Soil-water retention capacity of expansive soil improved through enzyme induced carbonate precipitation-eggshell powder

Yunlong Liu , Yanyan Xia , Mudassir Mehmood , Lei Wang , Wen Nie , Yingao Zhao , Zhencai Luo

Biogeotechnics ›› 2025, Vol. 3 ›› Issue (3) : 100146

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Biogeotechnics ›› 2025, Vol. 3 ›› Issue (3) :100146 DOI: 10.1016/j.bgtech.2024.100146
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Soil-water retention capacity of expansive soil improved through enzyme induced carbonate precipitation-eggshell powder

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Abstract

Enzyme Induced Carbonate Precipitation (EICP) has been extensively investigated as a promising approach to improve engineering properties of soil, while Eggshell Powder (ESP) is an agricultural waste that effectively fills soil pores. The ESP provides abundant nucleation at sites for the EICP process, further promoting the effective precipitation of calcium carbonate. The research presented in this paper investigated the Soil Water Characteristic Curves (SWCC), permeability coefficient, and microstructure of expansive soil before and after EICP and EICP+ESP modification. A series of laboratory experiments were conducted, including soil water characteristic tests, permeability tests and Scanning Electron Microscopy (SEM). The results proved that the addition of EICP and EICP+ESP into natural expansive soil resulted in a gradual decline in air entry value, residual water content, and permeability coefficient, indicating an increase in water retention capacity and a decrease in permeability. Furthermore, with the intrusion of EICP and EICP+ESP, the contact between particles becomes smoother, and the soil pores become more equally distributed. Ultimately, there was an enhancement in water retention capacity of the natural expansive soil. This study emphasizes the synergistic potential of combining EICP and EICP+ESP as stabilizing additives to enhance the water retention capacity of expansive soil. Moreover, the reuse of ESP provides a sustainable solution for the resource utilization of agricultural waste and the improvement of expansive soil using bio-inspired methods.

Keywords

Enzyme Induced Carbonate Precipitation (EICP) / Eggshell Powder (ESP) / Expansive soil / Soil-water characteristic curves

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Yunlong Liu, Yanyan Xia, Mudassir Mehmood, Lei Wang, Wen Nie, Yingao Zhao, Zhencai Luo. Soil-water retention capacity of expansive soil improved through enzyme induced carbonate precipitation-eggshell powder. Biogeotechnics, 2025, 3(3): 100146 DOI:10.1016/j.bgtech.2024.100146

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CRediT authorship contribution statement

Yunlong Liu: Visualization, Supervision, Resources, Project administration, Funding acquisition, Conceptualization. Mudassir Mehmood: Writing - review & editing, Methodology, Investigation, Data curation, Conceptualization. Yanyan Xia: Writing - original draft, Methodology, Investigation, Formal analysis. Zhencai Luo: Methodology, Formal analysis, Data curation. Yingao Zhao: Validation, Methodology, Investigation, Data curation. Wen Nie: Visualization, Validation, Supervision. Lei Wang: Writing - review & editing, Supervision, Investigation.

Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgments

This work was supported by the Nation Natural Science Foundation of China Youth Project (No. 42107196).

References

[1]

AASHTO T. 27 (2014). Standard Method of Test for Sieve Analysis of Fine and Coarse Aggregates. Washington, DC: American Association of State Highway and Transportation Officials.

[2]

Ahenkorah, I., Rahman, M. M., Karim, M. R., & Beecham, S. (2021). Enzyme induced calcium carbonate precipitation and its engineering application: A systematic review and meta-analysis. Construction and Building Materials, 308, Article 125000. https://doi.org/10.1016/j.conbuildmat.2021.125000

[3]

Al-Taie, A., Disfani, M., Evans, R., Arulrajah, A., & Horpibulsuk, S. (2019). Volumetric behavior and soil water characteristic curve of untreated and lime-stabilized reactive clay. International Journal of Geomechanics, 19(2), Article 04018192. https://doi.org/10.1061/(ASCE)GM.1943-5622.0001336

[4]

ASTM D1883, (2016). Standard Test Method for California Bearing Ratio (CBR) of Laboratory-Compacted Soils, ASTM International, West Conshohocken, Pa.

