Valorization of ligustrum species: biosynthesis, metabolic engineering, and pharmacology of bioactive compounds

Abstract

The genus Ligustrum (Oleaceae) encompasses woody plants with both medicinal and edible uses, distinguished by a wide range of bioactive compounds, including triterpenoids, phenylethanoid glycosides, flavonoids, and other active constituents. These metabolites demonstrate multi-target pharmacological effects, such as anti-inflammatory, antioxidant, antitumor, and anti-osteoporotic activities. In traditional medicine, species like L. lucidum and L. robustum are well-documented for their therapeutic roles in nourishing the liver and kidneys, enhancing vision, darkening hair, and serving as functional tea ingredients. Beyond their medicinal and health-promoting properties, Ligustrum species are also employed as ornamental plants, bioindicators of atmospheric pollution, algicidal agents, and feed additives. Given the increasing global demand and underutilization of Ligustrum resources, there is an urgent need to establish a sustainable supply framework focused on alternative strategies such as metabolic engineering, synthetic biology, and environmentally friendly manufacturing. This review encapsulates recent progress in the exploration of chemical diversity, pharmacological characteristics, omics-based analyses, and biosynthetic research pertaining to Ligustrum. It proposes an integrated methodology that amalgamates multi-omics approaches, synthetic biology, and environmentally sustainable manufacturing processes to advance strategies for whole-plant valorization and germplasm conservation. The objective is to establish a theoretical framework and technical paradigm to facilitate the comprehensive exploitation and sustainable utilization of Ligustrum as a medicinal resource.

Keywords

Ligustrum / Bioactive components / Biosynthesis / Regulation / Metabolic engineering / Pharmacological activity

References

[1] Agar OT, Cankaya IIT (2020) Chapter 5 - analysis of phenylethanoids and their glycosidic derivatives. In: Sanches Silva A, Nabavi SF, Saeedi M, Nabavi SM (eds) Recent Advances in Natural Products Analysis. Elsevier, Netherlands, pp 221–254

[2] Bai Y, Bi H, Zhuang Y, Liu C, Cai T, Liu X, Zhang X, Liu T, Ma Y. Production of salidroside in metabolically engineered Escherichia coli. Sci Rep. 2014, 4: 6640.

[3] Bi F, Bai Y, Zhang Y, Liu W. Ligustroflavone exerts neuroprotective activity through suppression of nlrp1 inflammasome in ischaemic stroke mice. Exp Ther Med. 2023, 25(1): 8.

[4] Bureau J, Oliva ME, Dong Y, Ignea C. Engineering yeast for the production of plant terpenoids using synthetic biology approaches. Nat Prod Rep. 2023, 40121822-1848.

[5] Cao F, Chen M, Feng WJ, Deng XK, Chen JX, Tan H. Ligustrum robustum extract on hypoglycemic effect of mice with type 2 diabetes mellitus. Chin Arch Tradit Chin Med. 2016, 34124

[6] Cao S, Wastney ME, Lachcik PJ, Xiao H, Weaver CM, Wong M. Both oleanolic acid and a mixture of oleanolic and ursolic acids mimic the effects of fructus ligustri lucidi on bone properties and circulating 1,25-dihydroxycholecalciferol in ovariectomized rats. J Nutr. 2018, 148121895-1902.

[7] Cao M, Wu J, Peng Y, Dong B, Jiang Y, Hu C, Yu L, Chen Z. Ligustri lucidi fructus, a traditional chinese medicine: comprehensive review of botany, traditional uses, chemical composition, pharmacology, and toxicity. J Ethnopharmacol. 2023, 301: 115789.

[8] Chen JF, Weng YC. Effects of three processing methods on changes of seven chemical constituents in ligustri lucidi fructus. Mod Chin Med. 2021, 23(7): 1260-1265

[9] Chen B, Wang L, Li L, Zhu R, Liu H, Liu C, Ma R, Jia Q, Zhao D, Niu J, Fu M, Gao S, Zhang D. Fructus ligustri lucidi in osteoporosis: a review of its pharmacology, phytochemistry, pharmacokinetics and safety. Molecules. 2017, 2291469.

