Identification of missing CYP450 enzymes involved in paclitaxel biosynthesis and heterologous reconstitution of baccatin III

Keywords

Paclitaxel biosynthesis / Baccatin III / Cytochrome P450 / Taxane oxetanase 1 / Heterologous reconstitution

References

[1] Luo ZW, Yin FC, Wang XB, et al. Progress in approved drugs from natural product resources[J]. Chin J Nat Med, 2024, 22(3): 195-211.

[2] Wheeler NC, Jech K, Masters S, et al. Effects of genetic, epigenetic, and environmental factors on taxol content in Taxus brevifolia and related species[J]. J Nat Prod, 1992, 55(4): 432-440.

[3] Zhou XW, Zhu HF, Liu L, et al. A review: recent advances and future prospects of taxol-producing endophytic fungi[J]. Appl Microbiol Biotechnol, 2010, 86(6): 1707-1717.

[4] Xiong XY, Gou JB, Liao QG, et al. The Taxus genome provides insights into paclitaxel biosynthesis[J]. Nat Plants, 2021, 7(8): 1026-1036.

[5] Jiang B, Gao L, Wang HJ, et al. Characterization and heterologous reconstitution ofTaxus biosynthetic enzymes leading to baccatin III[J]. Science, 2024, 383(6683): 622-629.

[6] Wang MM, Cornett B, Nettles J, et al. The oxetane ring in taxol[J]. J Org Chem, 2000, 65(4): 1059-1068.

[7] Croteau R, Ketchum RE, Long RM, et al. Taxol biosynthesis and molecular genetics[J]. Phytochem Rev, 2006, 5(1): 75-97.

[8] Wang T, Li LY, Zhuang WB, et al. Recent research progress in taxol biosynthetic pathway and acylation reactions mediated by taxus acyltransferases[J]. Molecules, 2021, 26(10): 2855.

[9] Zhang YJ, Wiese L, Fang H, et al. Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis[J]. Mol Plant, 2023, 16(12): 1951-1961.