How much biochar should be used in global ecological restoration?

Abstract

Biochar applied in ecological restoration shows a significant dose-dependent effect. Therefore, determining an appropriate rate for specific environments is essential in practical applications. To this end, we integrated soil profiles and biogeochemical and microbiome datasets from six major ecosystems (agricultural, grassland, forest, coastal wetlands, desert, and polar tundra), constructed a global biochar remediation threshold map, and investigated the driving mechanisms of threshold formation. Simultaneously, differentiated application frequency strategies tailored to each ecosystem are proposed. The results showed that the appropriate thresholds for different ecosystems were 5–30 t ha⁻¹ (agricultural), 5–40 t ha⁻¹ (grassland), 5–40 t ha⁻¹ (forest), 10–50 t ha⁻¹ (coastal wetlands), 10–40 t ha⁻¹ (desert), and 20–60 t ha⁻¹ (polar tundra). Within the threshold range, in combination with customized application frequency, biochar enhances ecological functions by increasing soil water-holding capacity (by approximately 10–14.3%), reducing greenhouse gas emissions (by approximately 16.4–31.5%), lowering soil heavy metal content, and increasing soil organic matter. Exceeding the threshold can cause sharp fluctuations in soil pH, increases in bioavailable polycyclic aromatic hydrocarbons, and decreases in microbial diversity, thereby inhibiting remediation. Inadequate application frequency also weakens the ecological restoration efficacy of biochar. The thresholds are environmentally dependent. The threshold window can be expanded or narrowed by the joint regulation of preparation parameters, soil characteristics, climate, and human management. Based on this, we propose a framework of “threshold identification–mechanism analysis–targeted intervention” and customized application paths for ecosystems (e.g., on-farm biochar + fertilizer single/strip application, low-dose staged application in grassland, forest low-dose dispersal single intervention, combination of wetland surface and spot combined with seasonal application, desert inter-root precision single application, and zoned management of medium and low doses in polar regions). The framework provides a quantitative and mechanistic basis for formulating standards for the production and application of biochar and for promoting precise remediation.

Graphical Abstract

Keywords

Biochar / Application threshold / Application frequency / Ecological restoration / Mechanism analysis / Precision regulation

References

[1] Acharya BS, Dodla S, Wang JJ, Pavuluri K, Darapuneni M, Dattamudi S, Kharel G. Biochar impacts on soil water dynamics: knowns, unknowns, and research directions. Biochar, 2024, 6(1): Article ID: 34

[2] Adekiya AO, Ogunbode TO, Esan VI, Adedokun O, Olatubi IV, Ayegboyin MH. Short term effects of biochar on soil chemical properties, growth, yield, quality, and shelf life of tomato. Sci Rep, 2025, 15(1): Article ID: 24965

[3] Alaboudi K (2020) Challenges of biochar usages in arid soils: a case study in the Kingdom of Saudi Arabia. In A. A. Abdelhafez & M. Abbas (Eds.), Applications of biochar for environmental safety. IntechOpen. https://doi.org/10.5772/intechopen.92710

[4] Amirahmadi E, Ghorbani M, Adani F. Biochar contribution in greenhouse gas mitigation and crop yield considering pyrolysis conditions, utilization strategies and plant type - a meta-analysis. Field Crops Res, 2025, 333 Article ID: 110040

[5] Bai X, Du C, Yue C, et al. Weathering geography: a new interdisciplinary subject to interpret biogeochemical cycles in critical regions of the Earth (in Chinese). Chin Sci Bull, 2026.https://doi.org/10.1360/CSB-2025-5745

[6] Bolan N, Hoang SA, Beiyuan J, Gupta S, Hou D, Karakoti A, Van Zwieten L. Multifunctional applications of biochar beyond carbon storage. Int Mater Rev, 2022, 67(2): 150-200

[7] Cai JF, Jiang F, Liu XS, Sun K, Wang W, Zhang MX, Yu FH. Biochar-amended coastal wetland soil enhances growth of Suaeda salsa and alters rhizosphere soil nutrients and microbial communities. Sci Total Environ, 2021, 788(10): Article ID: 147707

[8] Cen R, Feng W, Yang F, Wu W, Liao H, Qu Z. Effect mechanism of biochar application on soil structure and organic matter in semi-arid areas. J Environ Manage, 2021, 286 Article ID: 112198