[10] Chen N, Li XL, Zhang Y. Research progress on osteoprotective effects of fructus ligustri lucidi and its active components and action pathways involved. Chin Pharmacol Bull. 2018, 34081057-1060

[11] Chen X, Kou Y, Lu Y, Pu Y. Salidroside ameliorated hypoxia-induced tumorigenesis of bxpc-3 cells via downregulating hypoxia-inducible factor (hif)-1alpha and loxl2. J Cell Biochem. 2020, 121(1): 165-173.

[12] Chen L, Huang D, Jiang L, Yang J, Shi X, Wang R, Li W. A review of botany, phytochemistry, pharmacology, and applications of the herb with the homology of medicine and food: ligustrum lucidum w.t. Aiton Front Pharmacol. 2024, 15: 1330732.

[13] Chen X, Li W, Lu X, Tran LP, Alolga RN, Yin X (2024b) Research progress on the biosynthesis, metabolic engineering, and pharmacology of bioactive compounds from the Lonicera genus. Med Plant Biol 3(1):e8

[14] Chen X, Qian T, Wei W, Zhu Y, Cai G, Li M, Chu X, Ye B. De novo synthesis of tyrosol and hydroxytyrosol through temperature-inducible systems and metabolic engineering. ACS Synth Biol. 2025, 14(6): 2294-2304.

[15] Cheng X, Pang Y, Ban Y, Cui S, Shu T, Lv B, Li C. Application of multiple strategies to enhance oleanolic acid biosynthesis by engineered saccharomyces cerevisiae. Bioresour Technol. 2024, 401: 130716.

[16] Chinese PharmacopoeiaCommission. Pharmacopoeia of people’s republic of china: part 1, Part 1 ed. 2025, Beijing, China Medical Science

[17] Dong X, Zhao Q, Wang X (2022) Preliminary results on the separation of the different parts of ligustrum lucidum ait fruit and the main bioactive compounds analysis. Heliyon 8 (12):e12139

[18] Du PF, Sheng YZ, Wang JT, Gan LM, Feng ZL, Chen W. Research progress on metabolic characteristics and pharmacological effects of active ingredients in ligustri lucidi. Chem Reagents. 2024, 4609117-125

[19] Duan LN, Ceng WZ, Sun Y, Zhou JW. Metabolic engineering modification of saccharomyces cerevisiae for efficient synthesis of oleanolic acid. Food Ferment Ind. 2024, 501867-74

[20] Feng R, Ding F, Mi X, Liu S, Jiang A, Liu B, Lian Y, Shi Q, Wang Y, Zhang Y. Protective effects of ligustroflavone, an active compound from ligustrum lucidum, on diabetes-induced osteoporosis in mice: a potential candidate as calcium-sensing receptor antagonist. Am J Chin Med. 2019, 47(2): 457-476.

[21] Feng R, He M, Li Q, Liang X, Tang D, Zhang J, Liu S, Lin F, Zhang Y. Phenol glycosides extract of fructus ligustri lucidi attenuated depressive-like behaviors by suppressing neuroinflammation in hypothalamus of mice. Phytother Res. 2020, 34(12): 3273-3286.

[22] Fu G, Pang H, Wong YH. Naturally occurring phenylethanoid glycosides: potential leads for new therapeutics. Curr Med Chem. 2008, 15(25): 2592-2613.

[23] Gao B, She G, She D. Chemical constituents and biological activities of plants from the genus ligustrum. Chem Biodivers. 2013, 10(1): 96-128.

[24] Gao L, Li C, Wang Z, Liu X, You Y, Wei H, Guo T. Ligustri lucidi fructus as a traditional chinese medicine: a review of its phytochemistry and pharmacology. Nat Prod Res. 2015, 29(6): 493-510.

