Effect of MnO₂-biochar composites on promoting humification during chicken manure composting

Abstract

The present study aimed to accelerate the humification and to investigate how MnO₂ modification of biochar (MBC) drives the humus formation during composting with chicken manure. In this study, compared with the control group (CK), the addition of MBC caused an increase in the concentration of both humus and humic acid (HA), with a respective enhancement of 29.1% and 37.2%. In addition, MBC also improved the stability of compost products. Hetero two-dimensional correlation spectra further exhibited that the MBC could alter the formation mechanism of humus fractions during composting. Random forest analysis showed that Microbacterium, Bacteroides, Kroppenstedtia, Gracilibacillus, and Lentibacillus were significantly related to humus formation (P < 0.05). MBC enhanced the absolute abundance of these five genera during composting. The structural equation model further confirmed that these five genera could be indirectly involved in humus formation, through the production of aromatic compounds via secondary metabolism. Additionally, these five genera could directly transform organic components into macromolecular humus structures. Therefore, the increase in these five genera might be a direct response to the acceleration of the humification during MBC composting. These findings demonstrate the potential value of MBC in harmless disposal of hazardous biowastes through composting.

Highlights

•	MnO2 modification of biochar changed the formation mechanism of humus fractions.
•	Key genera involved in humus formation were identified.
•	Among of MnO2 modification of biochar, key genera and humus formation were revealed.

Keywords

Composting / MnO₂-biochar / Humus / Key microbes

References

[1] Awasthi MK, Wang Q, Chen HY, Wang MJ, Ren XN, Zhao JC, Li J, Guo D, Li DS, Awasthi SK, Sun XN, Zhang ZQ. Evaluation of biochar amended biosolids co-composting to improve the nutrient transformation and its correlation as a function for the production of nutrient-rich compost. Bioresour Technol, 2017, 237: 156-166,

[2] Chen H, Awasthi SK, Liu T, Duan Y, Ren X, Zhang Z, Pandey A, Awasthi MK. Effects of microbial culture and chicken manure biochar on compost maturity and greenhouse gas emissions during chicken manure composting. J Hazard Mater, 2020, 389,

[3] Chen XM, Du Z, Liu D, Wang LQ, Pan CN, Wei ZM, Jia LM, Zhao R. Biochar mitigates the biotoxicity of heavy metals in livestock manure during composting. Biochar, 2022, 4(1): 48,

[4] El-Naggar AL, Mosa A, Ahmed N, Niazi NK, Yousaf B, Sarkar B, Rinklebe J, Cai YJ, Chang SX. Modified and pristine biochar for remediation of chromium contamination in soil and aquatic systems. Chemosphere, 2022, 303,

[5] Gao XT, Tan WB, Zhao Y, Wu JQ, Sun QH, Qi HS, Xie X, Wei ZM. Diversity in the mechanisms of humin formation during composting with different materials. Environ Sci & Technol, 2019, 53: 3653-3662,

[6] Hardie AG, Dynes JJ, Kozak LM, Huang PM. The role of glucose in abiotic humification pathways as catalyzed by birnessite. J Mol Catal A-Chem, 2009, 308: 114-126,

[7] He XS, Xi BD, Li X, Pan HW, An D, Bai SG, Li D, Cui DY. Fluorescence excitation-emission matrix spectra coupled with parallel factor and regional integration analysis to characterize organic matter humification. Chemosphere, 2013, 93: 2208-2215,

[8] He X, Hu Q, Chen JL, Leong WQ, Dai YJ, Wang CH. Energy and environmental risk assessments of poultry manure sustainable solution: an industrial case study in Singapore. J Clean Prod, 2022, 339,

[9] Ji ZY, Zhang LY, Liu YW, Li XQ, Li ZJ. Evaluation of composting parameters, technologies and maturity indexes for aerobic manure composting: a meta-analysis. Sci Total Environ, 2023, 886,

[10] Kasera N, Hall S, Kolar P. Effect of surface modification by nitrogen-containing chemicals on morphology and surface characteristics of N-doped pine bark biochars. J Environ Chem Eng, 2021, 9,

[11] Liang J, Liu J, Yuan X, Dong H, Zeng G, Wu H, Wang H, Liu J, Hu S, Zhang S, Yu Z, He X, He Y. Facile synthesis of alumina-decorated multi-walled carbon nanotubes for simultaneous adsorption of cadmium ion and trichloroethylene. Chem Eng J, 2015, 273: 101-110,

[12] Liang C, Schimel JP, Jastrow JD. The importance of anabolism in microbial control over soil carbon storage. Nat Microbiol, 2017, 2: 17105,

[13] Liang J, Li X, Yu Z, Zeng G, Luo Y, Jiang L, Yang Z, Qian Y, Wu H. Amorphous MnO₂ modified biochar derived from aerobically composted swine manure for adsorption of Pb(II) and Cd(II). ACS Sustain Chem Eng, 2017, 5: 5049-5058,

[14] Liu J, Yang X, Liu H, Cheng W, Bao Y. Modification of calcium-rich biochar by loading Si/Mn binary oxide after NaOH activation and its adsorption mechanisms for removal of Cu(II) from aqueous solution. Colloid Surfaces A, 2020, 601,

[15] Liu YX, Gao CX, Wang YY, He LL, Lu HH, Yang SM. Vermiculite modification increases carbon retention and stability of rice straw biochar at different carbonization temperatures. J Clean Prod, 2020, 254,