[9] Chamoli A, Karn SK, Kumari M, Sivaramasamy E. Biochar mediated fixation of nitrogen compounds (ammonia and nitrite) in soil: a review. Biodegradation, 2025, 36(2): Article ID: 22

[10] Chang YB, Liu WG, Mao YQ, Yang T, Chen YG. Biochar addition alters C: N: P stoichiometry in moss crust-soil continuum in Gurbantunggut Desert. Plants, 2022, 11(6): Article ID: 814

[11] Chang L, Ju T, Liang K, Li Y. Biochar-influenced solubilization and mineralization mechanisms of phosphorus in saline-sodic soils. Soil Biol Biochem, 2025, 209 Article ID: 109890

[12] Chávez-García E, González-Méndez B, Molina-Freaner F. Biochar application on mine tailings from arid zones: prospects for mine reclamation. J Arid Environ, 2023, 217(13): Article ID: 105040

[13] Chen GZ, Bai JH, Yu L, Wang W, Wang YQ, Qiu JC, Cui BS. Phragmites australis straw and biochar additives regulate soil organic carbon fractions in a degraded coastal salt marsh. Ecol Eng, 2024, 206(7): Article ID: 107328

[14] Chen X, Liu L, Yang QY, Xu HN, Shen GQ, Chen QC. Optimizing biochar application rates to improve soil properties and crop growth in saline-alkali soil. Sustainability, 2024, 16(6): Article ID: 2523

[15] Cui Q, Xia JB, Peng L, Zhao XM, Qu FZ. Positive effects on alfalfa productivity and soil nutrient status in coastal wetlands driven by biochar and microorganisms mixtures. Frontiers Ecol Evolut, 2022, 9(12): 798520

[16] Curcio R, Bilotti R, Lia C, Compitiello M, Cangemi S, Verrillo M, Mazzei P. Short-term effects of wood biochar on soil fertility, heterotrophic respiration and organic matter composition. Agriculture, 2025

[17] Cybulak M, Sokolowska Z, Boguta P. The influence of biochar on the content of carbon and the chemical transformations of fallow and grassland humic acids. Sci Rep, 2021, 11(1): 14

[18] Dadhich A, Choudhary R, Sharma Y, Dhar I, Jain R. Integrating functional biochar and synthetic microbial consortia for circular bioeconomy and sustainable contaminant remediation. Curr Res Biotechnol, 2025

[19] de la Sota Ricaldi AM, Rengifo Del Águila S, Blas Sevillano R, López-García Á, Corazon-Guivin MA. Beta diversity of arbuscular mycorrhizal communities increases in time after crop establishment of Peruvian Sacha Inchi ( Plukenetia volubilis ). J Fungi, 2023

[20] Diatta AA, Fike JH, Battaglia ML, Galbraith JM, Baig MB. Effects of biochar on soil fertility and crop productivity in arid regions: a review. Arab J Geosci, 2020, 13(14): Article ID: 595

[21] Dong M, Jiang M, He L, Zhang Z, Gustave W, Vithanage M, He F. Challenges in safe environmental applications of biochar: identifying risks and unintended consequence. Biochar, 2025, 7(1): Article ID: 12

[22] Dvořáčková H, Dvořáček J. Manure application followed by biochar application increases plant production regardless of soil dehydrogenase activity. Soil Use Manage, 2023, 39(4): 1557-1569

[23] Fu G, Qiu X, Xu X, Zhang W, Zang F, Zhao C. The role of biochar particle size and application rate in promoting the hydraulic and physical properties of sandy desert soil. CATENA, 2021, 207 Article ID: 105607

[24] Gao S, DeLuca TH. Rangeland application of biochar and rotational grazing interact to influence soil and plant nutrient dynamics. Geoderma, 2022, 408(10): 115572

[25] Gebhardt M, Fehmi JS, Rasmussen C, Gallery RE. Soil amendments alter plant biomass and soil microbial activity in a semi-desert grassland. Plant Soil, 2017, 419(1): 53-70

[26] Glaser B, Lehr V-I. Biochar effects on phosphorus availability in agricultural soils: a meta-analysis. Sci Rep, 2019, 9(1): Article ID: 9338

[27] Gross A, Bromm T, Polifka S, Fischer D, Glaser B. Long-term biochar and soil organic carbon stability—evidence from field experiments in Germany. Sci Total Environ, 2024, 954 Article ID: 176340