[25] He Z, Peng Y, Zhang J, Qiu S, Xiao P, Luo J, Li W, Wu X, Li C, Liu Z, Wu H, Hu X, Hao Y, Xu C, Li L. Development and application of Ligustrum (Oleaceae) Kudingcha resources. 2017, Guangdong, Shenzhen University

[26] Hu B, Du Q, Deng S, An H, Pan C, Shen K, Xu L, Wei M, Wang S. Ligustrum lucidum ait. Fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21. Oncol Rep. 2014, 32(3): 1037-1042.

[27] Hu B, Zhao X, Wang E, Zhou J, Li J, Chen J, Du G. Efficient heterologous expression of cytochrome p450 enzymes in microorganisms for the biosynthesis of natural products. Crit Rev Biotechnol. 2023, 43(2): 227-241.

[28] Ji X, Liu X, Gao L, Xiao S, Liang Y, Li C, Wang Z. Qualitative and quantitative analysis on triterpenoids in ligustri lucidi fructus. Zhongguo Zhong Yao Za Zhi. 2021, 46(5): 1168-1178

[29] Jiang J, Zhao B, Ma Y, Zeng Y, Zeng Y, Fang J, Kang W, Chen T. Capture the superior anti-osteoporotic innovative multi-component complex from ligustri lucidi fructus based on component recombination strategy. Phytomedicine. 2025, 146: 157116.

[30] Jin Y, Wang Y, Gao Y, Zhou L, Wang Y, Yuan Q, Dong W. Complete chloroplast genome of ligustrum lucidum and highly variable marker identification for ligustrum. Zhongguo Zhong Yao Za Zhi. 2022, 47(7): 1847-1856

[31] Jin K, Shi X, Liu J, Yu W, Liu Y, Li J, Du G, Lv X, Liu L. Combinatorial metabolic engineering enables the efficient production of ursolic acid and oleanolic acid in saccharomyces cerevisiae. Bioresour Technol. 2023, 374: 128819.

[32] Kang R, Tian W, Cao W, Sun Y, Zhang H, Feng Y, Li C, Li Z, Li X. Ligustroflavone ameliorates ccl(4)-induced liver fibrosis through down-regulating the tgf-beta/smad signaling pathway. Chin J Nat Med. 2021, 193170-180

[33] Kim H, Kong C, Seo Y (2022) Salidroside, 8(e)-nuezhenide, and ligustroside from ligustrum japonicum fructus inhibit expressions of mmp-2 and – 9 in ht 1080 fibrosarcoma. Int.J Mol Sci 23(5):2660

[34] Lai W, Luo R, Tang Y, Yu Z, Zhou B, Yang Z, Brown J, Hong G. Salidroside directly activates hsc70, revealing a new role for hsc70 in bdnf signalling and neurogenesis after cerebral ischemia. Phytother Res. 2024, 3862619-2640.

[35] Li L, Xu L, Peng Y, He Z, Shi R, Xiao P. Simultaneous determination of five phenylethanoid glycosides in small-leaved kudingcha from the ligustrum genus by uplc/pda. Food Chem. 2012, 131(4): 1583-1588.

[36] Li D, Wu Y, Wei P, Gao X, Li M, Zhang C, Zhou Z, Lu W. Metabolic engineering of yarrowia lipolytica for heterologous oleanolic acid production. Chem Eng Sci. 2020, 218: 115529.

[37] Li W, Mai J, Lin L, Zhang Z, Ledesma-Amaro R, Dong W, Ji X. Combination of microbial and chemical synthesis for the sustainable production of β-elemene, a promising plant-extracted anticancer compound. Biotechnol Bioeng. 2023, 120(12): 3612-3621.

[38] Li T, Liu X, Xiang H, Zhu H, Lu X, Feng B. Two-phase fermentation systems for microbial production of plant-derived terpenes. Molecules. 2024, 2951127.

