Requirements for human natural killer cells

Shuaishuai Niu; Chengxiang Xia; Dehao Huang; Lei Wang; Hongbo Hu; Shuyang Yu; Ning Wu; Zhongjun Dong; Jiaxi Zhou; Jun Wu; Junying Yu; Ying Zhang; Changlin Wang; Boqiang Fu; Jiani Cao; Lingmin Liang; Lingxue Xu; Ling Chen; Qi Zhou; Aijin Ma; Tongbiao Zhao; Jie Hao; Jinyong Wang

doi:10.1111/cpr.13588

Cell Proliferation ›› 2024, Vol. 57 ›› Issue (5) : e13588 DOI: 10.1111/cpr.13588

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Requirements for human natural killer cells

Shuaishuai Niu ¹^,²^,³^,⁴^,⁵
, Chengxiang Xia ⁵
, Dehao Huang ²
, Lei Wang ¹^,²^,³^,⁴^,⁵
, Hongbo Hu ⁶
, Shuyang Yu ⁷
, Ning Wu ⁸
, Zhongjun Dong ⁹
, Jiaxi Zhou ³^,⁴^,¹⁰
, Jun Wu ¹^,⁵^,¹¹
, Junying Yu ³^,⁴^,¹²
, Ying Zhang ¹²
, Changlin Wang ³^,⁴^,¹³
, Boqiang Fu ³^,⁴^,¹⁴
, Jiani Cao ¹^,²^,³^,⁴^,⁵^,¹¹
, Lingmin Liang ¹^,²^,³^,⁴^,¹¹^,¹⁵
, Lingxue Xu ¹^,¹¹^,¹⁵
, Ling Chen ¹^,¹¹^,¹⁵
, Qi Zhou ¹^,²^,³^,⁴^,⁵
, Aijin Ma ³^,⁴^,¹⁶^,^†
, Tongbiao Zhao ¹^,²^,³^,⁴^,⁵^,¹¹^,^†
, Jie Hao ¹^,²^,³^,⁴^,⁵^,¹¹^,^†
, Jinyong Wang ²^,⁵^,¹¹^,^†

Author information +

¹. National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, China

². State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China

³. Standards Committee, Chinese Society for Stem Cell Research, Chinese Society for Cell Biology, Shanghai, China

⁴. Chinese Society for Stem Cell Research, Shanghai, China

⁵. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China

⁶. Center for Hematology and Immunology, Cancer Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China

⁷. State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, China

⁸. Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

⁹. State Key Laboratory of Membrane Biology, School of Medicine and Institute for Immunology, Tsinghua University, Beijing, China

¹⁰. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China

¹¹. University of Chinese Academy of Sciences, Beijing, China

¹². Nuwacell Biotechnology Co., Ltd, Hefei City, Anhui Province, China

¹³. China National Institute of Standardization, China

¹⁴. National Institute of Metrology, Beijing, China

¹⁵. Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China

¹⁶. Beijing Technology and Business University, Beijing, China

maaj@btbu.edu.cn

tbzhao@ioz.ac.cn

haojie@ioz.ac.cn

wangjinyong@ioz.ac.cn

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Abstract

‘Requirements for Human Natural Killer Cells’ is the latest set of guidelines on human NK cells in China, jointly drafted and agreed upon by experts from the Standards Committee of Chinese Society for Cell Biology. This standard specifies requirements for the human natural killer (NK) cells, including the technical requirements, test methods, test regulations, instructions for use, labeling requirements, packaging requirements, storage and transportation requirements, and waste disposal requirements of NK cells. This standard is applicable for the quality control of NK cells, derived from human tissues, or differentiated/transdifferentiated from stem cells. It was originally released by the Chinese Society for Cell Biology on 30 August, 2022. We hope that the publication of these guidelines will promote institutional establishment, acceptance, and execution of proper protocols and accelerate the international standardization of human NK cells for applications.

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Shuaishuai Niu,Chengxiang Xia,Dehao Huang,Lei Wang,Hongbo Hu,Shuyang Yu,Ning Wu,Zhongjun Dong,Jiaxi Zhou,Jun Wu,Junying Yu,Ying Zhang,Changlin Wang,Boqiang Fu,Jiani Cao,Lingmin Liang,Lingxue Xu,Ling Chen,Qi Zhou,Aijin Ma,Tongbiao Zhao,Jie Hao,Jinyong Wang. Requirements for human natural killer cells. Cell Proliferation, 2024, 57(5): e13588 DOI:10.1111/cpr.13588