[28] Gulfam-E-Jannat S, El Bitar L, Baghirzade BS, Arora U, Sinha N, Dev S, Saleh NB. Unlocking the past: release of hazardous contaminants into water from thawing permafrost. J Hazard Mater, 2025, 495 Article ID: 139068

[29] Han M, Hui X, Yang H, Chen Y. The impact of biochar addition on the carbon footprint of grain crop: a life cycle assessment of biochar management. Eur J Agron, 2025, 170 Article ID: 127776

[30] Hawthorne I, Johnson MS, Jassal RS, Black TA, Grant NJ, Smukler SM. Application of biochar and nitrogen influences fluxes of CO₂, CH₄ and N₂O in a forest soil. J Environ Manage, 2017, 192: 203-214

[31] He Z, Hou RJ, Fu Q, Li TX, Zhang SJ, Su AS. Evolution mechanism of soil hydrothermal parameters under freeze-thaw cycles: Regulatory significance of straw and biochar. J Cleaner Prod, 2023, 385(12): 135787

[32] Hou R, Li T, Fu Q, Liu D, Li M, Zhou Z, Yan J. Effects of biochar and straw on greenhouse gas emission and its response mechanism in seasonally frozen farmland ecosystems. CATENA, 2020, 194 Article ID: 104735

[33] Hou R, Wang L, O'Connor D, Tsang DCW, Rinklebe J, Hou D. Effect of immobilizing reagents on soil Cd and Pb lability under freeze-thaw cycles: implications for sustainable agricultural management in seasonally frozen land. Environ Int, 2020, 144 Article ID: 106040

[34] Hou S, Sun X, Chen G, Siddique KHM, Chen Z, Liu F, Ping S, Lai H, Guo H, An Y, Lin Z, Zhang Z, Sun L, Yang P. Biochar addition mitigates asymmetric competition of water and increases yield advantages of maize–alfalfa strip intercropping systems in a semiarid region on the Loess Plateau. Field Crops Res, 2024, 319 Article ID: 109645

[35] Hu Y, Li Y, Liu K, Shi C, Wang W, Yang Z, Xu K, Li S, Wang Y, Jin L, Wei D, Yan L. Improving the stability of black soil microbial communities through long-term application of biochar to optimize the characteristics of DOM components. Biochar, 2025, 7(1): Article ID: 84

[36] Huang Y, Li L, Yin X, Zhang T. Polycyclic aromatic hydrocarbon (PAH) biodegradation capacity revealed by a genome-function relationship approach. Environ Microbiome, 2023, 18(1): Article ID: 39

[37] Jeffery S, Verheijen FGA, van der Velde M, Bastos AC. A quantitative review of the effects of biochar application to soils on crop productivity using meta-analysis. Agric Ecosyst Environ, 2011, 144(1): 175-187

[38] Jeffery S, van de Voorde TFJ, Harris WE, Mommer L, Van Groenigen JW, De Deyn GB, Ekelund F, Briones MJI, Bezemer TM. Biochar application differentially affects soil micro-, meso-macro-fauna and plant productivity within a nature restoration grassland. Soil Biol Biochem, 2022, 174(11): Article ID: 108789

[39] Joseph S, Cowie AL, Van Zwieten L, Bolan N, Budai A, Buss W, Cayuela ML, Graber ER, Ippolito JA, Kuzyakov Y, Luo Y, Ok YS, Palansooriya KN, Shepherd J, Stephens S, Weng Z (Han), Lehmann J. How biochar works, and when it doesn't: a review of mechanisms controlling soil and plant responses to biochar. GCB Bioenergy, 2021, 13(11): 1731-1764

[40] Khan Z, Zhang K, Khan MN, Bi J, Zhu K, Luo L, Hu L JFIPS (2022) How biochar affects nitrogen assimilation and dynamics by interacting soil and plant enzymatic activities: quantitative assessment of 2 years potted study in a rapeseed-soil system. 13. https://doi.org/10.3389/fpls.2022.853449

[41] Kravchenko E, Dela Cruz TL, Chen XW, Wong MH. Ecological consequences of biochar and hydrochar amendments in soil: assessing environmental impacts and influences. Environ Sci Pollut Res Int, 2024, 31(30): 42614-42639

[42] Kukowska S, Szewczuk-Karpisz K. Management of the soil environment using biochar and zeolite in various combinations: impact on soil condition and economical aspects. J Soils Sediments, 2025, 25(1): 77-102

