes from patients with hypertrophic cardiomyopathy[J]. Cell Proliferation, 2024 , 57(3) : e13557 . DOI: 10.1111/cpr.13557
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