Self-healing approach for micro-defects in 3D printed concrete: Microbial community

Abstract

Interlayer gaps and shrinkage cracks have significant impact to negate the mechanical properties, especially durability, of 3D printed concrete, which registers a lasting challenge hindering practical engineering application of 3D concrete printing technology. To this gap, this study translates the microbial-induced calcium carbonate precipitation (MICP) for casted concrete into a novel microbial community self-healing agent with high calcium carbonate deposition performance for 3D printed concrete. Under microaerobic conditions, the mineralization performance of the microbial community is 3.63 times that of commonly used self-healing bacteria B. pasteurii. The microbial community structure demonstrated that Pseudogracilibacillus is the dominant genus in both aerobic and microaerobic conditions. Under micro-oxygen conditions, relative abundance ofunclassified_Bacillaceae_2 increases sharply to 32.08%–57.11%, registering the dominant genus to jointly promote mineralization. Addition of a microbial community self-healing agent will increase the flowability and setting time of 3D-printed concrete, and thus improving its cracking resistance. The total porosity, including interlayer gaps, is measured to be 0.72% at 1 day curing, which is one-third that of the control group. Compared to the counterparts in control group without the microbial community, the air voids and microcracks, mesopores, and macropores, decrease by 40%, 14%, and 72%, respectively. Among all the different crack depths observed, for cracks with an opening width of approximately 1 millimeter, the repairing area reachs 100% within 7 days of curing. Given the efficient metabolism of facultative bacteria under microaerobic conditions, the average depth of crack repairing is 30 mm, and the maximum depth can reach even 38 mm. It can be stated this research promises to resolve efficiently the long standing micro-defect problem of 3D-printed concrete.

Keywords

3D-printed concrete / Microbial community / Micro-defect / Self-healing

CRediT authorship contribution statement

Guowei Ma: Supervision, Resources. Li Wang: Resources. Yimiao Huang: Supervision. Yiran Liu: Data curation. Jiaxing Li: Writing – original draft, Data curation. Ruochen Zhang: Writing – review & editing, Writing – original draft, Project administration, Conceptualization.

Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgement

This research is financially supported by the National Natural Science Foundation of China [Grant Nos.: 52108204 and 52293434] and Natural Science Foundation of Hebei Province, China [Grant No.: E2021202088]. Special thanks to Professor Fang Wang for her guidance on this research work.

References

[1] Achal, V., Mukherjee, A., Basu, P. C., & Reddy, M. S. (2009). Strain improvement of sporosarcina pasteurii for enhanced urease and calcite production. Journal of Industrial Microbiology and Biotechnology, 36, 981-988. https://doi.org/10.1007/s10295-009-0578-z

[2] Algaifi, H. A., Bakar, S. A., Sam, A. R. M., Ismail, M., Abidin, A. R. Z., Shahir, S., & Altowayti, W. A. H. (2020). Insight into the role of microbial calcium carbonate and the factors involved in self-healing concrete. Construction and Building Materials, 254, Article 119258. https://doi.org/10.1016/j.conbuildmat.2020.119258

[3] Altheoy, F., Zaid, O., Ahmed, B., & Elhadi, K. M. (2024). Impact of double hooked steel fibers and nano-kaolin clay on fresh properties of 3D-Printable Ultra-High-Performance Fiber-Reinforced concrete. Journal of Building Engineering, 97, Article 110917. https://doi.org/10.1016/j.jobe.2024.110917

[4] ASTM (2016). Standard test method for compressive strength of hydraulic cement mortars. ASTM C, 109 .

[5] Chen, H., Qian, C., & Huang, H. (2016). Self-healing cementitious materials based on bacteria and nutrients immobilized respectively.Construction and Building Materials, 126, 297-303. https://doi.org/10.1016/j.conbuildmat.2016.09.023

[6] Chen, Y., Xia, K., Jia, Z., Gao, Y., Zhang, Z., & Zhang, Y. (2024). Extending applicability of 3D-printable geopolymer to large-scale printing scenario via combination of sodium carbonate and nano-silica. Cement and Concrete Composites, 145, Article 105322. https://doi.org/10.1016/j.cemconcomp.2023.105322