[43] Latawiec AE, Strassburg BBN, Junqueira AB, Araujo E, de Moraes LFD, Pinto HAN, Castro A, Rangel M, Malaguti GA, Rodrigues AF, Barioni LG, Novotny EH, Cornelissen G, Mendes M, Batista N, Guerra JG, Zonta E, Jakovac C, Hale SE. Biochar amendment improves degraded pasturelands in Brazil: environmental and cost-benefit analysis. Sci Rep, 2019, 9 Article ID: 11993

[44] Latawiec AE, Rodrigues AF, Korys KA, Mendes M, Rangel M, Castro A, Teixeira W, Valentim JF, Araujo E, de Moraes LFD, Pacheco V, Dela Piccolla C, Strassburg B, Kubon M, Drosik A, Gomes FD, Reid B, Krieger JM, Dib V, Zmarzły D, Neto ECdS, Neto ECD. Biochar and forage peanut improve pastures: evidence from a field experiment in Brazil. Agric Ecosyst Environ, 2023, 353(8): Article ID: 108534

[45] Lehmann J, Rillig MC, Thies J, Masiello CA, Hockaday WC, Crowley D. Biochar effects on soil biota – a review. Soil Biol Biochem, 2011, 43(9): 1812-1836

[46] Li J, Shao X, Huang D, Liu K, Shang J, Zhang Q, Zhao T, Yang X. Short-term biochar effect on soil physicochemical and microbiological properties of a degraded alpine grassland. Pedosphere, 2022, 32(3): 426-437

[47] Li X, Wu D, Liu X, Huang Y, Cai A, Xu H, Ran J, Xiao J, Zhang W. A global dataset of biochar application effects on crop yield, soil properties, and greenhouse gas emissions. Sci Data, 2024, 11(1): Article ID: 57

[48] Li C, Lu X, Chen J, et al.. Silicate chemical weathering disrupts the global patterns of phosphorus limitation. Nat Commun, 2025, 16 Article ID: 10742

[49] Li H, Liu H, Qi B, Zhai L, Ding J, Qiu D. Co-application of polyethylene oxide (PEO), biochar, and seaweed fertilizer improves desert soil properties. Sci Rep, 2025, 15(1): Article ID: 1658

[50] Liu X-y, Qu J-J, Li L-Q, Zhang A, Jufeng Z, Zheng J-W, Pan G-X. Can biochar amendment be an ecological engineering technology to depress N ₂ O emission in rice paddies?—A cross site field experiment from South China. Ecol Eng, 2012, 42: 168-173

[51] Long X-X, Yu Z-N, Liu S-w, Gao T, Qiu R-L. A systematic review of biochar aging and the potential eco-environmental risk in heavy metal contaminated soil. J Hazard Mater, 2024, 472 Article ID: 134345

[52] Luo XX, Wang LY, Liu GC, Wang X, Wang ZY, Zheng H. Effects of biochar on carbon mineralization of coastal wetland soils in the Yellow River Delta, China. Ecol Eng, 2016, 94: 329-336

[53] Luo L, Liu Z, Zhu Y-G. Hydrochar: a promising activator for legacy phosphorus in soil. Resour Conserv Recycl, 2023, 197 Article ID: 107113

[54] Majewska M, Hanaka A. Biochar in the bioremediation of metal-contaminated soils. Agronomy, 2025, 15(2): Article ID: 273

[55] McCormack S, Ostle N, Bardgett RD, Hopkins DW, Pereira MG, Vanbergen AJ (2019) Soil biodiversity, carbon cycling and crop plant biomass responses to experimental biochar amendment of agricultural soil (Dundee, UK) NERC Environmental Information Data Centre. https://doi.org/10.5285/130369e1-d9c7-436c-bd0c-1ccde4844576

[56] Minamino Y, Fujitake N, Suzuki T, Yoshitake S, Koizumi H, Tomotsune M. Effect of biochar addition on leaf-litter decomposition at soil surface during three years in a warm-temperate secondary deciduous forest, Japan. Sci Rep, 2019

[57] Nie HD, Qu ZY, Yang W, Wang LP, Zhang RX, Yang YX. Effects of Straw Biochar on Carbon Footprint of Maize Farmland Ecosystem Under Mulched Drip Irrigation in Hetao Irrigation District. Huan Jing Ke Xue, 2023, 44(10): 5832-5841