[5]

ASTM D2166, (2006). Standard Test Method for Unconfined Compressive Strength of Cohesive Soil, ASTM International, West Conshohocken, Pa.

[6]

ASTM D2435, (2003). Standard test methods for one-dimensional swell or settlement potential of cohesive soils ASTM International, West Conshohocken, Pa.

[7]

ASTM D2487, (2017). Standard Practice for Classification of Soils for Engineering Purposes (Unified Soil Classification System), ASTM International, West Conshohocken, Pa.

[8]

ASTM D3080, (2011). Standard test method for direct shear test of soils under consolidated drained conditions, ASTM International, West Conshohocken, Pa.

[9]

ASTM D5084, (2016). Standard test methods for measurement of hydraulic conductivity of saturate porous material using a flexible wall permeability, ASTM International, West Conshohocken, Pa.

[10]

ASTM D5298-10, (2010). Standard Test Method for Measurement of Soil Potential (Suction) Using Filter Paper., ASTM International, West Conshohocken, Pa.

[11]

ASTM D6836-16, (2016). Standard Test Methods for Determination of the Soil Water Characteristic Curve for Desorption Using Hanging Column, Pressure Extractor, Chilled Mirror Hygrometer, or Centrifuge, ASTM International, West Conshohocken, Pa.

[12]

ASTM D698, (2012). Standard Test Methods for Laboratory Compaction Characteristics of Soil Using Standard Effort, ASTM International, West Conshohocken, Pa.

[13]

ASTM D7928, (2010). Standard test method for particle-size distribution (Gradation) of fine-grained soils using the sedimentation (hydrometer) analysis, ASTM international, West Conshohocken, Pa.

[14]

ASTM D854, (2006). Standard test methods for specific gravity of soil solids by water pycnometer, ASTM international, West Conshohocken, Pa.

[15]

ASTM,D4318, (2010). Standard test methods for liquid limit, plastic limit, and plasticity index of soils, ASTM international, West Conshohocken, Pa.

[16]

Boynton, S. S., & Daniel, D. E. (1985). Hydraulic conductivity tests on compacted clay. Journal of Geotechnical Engineering, 111(4), 465-478. https://doi.org/10.1061/(ASCE)0733-9410(1985)111:4(465)

[17]

Chen, L., Chen, X., Yang, X., Bi, P., Ding, X., Huang, X., & Wang, H. (2020). Effect of calcium carbonate on the mechanical properties and microstructure of red clay. Advances in Materials Science and Engineering, 2020, 1-8. https://doi.org/10.1155/2020/5298186

[18]

Chen, Y., Gao, Y., Ng, C. W., & Guo, H. (2021). Bio-improved hydraulic properties of sand treated by soybean urease induced carbonate precipitation and its application Part 1: Water retention ability. Transportation Geotechnics, 27, Article 100489. https://doi.org/10.1016/j.trgeo.2020.100489

[19]

Chen, Y. K., Sun, Y., Wang, K. Q., Kuang, W. Y., Yan, S. R., Wang, Z. H., & Lee, H. S. (2022). Utilization of bio-waste eggshell powder as a potential filler material for cement: Analyses of zeta potential, hydration and sustainability. Construction and Building Materials, 325, Article 126220. https://doi.org/10.1016/j.conbuildmat.2021.126220

[20]

Cui, M. J., Lai, H. J., Hoang, T., & Chu, J. (2021). One-phase-low-pH enzyme induced carbonate precipitation (EICP) method for soil improvement. Acta Geotechnica, 16, 481-489. https://doi.org/10.1007/s11440-020-01043-2

[21]

Elkady, T. Y., Al-Mahbashi, A. M., & Al-Refeai, T. O. (2015). Stress-dependent soil-water characteristic curves of lime-treated expansive clay. Journal of Materials in Civil Engineering, 27(3), Article 04014127. https://doi.org/10.1061/(ASCE)MT.1943-5533.0000995

[22]

