Uniformity of microbial injection for reinforcing saturated calcareous sand: A multi-test approach

Xinlei Zhang , Yue Sun , Yumin Chen , Lu Liu , Wenwen Li , Yi Han

Biogeotechnics ›› 2025, Vol. 3 ›› Issue (2) : 100105

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Biogeotechnics ›› 2025, Vol. 3 ›› Issue (2) :100105 DOI: 10.1016/j.bgtech.2024.100105
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Uniformity of microbial injection for reinforcing saturated calcareous sand: A multi-test approach

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Abstract

The mineralization process of microbial-induced calcium carbonate precipitation (MICP) is influenced by many factors, and the uniformity of the calcium carbonate precipitation has become the main focus and challenge for MICP technology. In this study, the uniformity of the saturated calcareous sand treated with MICP was investigated through one-dimensional calcareous sand column tests and model tests. The coefficient of variation was employed in one-dimensional sand column tests to investigate the impact of injection rate, cementation solution concentration, and number of injection cycles on the uniformity of the MICP treatment. Additionally, model tests were conducted to investigate the impact of injection pressure and methods on the treatment range and uniformity under three-dimensional seepage conditions. Test results demonstrate that the reinforcement strength and uniformity are significantly influenced by the injection rate of the cementation solution, with a rate of 3 mL/min, yielding a favorable treatment effect. Excessive concentration of the cementation solution can lead to significant non-uniformity and a reduction in the compressive strength of MICP-treated samples. Conversely, excessively low concentrations may result in decreased bonding efficiency. Among the four considered concentrations, 0.5 mol/L and 1 mol/L exhibit superior reinforcing effects. The morphological development of calcareous sandy foundation reinforcement is associated with the spatial distribution pattern of the bacterial solution, exhibiting a relatively larger reinforcement area in proximity to the lower region of the model and a gradually decreasing range towards the upper part. Under three-dimensional seepage conditions, in addition to the non-uniform radial cementation along the injection pipe, there is also vertical heterogeneity of cementation along the length of the injection pipe due to gravitational effects, resulting in preferential deposition of calcium carbonate at the lower section. The application of injection pressure and a double-pipe circulation injection method can mitigate the accumulation of bacterial solution and cementation solution at the bottom, thereby improving the reinforcement range and uniformity.

Keywords

MICP / Calcareous sand / Reinforcement uniformity / One-dimensional sand column tests / Model tests

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Xinlei Zhang, Yue Sun, Yumin Chen, Lu Liu, Wenwen Li, Yi Han. Uniformity of microbial injection for reinforcing saturated calcareous sand: A multi-test approach. Biogeotechnics, 2025, 3(2): 100105 DOI:10.1016/j.bgtech.2024.100105

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CRediT authorship contribution statement

Xinlei Zhang: Writing - original draft, Conceptualization. Yue Sun: Visualization, Validation. Yumin Chen: Formal analysis, Data curation. Lu Liu: Investigation, Funding acquisition. Wenwen Li: Project administration, Methodology. Yi Han: Software, Resources.

Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgments

The financial support of Natural Science Foundation of China (Grant No. 52108324, No. 52008207, and No. 52108298) for conducting this study.

References

[1]

Al Qabany, A., & Soga, K. (2013). Effect of chemical treatment used in MICP on engineering properties of cemented soils. Ge´otechnique, 63, 331-339. https://doi.org/10.1680/bcmpge.60531.010

[2]

Al-Thawadi, S., & Cord-Ruwisch, R. (2012). Calcium carbonate crystals formation by ureolytic bacteria isolated from Australian soil and sludge. J Adv Sci Eng Res, 2, 12-26.

[3]

Burbank, M., Weaver, T., Lewis, R., Williams, T., Williams, B., & Crawford, R. (2013). Geotechnical tests of sands following bioinduced calcite precipitation catalyzed by indigenous bacteria. J Geotech Geoenvironmental Eng, 139, 928-936. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000781

[4]

Cheng, L., & Shahin, M. A. (2016). Urease active bioslurry: A novel soil improvement approach based on microbially induced carbonate precipitation. Can Geotech J, 53, 1376-1385. https://doi.org/10.1139/cgj-2015-0635

[5]

Cheng, L., Shahin, M. A., & Chu, J. (2019). Soil bio-cementation using a new one-phase low-pH injection method. Acta Geotech, 14, 615-626. https://doi.org/10.1007/s11440-018-0738-2

[6]