[7] Chindasiriphan, P., Subwilai, N., Intarasoontron, J., Nuaklong, P., Jongvivatsakul, P., Chompoorat, T., Pungrasmi, W., & Likitlersuang, S. (2024). Synergistic effects of microencapsulated bacterial spores and superabsorbent polymer on self-healing performance in mortar. Construction and Building Materials, 414, Article 135005. https://doi.org/10.1016/j.conbuildmat.2024.135005

[8] Choi, S. G., Wang, K. J., Wen, Z. Y., & Chu, J. (2017). Mortar crack repair using microbial induced calcite precipitation method. Cement and Concrete Composites, 83, 209-221. https://doi.org/10.1016/j.cemconcomp.2017.07.013

[9] Ding, T., Xiao, J., & Mechtcherine, V. (2023). Microstructure and mechanical properties of interlayer regions in extrusion-based 3D printed concrete: A critical review. Cement and Concrete Composites, 141, Article 105154. https://doi.org/10.1016/j.cemconcomp.2023.105154

[10] Dinges, M. M., Orwin, P. M., & Schlievert, P. M. (2000). Exotoxins of staphylococcus aureus. Clinical Microbiology Reviews, 13, 16-34. https://doi.org/10.1128/cmr.13.1.16

[11] Dubey, A. A., Ravi, K., Mukherjee, A., Sahoo, L., Moses, A. A., & Dhami, N. K. (2021). Biocementation mediated by native microbes from Brahmaputra riverbank for mitigation of soil erodibility. Scientific Reports, 11, 15250. https://doi.org/10.1038/541598-021-94614-6

[12] Ersan, Y. C., Da Silva, F. B., Boon, N., Verstraete, W., & De Belie, N. (2015). Screening of bacteria and concrete compatible protection materials. Construction and Building Materials, 88, 196-203. https://doi.org/10.1016/j.conbuildmat.2015.04.027

[13] Fang, C., & Achal, V. (2024). Enhanced steel corrosion inhibition through microbially induced carbonate precipitation with facultative anaerobic denitrifying bacterium. Case Studies in Construction Materials, 21, Article e03607. https://doi.org/10.1016/j.cscm.2024.e03607

[14] Fang, C., & Achal, V. (2025). Enhancing engineering properties of cement mortars through microbial self-healing and community analysis. Construction and Building Materials, 462, Article 139934. https://doi.org/10.1016/j.conbuildmat.2025.139934

[15] Feng, J., Chen, B., Sun, W., & Wang, Y. (2021). Microbial induced calcium carbonate precipitation study using bacillus subtilis with application to self-healing concrete preparation and characterization. Construction and Building Materials, 280, Article 122460. https://doi.org/10.1016/j.conbuildmat.2021.122460

[16] Fu, Q., Liu, M., Liu, S., Lu, L., Fang, N., Chen, J., Shama, S., & Wang, J. (2023). Growth and mineralization characteristics of bacillus subtilis isolated from marine aquaculture wastewater and its application in coastal self-healing concrete. Materials Today Communications, 35, Article 105654. https://doi.org/10.1016/j.mtcomm.2023.105654

[17] Harden, V. P., & Harris, J. O. V. (1953). The isoelectric point of bacterial cells. Journal of Bacteriology , (2), 198-202. https://doi.org/10.1128/jb.65.2.198-202.1953

[18] Hong, Y., & Brown, D. G. Y. (2008). Electrostatic behavior of the charge-regulated bacterial cell surface. Langmuir, 9, 5003-5009. https://doi.org/10.1021/la703564q

[19] Hosseini, E., Zakertabrizi, M., Korayem, A. H., & Xu, G. (2019). A novel method to enhance the interlayer bonding of 3D printing concrete: An experimental and computational investigation. Cement and Concrete Composites, 99, 112-119. https://doi.org/10.1016/j.cemconcomp.2019.03.008

[20] Jiang, L., Jia, G., Jiang, C., & Li, Z. (2020). Sugar-coated expanded perlite as a bacterial carrier for crack-healing concrete applications. Construction and Building Materials, 232, Article 117222. https://doi.org/10.1016/j.conbuildmat.2019.117222

