Biochar-mediated Cd accumulation in rice grains through altering chemical forms, subcellular distribution, and physiological characteristics

Highlights

•	Biochar decreased alkaline soil available Cd but increased rice Cd accumulation due to increased functional groups in roots
•	Biochar increased soluble fractions and active Cd in rice roots and leaves
•	Hormones, subcellular distribution and chemical forms act together on rice grain Cd accumulation

Abstract

Biochar can change the availability and morphology of soil Cd. However, the influence of biochar on Cd chemical form and subcellular fraction in rice is poorly understood, particularly under different irrigation methods. A pot experiment of biochar application combined with two irrigation methods (continuous flooding and intermittent irrigation, CF and II) was conducted. The Cd accumulation, chemical form and subcellular fraction in rice organs and the associated physiological responses were examined. Biochar significantly reduced soil available Cd (30.85–47.26% and 32.35–52.35%) under CF and II but increased the Cd content (30.4–63.88% and 13.03–18.59%) in brown rice. Additionally, the Cd content in shoots/grains under II was higher than that under CF. Biochar elevated the Cd soluble fraction in roots while lowered the cell wall fraction under both irrigation methods, whereas the opposite result was observed in leaves. Biochar increased water-, ethanol-, and NaCl-extractable Cd in roots meanwhile increased ethanol-extractable Cd in leaves under both irrigation methods. Moreover, the total amount of water-, ethanol-, and NaCl-extractable Cd in rice roots was higher under II than under CF. Related hormones and antioxidant enzymes may also be involved in biochar-mediated Cd accumulation in rice grains. Thus, changes in Cd chemical form and subcellular fraction in the root and leaf are the main mechanisms of biochar-induced rice grains Cd accumulation.

Funding

National Natural Science Foundation of China(32201889); Jiangsu Funding Program for Excellent Postdoctoral Talent(2022ZB627); Jiangsu Technical System of Rice Industry(JATS[2021]485); Special Funds of the Rice Industry System of Jiangxi Province(JXARS-02-03)