Real-time analysis of nanoscale dynamics in membrane protein insertion via single-molecule imaging

Chenguang Yang; Dongfei Ma; Shuxin Hu; Ming Li; Ying Lu

doi:10.52601/bpr.2024.240024

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Biophysics Reports ›› 2024, Vol. 10 ›› Issue (6) : 369-376. DOI: 10.52601/bpr.2024.240024

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Real-time analysis of nanoscale dynamics in membrane protein insertion via single-molecule imaging

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Abstract

Membrane proteins often need to be inserted into or attached to the cell membrane to perform their functions. Understanding their transmembrane topology and conformational dynamics during insertion is crucial for elucidating their roles. However, it remains challenging to monitor nanoscale changes in the insertion depth of individual proteins in membranes. Here, we introduce two single-molecule imaging methods, SIFA and LipoFRET, designed for in vitro observation of the nanoscale architecture of membrane proteins within membranes. These methods have demonstrated their efficacy in studying biomolecules interacting with bio-membranes with sub-nanometer precision.

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Membrane protein / Insert / Real time / Single molecule / Nanoscale

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Chenguang Yang, Dongfei Ma, Shuxin Hu, Ming Li, Ying Lu. Real-time analysis of nanoscale dynamics in membrane protein insertion via single-molecule imaging. Biophysics Reports, 2024, 10(6): 369‒376 https://doi.org/10.52601/bpr.2024.240024

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Acknowledgements

This work was supported by the Guangdong Basic and Applied Basic Research Foundation (2020A1515110102), the National Natural Science Foundation of China (12022409 and T2221001) and the CAS Key Research Program of Frontier Sciences (ZDBS-LY-SLH015).