[21] Joshi, S., Goyal, S., & Reddy, M. S. (2021). Bio-consolidation of cracks with Fly ash amended biogrouting in concrete structures. Construction and Building Materials, 300, Article 124044. https://doi.org/10.1016/j.conbuildmat.2021.124044

[22] Keykha, H. A., Huat, B. B. K., Asadi, A., Zareian, M., & Kawasaki, S. (2015). Electrokinetic properties of pasteurii and aquimarina bacteria. Environmental Geotechnics, 3, 181-188. https://doi.org/10.1680/envgeo.13.00072

[23] Lan, X., Zeng, X., Zhu, H., Long, G., & Xie, Y. (2022). Experimental investigation on fractal characteristics of pores in air-entrained concrete at low atmospheric pressure. Cement and Concrete Composites, 130, Article 104509. https://doi.org/10.1016/j.cemconcomp.2022.104509

[24] Lemos, L. N., Fulthorpe, R. R., Triplett, E. W., & Roesch, L. F. (2011). Rethinking microbial diversity analysis in the high throughput sequencing era. Journal of Microbiological Methods, 86, 42-51. https://doi.org/10.1016/j.mimet.2011.03.014

[25] Li, X., He, X., Ren, K., Dong, H., & Lian, B. (2023). Mechanisms of carbonate precipitation induced by two model bacteria. Chem. Geol, 628 , Article 121461. https://doi.org/10.1016/j.chemgeo.2023.121461

[26] Liu, C., Zhang, R., Liu, H., He, C., Wang, Y., Wu, Y., Liu, S., Song, L., & Zuo, F. (2022). Analysis of the mechanical performance and damage mechanism for 3D printed concrete based on pore structure. Construction and Building Materials, 314, Article 125572. https://doi.org/10.1016/j.conbuildmat.2021.125572

[27] Luo, M., & Qian, C. (2016). Influences of bacteria-based self-healing agents on cementitious materials hydration kinetics and compressive strength. Construction and Building Materials, 121, 659-663. https://doi.org/10.1016/j.conbuildmat.2016.06.075

[28] Ma, G., Ruhan, A., Xie, P., Pan, Z., Wang, L., & Hower, J. C. (2022). 3D-printable aerogel-incorporated concrete: Anisotropy influence on physical, mechanical, and thermal insulation properties. Construction and Building Materials, 323, Article 126551. https://doi.org/10.1016/j.cemconcomp.2024.105811

[29] Mohan, M. K., Rahul, A. V., Stappen, J. F. V., Cnudde, V., Schutter, G. D., & Tittelboom, K. V. (2023). Assessment of pore structure characteristics and tortuosity of 3D printed concrete using Mercury intrusion porosimetry and X-ray tomography. Cement and Concrete Composites, 140, Article 105104. https://doi.org/10.1016/j.cemconcomp.2023.105104

[30] Nerella, V. N., Krause, M., & Mechtcherine, V. (2020). Direct printing test for buildability of 3D-printable concrete considering economic viability. Automat. Constr, 109, Article 102986. https://doi.org/10.1016/j.autcon.2019.102986

[31] Pei, R., Liu, J., Wang, S., & Yang, M. (2013). Use of bacterial cell walls to improve the mechanical performance of concrete. Cement and Concrete Composites, 39, 122-130. https://doi.org/10.1016/j.cemconcomp.2013.03.024

[32] Putten, J. V. D., Volder, M. D., den Heede, P. V., Schutter, G. D., & Tittelboom, K. V. (2020). 3D printing of concrete: The influence on chloride penetration. RILEM International Conference on Concrete and Digital Fabrication . Cham: Springer 500-507. https://doi.org/10.1007/978-3-030-49916-7_51

[33] Qian, C., Ren, L., Xue, B., & Cao, T. (2016). Bio-mineralization on cement-based materials consuming CO₂ from atmosphere. Construction and Building Materials, 106, 126-132. https://doi.org/10.1016/j.conbuildmat.2015.10.105

[34] Qian, C., Zhang, Y., & Xie, Y. (2021a). Effect of ion concentration in crack zone on healing degree of microbial self-healing concrete. Construction and Building Materials, 286. https://doi.org/10.1016/j.conbuildmat.2021.122969