[16] Lodygin ED, Beznosikov VA, Vasilevich RS. Molecular composition of humic substances in tundra soils (C-13-NMR spectroscopic sudy). Eurasian Soil Sci, 2014, 47: 400-406,

[17] Lyu HH, Zhang QR, Shen BX. Application of biochar and its composites in catalysis. Chemosphere, 2020, 240: 124842,

[18] Oldfield TL, Sikirica N, Mondini C, Lopez G, Kuikman PJ, Holden NM. Biochar, compost and biochar-compost blend as options to recover nutrients and sequester carbon. J Environ Manage, 2018, 218: 465-476,

[19] Pan CN, Yang HY, Gao WF, Wei ZM, Song CH, Mi JY. Optimization of organic solid waste composting process through iron-related additives: A systematic review. J Environ Manage, 2024, 351: 119952,

[20] Qi H, Zhang A, Du Z, Wu J, Chen X, Zhang X, Zhao Y, Wei Z, Xie X, Li Y, Ye M. delta-MnO₂ changed the structure of humic-like acid during co-composting of chicken manure and rice straw. Waste Manage, 2021, 128: 16-24,

[21] Rajapaksha AU, Chen SS, Tsang DCW, Zhang M, Vithanage M, Mandal S, Gao B, Bolan NS, Ok YS. Engineered/designer biochar for contaminant removal/immobilization from soil and water: potential and implication of biochar modification. Chemosphere, 2016, 148: 276-291,

[22] Shaheen SM, Natasha, Mosa A, El-Naggar A, Hossain MF, Abdelrahman H, Niazi NK, Shahid M, Zhang T, Tsang YF, Trakal L, Wang S, Rinklebe J. Manganese oxide-modified biochar: production, characterization and applications for the removal of pollutants from aqueous environments a review. Bioresour Technol, 2022, 346: 126581,

[23] Song BA, Yin Z, Almatrafi E, Sang F, Shen MC, Xiong WP, Zhou CY, Liu Y, Zeng GM, Gong JL. When chicken manure compost meets iron nanoparticles: an implication for the remediation of chlorophenothane-polluted riverine sediment. Environ Sci-Nano, 2022, 9: 1519-1529,

[24] Sun R, Zhu XD, Wang C, Yue JY, Pan LN, Song CH, Zhao Y. Effect of NH4+ and NO3− cooperatively regulated carbon to nitrogen ratio on organic nitrogen fractions during rice straw composting. Bioresour Technol, 2024, 395: 13031,

[25] Wang YD, Hu N, Ge TD, Kuzyakov Y, Wang ZL, Li ZF, Tang Z, Chen Y, Wu CY, Lou YL. Soil aggregation regulates distributions of carbon, microbial community and enzyme activities after 23-year manure amendment. Appl Soil Ecol, 2017, 111: 65-72,

[26] Wu D, Yue JY, Gao WF, Wang F, Qu FT, Song CH, Wei ZM. Functional genes are the key factors driving the Fenton-like reactions to promote the hydrolysis of lignocellulosic biomass during composting. Ind Crop Prod, 2024, 210: 118131,

[27] Xiong SG, Liu YD, Zhang H, Xu SQ, Li SR, Fan XQ, Chen R, Ding GC, Li J, Wei YQ. Effects of chemical additives and mature compost on reducing nitrogen loss during food waste composting. Environ Sci Pollut R, 2023, 30: 39000-39011,

[28] Yang WQ, Zhuo Q, Chen QH, Chen ZL. Effect of iron nanoparticles on passivation of cadmium in the pig manure aerobic composting process. Sci Total Environ, 2019, 690: 900-910,

[29] Yuan Y, He XS, Xi BD, Li D, Gao RT, Tan WB, Zhang H, Yang C, Zhao XY. Polarity and molecular weight of compost-derived humic acid affect Fe(III) oxides reduction. Chemosphere, 2018, 208: 77-83,

[30] Zhang M, Gao B, Yao Y, Xue Y, Inyang M. Synthesis of porous MgO-biochar nanocomposites for removal of phosphate and nitrate from aqueous solutions. Chem Eng J, 2012, 210: 26-32,

[31] Zhang CY, Li X, Zhao XY, Yang JJ, Li SK, Dang QL, Tan XK. Recognize and assessment of key host humic-reducing microorganisms of antibiotic resistance genes in different biowastes composts. Sci Total Environ, 2022, 806,

[32] Zhang YY, He MJ, Wang L, Yan JH, Ma B, Zhu XH, Ok YS, Mechtcherine V, Tsang DCW. Biochar as construction materials for achieving carbon neutrality. Biochar, 2022, 4: 59,

[33] Zhang Y, Xu Y, Li J, Chen G, Wang S, Xu Y, Xu R, Zhang B, Zhang H. Microbial metabolism and humic acid formation in response to enhanced copper and zinc passivation during composting of wine grape pomace and pig manure. Bioresour Technol, 2023, 384,

[34] Zhao M, Cai C, Yu Z, Rong H, Zhang C, Zhou S. Effect of biochar on transformation of dissolved organic matter and DTPA-extractable Cu and Cd during sediment composting. Environ Sci Pollut R, 2022, 29: 27977-27987,

[35] Zhao XY, Xu KL, Wang JW, Wang ZQ, Pan RK, Wang Q, Li SL, Kumar S, Zhang ZQ, Li RH. Potential of biochar integrated manganese sulfate for promoting pig manure compost humification and its biological mechanism. Bioresour Technol, 2022, 357,

Funding

National Natural Science Foundation of China(52370148); Natural Science Foundation of Guangxi Province(AD23026037)