Fityus, S., & Buzzi, O. (2009). The place of expansive clays in the framework of unsaturated soil mechanics. Applied Clay Science, 43(2), 150-155. https://doi.org/10.1016/j.clay.2008.08.005

[23]

Gao, Y., He, J., Tang, X., & Chu, J. (2019). Calcium carbonate precipitation catalyzed by soybean urease as an improvement method for fine-grained soil. Soils and Foundations, 59(5), 1631-1637. https://doi.org/10.1016/j.sandf.2019.03.014

[24]

Gourley, C. S., Newill, D., & Schreiner, H. D. (1994). Expansive soils: TRL's research strategy. CRC Press247-260. https://doi.org/10.1201/9781003077787

[25]

Han, Z., Zhang, P., Zou, W., Fan, K., Vanapalli, S. K., & Wan, L. (2024). At-rest lateral earth pressure of compacted expansive soils: Experimental investigations and prediction approach. Journal of Rock Mechanics and Geotechnical Engineering. https://doi.org/10.1016/j.jrmge.2023.10.014

[26]

Hou, T. S., Xu, G. L., Shen, Y. J., Wu, Z. Z., Zhang, N. N., & Wang, R. (2013). Formation mechanism and stability analysis of the Houba expansive soil landslide. Engineering Geology, 161, 34-43. https://doi.org/10.1016/j.enggeo.2013.04.010

[27]

Ikeagwuani, C. C., & Nwonu, D. C. (2019). Emerging trends in expansive soil stabilization: A review. Journal of rock mechanics and geotechnical engineering, 11(2), 423-440. https://doi.org/10.1016/j.jrmge.2018.08.013

[28]

Kanniyappan, S. P., Kumar, R. D., Faizuneesa, A., & Saranya, S. (2019). Experimental investigation on black cotton soil using bio-enzyme as a soil stabilizer in road construction. International Journal of Civil Engineering and Technology (IJCIET).

[29]

Li, J., Bi, W., Yao, Y., & Liu, Z. (2023). State-of-the-art review of utilization of microbial-induced calcite precipitation for improving moisture-dependent properties of unsaturated soils. Applied Sciences, 13(4), 2502. https://doi.org/10.3390/app13042502

[30]

Li, J. H., & Zhang, L. M. (2011). Study of desiccation crack initiation and development at ground surface. Engineering Geology, 123(4), 347-358. https://doi.org/10.1016/j.enggeo.2011.09.015

[31]

Liu, B., Tang, C. S., Pan, X. H., Zhu, C., Cheng, Y. J., Xu, J. J., & Shi, B. (2021). Potential drought mitigation through microbial induced calcite precipitation-MICP. e2020WR029434 Water Resources Research, 57(9), https://doi.org/10.1029/2020WR029434

[32]

Liu, Y., Zhao, Y., Vanapalli, S. K., & Mehmood, M. (2024). Soil-water characteristic curve of expansive soils considering cumulative damage effects of wetting and drying cycles. Engineering Geology, 339, Article 107642. https://doi.org/10.1016/j.enggeo.2024.107642

[33]

Liu, B., Zhu, C., Tang, C. S., Xie, Y. H., Yin, L. Y., Cheng, Q., & Shi, B. (2020). Bio-remediation of desiccation cracking in clayey soils through microbially induced calcite precipitation (MICP). Engineering geology, 264, Article 105389. https://doi.org/10.1016/j.enggeo.2019.105389

[34]

Mehmood, M., Guo, Y., Liu, Y., & Uge, B. U. (2024a). Modification of expansive soil characteristics by employing agro-waste eggshell powder: An experimental study. Iranian Journal of Science and Technology, Transactions of Civil Engineering, 1-16. https://doi.org/10.1007/s40996-023-01284-7

[35]

Mehmood, M., Guo, Y., Liu, Y., Wang, L., Nie, W., Uge, B. U., Ali, S., Chen, X., & Zhao, Y. (2025). Experimental study on the engineering characteristics of expansive soil improved conjointly using enzyme induced carbonate precipitation and eggshell powder. Soils and Foundations, 65(1), 101567. https://doi.org/10.1016/j.sandf.2025.101567