[58] Odokonyero K, Vernooij B, Albar B, Exposito LO, Alsamdani A, Haider AAG, Musskopf NVH, Kharbatia N, Gallo A, Mishra H. Superhydrophobic sand mulch and date palm biochar boost growth of Moringa oleifera in sandy soils via enhanced irrigation and nutrient use efficiency. Front Plant Sci, 2024, 15 Article ID: 1434462

[59] Pokharel P, Kwak J-H, Ok YS, Chang SX. Pine sawdust biochar reduces GHG emission by decreasing microbial and enzyme activities in forest and grassland soils in a laboratory experiment. Sci Total Environ, 2018, 625: 1247-1256

[60] Qi H, Wang ZH, Lin HX, Song LB, Chen PP, Chen R, Tang YP. Effects of biochar application before and after freeze-thaw on soil hydrothermal and cotton growth under drip irrigation. Water, 2022, 14(23): Article ID: 3818

[61] Roy R, Núñez-Delgado A, Wang JX, Kader MA, Sarker T, Hasan AK, Dindaroglu T. Cattle manure compost and biochar supplementation improve growth of Onobrychis viciifolia in coal-mined spoils under water stress conditions. Environ Res, 2022, 205 Article ID: 112440

[62] Schneckenburger T, Thiele-Bruhn S. Sorption of PAHs and PAH derivatives in peat soil is affected by prehydration status: the role of SOM and sorbate properties. J Soils Sediments, 2020, 20(10): 3644-3655

[63] Shi G, Wu Y, Li T, Fu Q, Wei Y. Mid- and long-term effects of biochar on soil improvement and soil erosion control of sloping farmland in a black soil region, China. J Environ Manage, 2022, 320 Article ID: 115902

[64] Shi J, Pan XY, Zhang WZ, Dong J, Zhao Y, Ran J, Pan X, Zhang W, Zhou D, Li G, Zheng Z. Remediation of coastal wetland soils co-contaminated with microplastics and cadmium using Spartina alterniflora biochar: soil quality, microbial communities, and plant growth responses. Agronomy, 2025, 15(4): Article ID: 877

[65] Shin J, Kim D, Lee Y, Lee S, Wardhani R, Ahn H. Evaluating the odor mitigation effects of biochar-enhanced bedding materials in a simulated bedded pack dairy barn environment: a laboratory-scale study. Appl Sci, 2025

[66] Shukla PR, Skeg J, Buendia EC, Masson-Delmotte V, Pörtner H-O, Roberts D, Van Diemen S (2019) Climate change and land: an IPCC special report on climate change, desertification, land degradation, sustainable land management, food security, and greenhouse gas fluxes in terrestrial ecosystems

[67] Siatecka A, Oleszczuk P. The impact of cyclic freezing and thawing (physical aging) on properties and polycyclic aromatic hydrocarbon content in biochars. Chemosphere, 2023, 331 Article ID: 138760

[68] Sun W, Li Y, Xu Z, Bai Y, Bai SH. Biochar application for enhancing water and nitrogen use efficiency of understory acacia species in a suburban native forest subjected to nitrogen deposition in Southeast Queensland. Plant Soil, 2024, 504(1-2): 607-624

[69] Thomas SC, Gale N. Biochar and forest restoration: a review and meta-analysis of tree growth responses. New for, 2015, 46(5): 931-946

[70] Udomkun P, Rupngam T, Müller J, Masso C, Boonupara T, Kaewlom P. A critical review of regenerative soil management: a novel scoring system for SOC and GHG emissions in Southeast Asia. Soil Use Manage, 2025, 41(3): Article ID: e70118

[71] Walder F, Schmid MW, Riedo J, Valzano-Held AY, Banerjee S, Büchi L, van der Heijden MGA. Soil microbiome signatures are associated with pesticide residues in arable landscapes. Soil Biol Biochem, 2022, 174 Article ID: 108830

[72] Wang K, Wang S, Zhang X, Wang WY, Wang XY, Kong FL, Xi M. The amelioration and improvement effects of modified biochar derived from Spartina alterniflora on coastal wetland soil and Suaeda salsa growth. Environ Res, 2024, 240(12): Article ID: 117426

[73] Wang X, Ding J, Han L, Tan J, Ge X, Nan Q. Biochar addition reduces salinity in salt-affected soils with no impact on soil pH: a meta-analysis. Geoderma, 2024, 443 Article ID: 116845