Cui, M., Zheng, J., Zhang, R., Miao, C., & Miao, C. (2015). Study of effect of chemical treatment on strength of bio-cemented sand. Rock and Soil Mechanics, 36, 392-396. https://doi.org/10.16285/j.rsm.2015.S1.068

[7]

Cunningham, A. B., Gerlach, R., Spangler, L., Mitchell, A. C., Parks, S., & Phillips, A. (2011). Reducing the risk of well bore leakage of CO2 using engineered biomineralization barriers. Energy Procedia, 4, 5178-5185. https://doi.org/10.1016/j.egypro.2011.02.495

[8]

DeJong, J. T., Martinez, B. C., Ginn, T. R., Hunt, C., Major, D., Tanyu, B., et al. (2014). Development of a Scaled Repeated Five-Spot Treatment Model for Examining Microbial Induced Calcite Precipitation Feasibility in Field Applications. Geotech Test J, 37, 423-435. https://doi.org/10.1520/GTJ20130089

[9]

Fang, X., Yang, Y., Chen, Z., Liu, H., Xiao, Y., & Shen, C. (2020). Influence of Fiber Content and Length on Engineering Properties of MICP-Treated Coral Sand. Geomicrobiol J, 37, 582-594. https://doi.org/10.1080/01490451.2020.1743392

[10]

Fu, T., Saracho, A. C., & Haigh, S. K. (2023). Microbially induced carbonate precipitation (MICP) for soil strengthening: A comprehensive review. Biogeotechnics, 1, Article 100002. https://doi.org/10.1016/j.bgtech.2023.100002

[11]

Hammes, F., & Verstraete, W. (2022). Key roles of pH and calcium metabolism in microbial carbonate precipitation. Rev Environ Sci Biotechnol, 1, 3-7.

[12]

Han, Z., Cheng, X., & Ma, Q. (2016). An experimental study on dynamic response for MICP strengthening liquefiable sands. Earthq Eng Eng Vib, 15, 673-679. https://doi.org/10.1007/s11803-016-0357-6

[13]

Harkes, M. P., van Paassen, L. A., Booster, J. L., Whiffin, V. S., & van Loosdrecht, M. C. M. (2010). Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecol Eng, 36, 112-117. https://doi.org/10.1016/j.ecoleng.2009.01.004

[14]

Hou Shi-Tao, Huang Shi-Ming, Shou-Zhi Li, et al. (1994). Regulations for Pocket Penetrometer Testing (CECS 54:93)[J]. Standardization of engineering construction, (04), 22-31 (in Chinese).

[15]

Lai, Han-Jiang, Cui, Ming-Juan, & Chu, Jian (2023). Effect of pH on soil improvement using One-phase-low-pH MICP or EICP biocementation method[J]. Acta Geotechnica, 18(6), 3259-3272.

[16]

Lai, Han-Jiang, Cui, Ming-Juan, Wu, Shi-Fan, Yang, Yang, & Chu, Jian (2021). Retarding Effect of Concentration of Cementation Solution on Bio-cementation of Soil[J]. Acta Geotechnica, 16(5), 1457-1472.

[17]

Li, Y., Guo, Z., Wang, L., Ye, Z., Shen, C., & Zhou, W. (2021). Interface Shear Behavior between MICP-Treated Calcareous Sand and Steel. J Mater Civ Eng, 33, 1-16. https://doi.org/10.1061/(asce)mt.1943-5533.0003549

[18]

Martinez, B. C., Dejong, J. T., Ginn, T. R., Montoya, B. M., Barkouki, T. H., Hunt, C., et al. (2013). Experimental Optimization of Microbial-Induced Carbonate Precipitation for Soil Improvement. J Geotech Geoenvironmental Eng, 139, 587-598. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000787

[19]

van Paassen, L. A., Ghose, R., & van Loosdrecht, M. C. M. (van der Linden, T. J. M., van der Star, W. R. L., 2010). Quantifying Biomediated Ground Improvement by Ureolysis: Large-Scale Biogrout Experiment. J Geotech Geoenvironmental Eng, 136, 1721-1728. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000382

[20]

Van Paassen, L. A., Harkes, M. P., Van Zwieten, G. A., Van Der Zon, W. H., & Van Loosdrecht, M. C. M. (Van Der Star, W. R. L., 2009). Scale up of BioGrout: A biological ground reinforcement method. Proc 17th Int Conf Soil Mech Geotech Eng Acad Pract Geotech Eng, 3, 2328-2333. https://doi.org/10.3233/978-1-60750-031-5-2328

[21]

