A simple yet effective H₂S-activated fluorogenic probe for precise imaging of hepatitis and arthritis in situ

Abstract

Hepatitis and arthritis are prevalent inflammatory diseases, and the utilization of fluorogenic probes incorporating hydrogen sulfide (H₂S) as a crucial mediator of inflammation presents significant opportunities for early detection. However, the poor in vivo biodistribution and limited targeted efficacy of molecule probes for inflammation imaging severely impede their ability to differentiate the extent of inflammation and provide real-time monitoring of inflammatory levels. Therefore, we developed a highly efficient H₂S-activated near-infrared (NIR) fluorogenic probe (hCy-DNP) for real-time tracking and capturing fluctuations in H₂S levels within inflammatory lesions. hCy-DNP demonstrates an exceptionally sensitive fluorescence response to H₂S expression, enabling specific differentiation between various levels of lipopolysaccharide (LPS) -stimulated early hepatitis models in situ, while also facilitating visual monitoring for diagnosis and efficacy evaluation of arthritis. Therefore, hCy-DNP offers an innovative tool for exploring early diagnosis and evaluating treatment effectiveness across diverse inflammatory diseases.

Keywords

arthritis / fluorescence imaging / fluorogenic probe / H₂S / hepatitis

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2024 The Authors. BMEMat published by John Wiley & Sons Australia, Ltd on behalf of Shandong University.