[35] Qian, C., Zheng, T., Zhang, X., & Su, Y. (2021b). Application of microbial self-healing concrete: Case study. Construction and Building Materials, 290, Article 123226. https://doi.org/10.1016/j.conbuildmat.2021.123226

[36] Saulat, H., Cao, M., Khan, M. M., Khan, M., Khan, M. M., & Rehman, A. (2020). Preparation and applications of calcium carbonate whisker with a special focus on construction materials. Construction and Building Materials, 236, Article 117613. https://doi.org/10.1016/j.conbuildmat.2019.117613

[37] Su, Y., Feng, J., Jin, P., & Qian, C. (2019). Influence of bacterial self-healing agent on early age performance of cement-based materials. Construction and Building Materials, 218, 224-234. https://doi.org/10.1016/j.conbuildmat.2019.05.077

[38] Su, Y., Liu, S., Jin, P., & Zhan, Q. (2023). Repairing performance and bottleneck analysis of concrete with encapsulated oxygen-dependent microbial agent: Simulation-Aided characterization. Construction and Building Materials, 408, Article 113764. https://doi.org/10.1016/j.conbuildmat.2023.133764

[39] Su, Y., Zheng, T., & Qian, C. (2021). Application potential of bacillus megaterium encapsulated by low alkaline sulphoaluminate cement in self-healing concrete. Construction and Building Materials, 273, Article 121740. https://doi.org/10.1016/j.conbuildmat.2020.121740

[40] Sun, H., Wang, X., Xu, J., Chen, Q., & Zhu, H. (2024). Effect of seawater salinity on ureolysis-based microbial self-healing of concrete cracks. Construction and Building Materials, 453, Article 138993. https://doi.org/10.1016/j.conbuildmat.2024.138993

[41] Tran, N. P., Nguyen, T. N., & Ngo, T. D. (2022). The role of organic polymer modifiers in cementitious systems towards durable and resilient infrastructures: A systematic review. Construction and Building Materials, 360, Article 129562. https://doi.org/10.1016/j.conbuildmat.2022.129562

[42] Tripathi, A., Nair, S. A. O., & Neithalath, N. (2022). A comprehensive analysis of buildability of 3D-printed concrete and the use of bi-linear stress-strain criterion-based failure curves towards their prediction. Cement and Concrete Composites, 128, Article 104424. https://doi.org/10.1016/j.cemconcomp.2022.104424

[43] Tugluca, M. S., Ersan, Y.Ç., & Şahmaran, M. (2024). Variation of microbial self-healing performance of cementitious composites with their biogranule content. Cement and Concrete Composites, 152, Article 105616. https://doi.org/10.1016/j.cemconcomp.2024.105616

[44] Vaskevicius, L., Malunavicius, V., Jankunec, M., Lastauskiene, E., Martynas, T., Lina, M., Andriuss, M., & Renata, G. (2023). Insights in MICP dynamics in urease-positive staphylococcus sp. H6 and sporosarcina pasteurii bacterium. Environ. Res. 234, Article 116588. https://doi.org/10.1016/j.envres.2023.116588

[45] Wang, L., Jiang, H., Li, Z., & Ma, G. (2020b). Mechanical behaviors of 3D printed lightweight concrete structure with hollow section. Archives of Civil and Mechanical Engineering, 16. https://doi.org/10.1007/s43452-020-00017-1

[46] Wang, L., Tian, Z., Ma, G., & Zhang, M. (2020a). Interlayer bonding improvement of 3D printed concrete with polymer modified mortar: Experiments and molecular dynamics studies. Cement and Concrete Composites, 110, Article 103571. https://doi.org/10.1016/j.cemconcomp.2020.103571

[47] Wang, X., Xu, J., Wang, Z., & Yao, W. (2022). Use of recycled concrete aggregates as carriers for self-healing of concrete cracks by bacteria with high urease activity. Construction and Building Materials, 337, Article 127581. https://doi.org/10.1016/j.conbuildmat.2022.127581

[48] Wi, K., Wang, K., Taylor, P. C., Laflamme, S., Sritharan, S., & Qin, H. (2021). Properties and microstructure of extrusion-based 3D printing mortar containing a highly flowable, rapid set grout. Cement and Concrete Composites, 124, Article 104243. https://doi.org/10.1016/j.cemconcomp.2021.104243