[36]

Mehmood, M., Guo, Y., Wang, L., Liu, Y., Uge, B. U., & Ali, S. (2024b). Influence of enzyme induced carbonate precipitation (EICP) on the engineering characteristics of expansive soil. Arabian Journal for Science and Engineering, 1-16. https://doi.org/10.1007/s13369-024-08896-9

[37]

Meng, H., Gao, Y., He, J., Qi, Y., & Hang, L. (2021). Microbially induced carbonate precipitation for wind erosion control of desert soil: Field-scale tests. Geoderma, 383, Article 114723. https://doi.org/10.1016/j.geoderma.2020.114723

[38]

Miftah, A., Tirkolaei, H. K., & Bilsel, H. (2020). Bio-precipitation of CaCO3 for soil improvement:A Review. In IOP Conference Series: Materials Science and Engineering (Article 012037). IOP Publishing.

[39]

Ng, C. W. W., Guo, H., Ni, J., Zhang, Q., & Chen, Z. (2022). Effects of soil-plant-biochar interactions on water retention and slope stability under various rainfall patterns. Landslides, 19(6), 1379-1390. https://doi.org/10.1007/s10346-022-01874-y

[40]

Ng, C. W. W., Zhang, Q., Zhang, S., Lau, S. Y., Guo, H., & Li, Z. (2024). A new state-dependent constitutive model for cyclic thermo-mechanical behaviour of unsaturated vegetated soil. Canadian Geotechnical Journal. https://doi.org/10.1139/cgj-2023-0268

[41]

Pan, X., Chu, J., & Cheng, L. (2023). Reduction of rainfall infiltration in soil slope using a controllable biocementation method. Biogeotechnics, 1(2), Article 100023. https://doi.org/10.1016/j.bgtech.2023.100023

[42]

Pei, P., Zhao, Y., Ni, P., & Mei, G. (2020). A protective measure for expansive soil slopes based on moisture content control. Engineering Geology, 269, Article 105527. https://doi.org/10.1016/j.enggeo.2020.105527

[43]

Pei, Q. Y., Zou, W. L., Han, Z., Wang, X. Q., & Xia, X. L. (2024). Compression behaviors of a freeze-thaw impacted clay under saturated and unsaturated conditions. Acta Geotechnica, 1-18. https://doi.org/10.1007/s11440-023-02188-6

[44]

Pratama, E. M., Putra, H., & Syarif, F. (2021, October). Application of calcite precipitation method to increase the shear strength of peat soil. In IOP Conference Series: Earth and Environmental Science. IOP Publishing, Article 012058. https://doi.org/10.1088/1755-1315/871/1/012058871.

[45]

Roksana, K., Hewage, S. A., Lomboy, M. M., Tang, C., Xue, W., & Zhu, C. (2023). Desiccation cracking remediation through enzyme induced calcite precipitation in fine-grained soils under wetting drying cycles. Biogeotechnics, 1(4), Article 100049. https://doi.org/10.1016/j.bgtech.2023.100049

[46]

Sahu, G., Singh, A., Kumar, I., & Gupta, D. (2017). Studies on improvement of shear strength of sandy soil using egg shell powder and quarry dust. International Journal of Engineering Research & Technology, 6, 440-443. https://doi.org/10.17577/IJERTV6IS050251

[47]

Salifu, E., MacLachlan, E., Iyer, K. R., Knapp, C. W., & Tarantino, A. (2016). Application of microbially induced calcite precipitation in erosion mitigation and stabilization of sandy soil foreshore slopes: A preliminary investigation. Engineering Geology, 201, 96-105. https://doi.org/10.1016/j.enggeo.2015.12.027

[48]

Sathiparan, N. (2021). Utilization prospects of eggshell powder in sustainable construction material-A review. Construction and Building Materials, 293, Article 123465. https://doi.org/10.1016/j.conbuildmat.2021.123465

[49]

Sillers, W. S., Fredlund, D. G., & Zakerzadeh, N. (2001). Mathematical attributes of some soil—water characteristic curve models. Unsaturated soil concepts and their application in geotechnical practice, 243-283. https://doi.org/10.1007/978-94-015-9775-3_3