Rodriguez-Navarro, C., Rodriguez-Gallego, M., Chekroun K. Ben, & Gonzalez-Muñoz, M. T. (2003). Conservation of ornamental stone by Myxococcus xanthus-induced carbonate biomineralization. Appl Environ Microbiol, 69, 2182-2193. https://doi.org/10.1128/AEM.69.4.2182-2193.2003

[22]

Shao, G., Liu, P., & Hou, M. (2019). Distribution and fixation characteristics of microorganism in MICP treated silt column. Journal of Forestry Engineering, 4, 128-134. https://doi.org/10.13360/j.issn.2096-1359.2019.01.019

[23]

Sharma, M., Satyam, N., & Reddy, K. R. (2021). State of the Art Review of Emerging and Biogeotechnical Methods for Liquefaction Mitigation in Sands. J Hazardous, Toxic, Radioact Waste, 25. https://doi.org/10.1061/(asce)hz.2153-5515.0000557

[24]

Stocks-Fischer, S., Galinat, J. K., & Bang, S. S. (1999). Microbiological precipitation of CaCO3. Soil Biol Biochem, 31, 1563-1571. https://doi.org/10.1016/S0038-0717(99)00082-6

[25]

Sun, X., Miao, L., Chen, R., Wang, H., Wu, L., & Xia, J. (2021). Liquefaction Resistance of Biocemented Loess Soil. J Geotech Geoenvironmental Eng, 147, 1-17. https://doi.org/10.1061/(asce)gt.1943-5606.0002638

[26]

Tian, Z., Tang, X., Li, J., Xiu, Z., & Xue, Z. (2021). Improving settlement and reinforcement uniformity of marine clay in electro-osmotic consolidation using microbially induced carbonate precipitation. Bull Eng Geol Environ, 80, 6457-6471. https://doi.org/10.1007/s10064-021-02305-3

[27]

Tobler, D. J., Cuthbert, M. O., Greswell, R. B., Riley, M. S., Renshaw, J. C., Handley-sidhu, S., et al. (2011). Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite. Geochim Cosmochim Acta, 75, 3290-3301. https://doi.org/10.1016/j.gca.2011.03.023

[28]

Torkzaban, S., Tazehkand, S. S., Walker, S. L., & Bradford, S. A. (2008). Transport and fate of bacteria in porous media: Coupled effects of chemical conditions and pore space geometry. Water Resour Res, 44, 1-12. https://doi.org/10.1029/2007WR006541

[29]

UK, G., CL, K., SS, B., & MR, I. (1995). A new method for controlling leaching through permeable channels. Chemosphere, 30, 695-705. https://doi.org/10.1016/0045-6535(94)00435-W

[30]

Wang, S., Shen, T., Tian, R., & Li, X. (2023b). Uniformity evaluation and improvement technology of sandy clayey purple soil enhanced through microbially-induced calcite precipitation. Biogeotechnics, 1, Article 100048. https://doi.org/10.1016/j.bgtech.2023.100048

[31]

Wang, Y., Konstantinou, C., Tang, S., & Chen, H. (2023a). Applications of microbial- induced carbonate precipitation: A state-of-the-art review. Biogeotechnics, 1, Article 100008. https://doi.org/10.1016/j.bgtech.2023.100008

[32]

Whiffin, V. S., van Paassen, L. A., & Harkes, M. P. (2007). Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol J, 24, 417-423. https://doi.org/10.1080/01490450701436505

[33]

Xiao, P., Liu, H., Stuedlein, A. W., Evans, T. M., & Xiao, Y. (2019). Effect of relative density and biocementation on cyclic response of calcareous sand. Can Geotech J, 1862, 1849-1862.

[34]

Xiao, Y., Wang, Y., Wang, S., Evans, T. M., Stuedlein, A. W., Chu, J.,... Liu, H. (2021). Homogeneity and mechanical behaviors of sands improved by a temperature-controlled one-phase MICP method. Acta Geotechnica, 16, 1417-1427.

[35]

Zhang, Y. S., Liu, Y., Sun, X. D., Zeng, W., Xing, H. P., Lin, J. Z., et al. (2024). Application of microbially induced calcium carbonate precipitation (MICP) technique in concrete crack repair: A review. Constr Build Mater, 411. https://doi.org/10.1016/j.conbuildmat.2023.134313

[36]

Zhong, L., & Islam, M. R. (1995). New microbial plugging process and its impact on fracture remediation. Proc. - SPE Annu. Tech. Conf. Exhib., vol. Delta. USA: Society of Petroleum Engineer,703-715. https://doi.org/10.2523/30519-ms

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