[49] Wong, P. Y., Mal, J., Sandak, A., Luo, L., Jian, J., & Pradhan, N. (2024). Advances in microbial self-healing concrete: A critical review of mechanisms, developments, and future directions. Science of the Total Environment, 947, 74553. https://doi.org/10.1016/j.scitotenv.2024.174553

[50] Yoshioka, K., Tazawa, E., Kawai, K., & Enohata, T. (2002). Adsorption characteristics of superplasticizers on cement component minerals. Cement and Concrete Composites, 32, 1507-1513. https://doi.org/10.1016/S0008-8846(02)00782-2

[51] Zaid, O., & Ouni, M. H. E. (2024). Advancements in 3D printing of cementitious materials: a review of mineral additives, properties, and systematic developments. Construction and Building Materials, 427, Article 136254. https://doi.org/10.1016/j.conbuildmat.2024.136254

[52] Zhang, G. Z., Li, S., Li, H. F., Zhang, K., & Cheng, P. F. (2024b). Enhancing self-healing performance of microbial mortar through carbon fiber reinforcement: An experimental analysis. Journal of Building Engineering, 91, Article 109499. https://doi.org/10.1016/j.jobe.2024.109499

[53] Zhang, J., Liu, Y., Feng, T., Zhou, M., Zhao, L., Zhou, A., & Li, Z. (2017c). Immobilizing bacteria in expanded perlite for the crack self-healing in concrete. Construction and Building Materials, 148, 610-617. https://doi.org/10.1016/j.conbuildmat.2017.05.021

[54] Zhang, J., Mai, B., Cai, T., Luo, J., Wu, W., Liu, B., Han, N., Xing, F., & Deng, X. (2017a). Optimization of a binary concrete crack self-healing system containing bacteria and oxygen. Materials, 10, 116. https://doi.org/10.3390/ma10020116

[55] Zhang, J. H., Shi, X. Z., Guan, W. M., Liu, Y., Xia, X. C., Yuan, J. J., & Chen, X. (2023). Mineralization performance and crystal characteristics of microbial induced carbonate precipitation in lead-zinc tailings under multiple factors. Construction and Building Materials, 403, Article 133081. https://doi.org/10.1016/j.conbuildmat.2023.133081

[56] Zhang, Q., Zhang, X., Zhao, H., & Su, Y. (2024a). A full-depth self-healing strategy for cracks in cement-based materials under marine environment. Construction and Building Materials, 449, Article 138295. https://doi.org/10.1016/j.conbuildmat.2024.138295

[57] Zhang, J., Zhao, C., Zhou, A., Yang, C., Zhao, L., & Li, Z. (2019). Aragonite formation induced by open cultures of microbial consortia to heal cracks in concrete: Insights into healing mechanisms and crystal polymorphs. Construction and Building Materials, 224, 815-822. https://doi.org/10.1016/j.conbuildmat.2019.07.129

[58] Zhang, J., Zhou, A., Liu, Y., Zhao, B., Luan, Y., Wang, S., Yue, X., & Li, Z. (2017b). Microbial network of the carbonate precipitation process induced by microbial consortia and the potential application to crack healing in concrete. Sci. Rep-UK, 7, Article 14600. https://doi.org/10.1038/s41598-017-15177-z

[59] Zhao, C., Toufigh, V., Zhang, J., Liu, Y., Fan, W., He, X., Cao, B., & Xiao, Y. (2023). Enhancing biomineralization process efficiency with trained bacterial strains: A technical perspective. Biogeotechnics, 1, Article 100017. https://doi.org/10.1016/j.bgtech.2023.100017

[60] Zhao, D. D., Williams, J. M., Hou, P. K., Moment, A. J., & Kawashima, S. (2024). Stabilizing mechanisms of metastable vaterite in cement systems. Cement and Concrete Research, 178, Article 107441. https://doi.org/10.1016/j.cemconres.2024.107441

[61] Zheng, T., Su, Y., Qian, C., & Zhou, H. (2020). Low alkali sulpho-aluminate cement encapsulated microbial spores for self-healing cement-based materials. Biochemical Engineering Journal, 163, Article 107756. https://doi.org/10.1016/j.bej.2020.107756