[74] Wang Z, Yang T, Liu Y, Jiang Q, Shang H, Zheng C. Montmorillonite combined with microbially induced carbonate precipitation for wind erosion control of bare surface soil in arid mining area. Process Saf Environ Prot, 2024, 187: 926-939

[75] Wang ZY, He MX, Lu XQ, Meng ZR, Liu J, Mo XQ. Biochar addition can negatively affect plant community performance when altering soil properties in saline-alkali wetlands. Front Plant Sci, 2024, 15(13): Article ID: 1347658

[76] Wang J, Li X, Li M, Sun H, Hou J, Yang Y, Wu Z. Targeted regulation of biochar-strengthened microorganisms for effective removal of antibiotics from the environment. Clean Technol Environ Policy, 2025

[77] Wei Z, Wang JJ, Meng YL, Li JB, Gaston LA, Fultz LM, DeLaune RD. Potential use of biochar and rhamnolipid biosurfactant for remediation of crude oil-contaminated coastal wetland soil: ecotoxicity assessment. Chemosphere, 2020, 253(9): Article ID: 126617

[78] Wengang L, Fang C, Rong Z, Cuihong C. Biochar-mediated degradation of Roxarsone by Shewanella oneidensis MR-1. Front Microbiol, 2022, 13 Article ID: 846228

[79] Wu J, Deng L, Kuzyakov Y, Huang Y, Song X, Nie M, Shangguan Z. Soil organic and inorganic carbon pools in the Loess Plateau: new estimation, change and trade-offs. Glob Planet Change, 2025, 253 Article ID: 104951

[80] Xiaoli Z, Fanlei K, Xiaolin L, Lifeng H, Yuyi L. Effects of different biomass amendments on soil organic carbon characteristics in alpine desertification grassland of Northwest Sichuan. Chin J Eco-Agric, 2019, 27(11): 1732-1743

[81] Xue P, Fu Q, Li TX, Liu D, Hou RJ, Li QL, Li T, Hou R, Li Q, Li M, Meng F, Meng FX. Effects of biochar and straw application on the soil structure and water-holding and gas transport capacities in seasonally frozen soil areas. J Environ Manage, 2022, 301(12): Article ID: 113943

[82] Xue P, Hou R, Fu Q, Li T, Li M, Liu D, Li Q. Mechanisms of the effects of the biochar application rate and time on farmland water, heat and gas environments during soil thawing in seasonally frozen soil areas: a two-year field trial. J Environ Chem Eng, 2023, 11(5): Article ID: 110928

[83] Yoshitake S, Enichi K, Tsukimori Y, Ohtsuka T, Koizumi H, Tomotsune M. Long-term effects of biochar application on soil heterotrophic respiration in a warm–temperate oak forest. Forests, 2025

[84] Zhang Y, Hou R, Fu Q, Li T, Li M, Dong S, Shi G. Soil environment, carbon and nitrogen cycle functional genes in response to freeze-thaw cycles and biochar. J Clean Prod, 2024, 444 Article ID: 141345

[85] Zhang J, Ge L-a, Yang Y, Zhang X, Wang C, Sun H, Chen H, Huang J, Zhou S. Production and subsequent application of different biochar-based organic fertilizers to enhance vegetable quality and soil carbon stability. J Soil Sci Plant Nutr, 2025, 25(1): 147-159

[86] Zhang Y, Yan C, Wang T, Zhang G, Bahn M, Mo F, Han J. Biochar strategy for long-term N₂O emission reduction: insights into soil physical structure and microbial interaction. Soil Biol Biochem, 2025, 202 Article ID: 109685

[87] Zuo Y, Meng F, Li T, Fu Q, Liu D, Hou R, Li Q, Li M. Effect of biochar application on freezing-thawing deformation of farmland soil during freeze–thaw cycling. Geoderma, 2022, 405 Article ID: 115510

Funding

National Natural Science Foundation(No. U24A20579)

Guizhou Provincial Science and Technology Projects(No. Qiankehe Support [2024] Key 014)

High−level Innovative Talents in Guizhou Province(No. GCC [2022]015−2)

Opening Fund of the State Key Laboratory of Environmental Geochemistry(SKLEG2024202)

Guizhou Province Science and Technology Innovation Talent Team Construction Project(No. Qiankehe Talent CXTD (2025) 057)

Guizhou University Leading Talents Project(No.Guidalingjunhezi (2025) 03)

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The Author(s)