[50]

Tay, Y. Y., Stewart, D. I., & Cousens, T. W. (2001). Shrinkage and desiccation cracking in bentonite-sand landfill liners. Engineering Geology, 60(1-4), 263-274. https://doi.org/10.1016/S0013-7952(00)00107-1

[51]

Wang, Y., Konstantinou, C., Tang, S., & Chen, H. (2023). Applications of microbial-induced carbonate precipitation: A state-of-the-art review. Biogeotechnics, Article 100008. https://doi.org/10.1016/j.bgtech.2023.100008

[52]

Wang, X., Xu, C., Wang, S., & Li, X. (2020). Study on overburden pressure effect of expansive soil-water characteristic curve. Chinese Journal of Rock Mechanics and Engineering, 39, 3067-3075. https://doi.org/10.13722/j.cnki.jrme.2019.0895

[53]

Wang, Z., Zhang, N., Cai, G., Jin, Y., Ding, N., & Shen, D. (2017). Review of ground improvement using microbial induced carbonate precipitation (MICP). Marine Georesources Geotechnology, 35(8), 1135-1146. https://doi.org/10.1080/1064119X.2017.1297877

[54]

Xiao, Y., Chen, H., Stuedlein, A. W., Evans, T. M., Chu, J., Cheng, L.,... Aboel-Naga, H. M. (2020). Restraint of particle breakage by biotreatment method. Journal of Geotechnical and Geoenvironmental Engineering, 146(11), Article 04020123. https://doi.org/10.1061/(ASCE)GT.1943-5606.0002384

[55]

Xiao, Y., Zhao, C., Cui, H., Chen, Y., Wu, B., & Liu, H. (2024). Microscale insights into enzyme-induced carbonate precipitation in rock-based microfluidic chips. Géotechnique, 1-12. https://doi.org/10.1680/jgeot.24.01104

[56]

Xu, K., Huang, M., Liu, Z., Cui, M., & Li, S. (2023). Mechanical properties and disintegration behavior of EICP-reinforced sea sand subjected to drying-wetting cycles. Biogeotechnics, Article 100019. https://doi.org/10.1016/j.bgtech.2023.100019

[57]

Yang, H., He, C., Xijao, J., & Zhan, W. (2011). Analysis on improvement effect of expansive soil by soil-water characteristic curve. In Instrumentation, Testing, and Modeling of Soil and Rock Behavior, 272-279. https://doi.org/10.1061/47633(412)36

[58]

Yu, X., Xiao, H., Li, Z., Qian, J., Luo, S., & Su, H. (2021). Experimental study on microstructure of unsaturated expansive soil improved by MICP method. Applied Sciences, 12(1), 342. https://doi.org/10.3390/app12010342

[59]

Zada, U., Jamal, A., Iqbal, M., Eldin, S. M., Almoshaogeh, M., Bekkouche, S. R., & Almuaythir, S. (2023). Recent advances in expansive soil stabilization using admixtures: current challenges and opportunities. Case Studies in Construction Materials, Article e01985. https://doi.org/10.1016/j.cscm.2023.e01985

[60]

Zhang, H., Adoko, A. C., Meng, Z., Wang, H., & Jiao, Y. (2017). Mechanism of the mudstone tunnel failures induced by expansive clay minerals. Geotechnical and Geological Engineering, 35, 263-275. https://doi.org/10.1007/s10706-016-0102-y

[61]

Zhang, J., Wang, X., Shi, L., & Yin, Y. (2022). Enzyme-induced carbonate precipitation (EICP) combined with lignin to solidify silt in the Yellow River flood area. Construction and Building Materials, 339, Article 127792. https://doi.org/10.1016/j.conbuildmat.2022.127792

[62]

Zhen, Y., Kazunori, N., Chikara, T.,Satoru, & K (2024). Feasibility study of enhancing enzyme-induced carbonate precipitation with eggshell waste for sand solidification. Biogeotechnics, Article 100108. https://doi.org/10.1016/j.bgtech.2024.100108

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