[39] Li Y, Yuan M, Zhang Z, Pei N (2025) The gap-free genome assembly and multi-omics analyses illustrate the evolutionary history and the synthesis of medicinal components of ligustrum lucidum. Plant J 121 (4):e70029

[40] Ling Z, Zeng R, Zhou X, Chen F, Fan Q, Sun D, Chen X, Wei M, Wu R, Luo W (2022) Component analysis using uplc-q-exactive orbitrap-hrms and quality control of kudingcha (ligustrum robustum (roxb.) Blume). Food Res Int 162:111937

[41] Ling ZZ, Ceng R, Fan Q, Wu RS, Li ZY, Chen XD, Sun DM, Luo WH. Kudingcha (ligustrum robustum (roxb.) Blume): structural characteristics of constituents, bioactivities and action mechanisms, and quality control. Food Sci. 2023, 4407394-403

[42] Liu M, Zou Z. Research progress on chemical constituents, pharmacological effects and pharmacokinetics of ligustri lucidi fructus. J Trop Subtropical Bot. 2022, 30(3): 446-460

[43] Liu X, Li X, Jiang J, Liu Z, Qiao B, Li F, Cheng J, Sun X, Yuan Y, Qiao J, Zhao G. Convergent engineering of syntrophic Escherichia coli coculture for efficient production of glycosides. Metab Eng. 2018, 47: 243-253.

[44] Liu MH, Li SL, Zhang L, Wu JH, Zou ZR. Review on research progress of chemical constituents and pharmacological activities of ligustrum. Chin Tradit Herb Drugs. 2020, 51(12): 3337-3348

[45] Liu H, Tian Y, Zhou Y, Kan Y, Wu T, Xiao W, Luo Y. Multi-modular engineering of saccharomyces cerevisiae for high-titre production of tyrosol and salidroside. Microb Biotechnol. 2021, 14(6): 2605-2616.

[46] Liu S, Xia Y, Yang H, Shen W, Chen X. Rational chromosome engineering of Escherichia coli for overproduction of salidroside. Biochem Eng J. 2022, 184: 108474.

[47] Liu Q, Chen J, Zeng A, Song L. Pharmacological functions of salidroside in renal diseases: facts and perspectives. Front Pharmacol. 2023, 141309598.

[48] Lu S, Huang J, Zuo H, Zhou Z, Yang C, Huang Z. Monoterpenoid glycosides from the leaves of ligustrum robustum and their bioactivities. Molecules. 2022, 27(12): 3709.

[49] Lu S, Zuo H, Huang J, Li W, Huang J, Li X. Chemical constituents from the leaves of ligustrum robustum and their bioactivities. Molecules. 2023, 281362.

[50] Lv J, Cao L, Zhang R, Wei P. Anti-diabetic activity of aqueous extract of fructus ligustri lucidi in a rat model of type 2 diabetes. Trop J Pharm Res. 2018, 1771373-1377.

[51] Ma C, Zhou X, Xu K, Wang L, Yang Y, Wang W, Liu A, Ran J, Yan S, Wu H, Wu L. Specnuezhenide decreases interleukin-1beta-induced inflammation in rat chondrocytes and reduces joint destruction in osteoarthritic rats. Front Pharmacol. 2018, 9: 700.

[52] Mai J, Liu A, Li W, Lin L, Sun M, Wang K, Ji X. Biotechnological production of carotenoids using yarrowia lipolytica. J Agric Food Chem. 2025, 73127034-7045

[53] Marti M, Diretto G, Aragones V, Frusciante S, Ahrazem O, Gomez-Gomez L, Daros J. Efficient production of saffron crocins and picrocrocin in nicotiana benthamiana using a virus-driven system. Metab Eng. 2020, 61: 238-250.

[54] Mcclune CJ, Liu JC, Wick C, De La Peña R, Lange BM, Fordyce PM, Sattely ES. Discovery of foto1 and taxol genes enables biosynthesis of baccatin iii. Nature. 2025, 6438071582-592.

[55] Ming R, Xie X, Ling W, Wei Y, Chen J, Yao S, Tan Y, Li L, Huang R, Huang D, Xiao J. Integration of metabolomics and transcriptomics unravels the identification of tps gene family and functional characterization of a sesquiterpenoid synthesis gene in curcuma kwangsiensis. Front. Plant Sci. 2025, 16: 1703946

[56] Oh J, Karadeniz F, Lee J, Seo Y, Kong C. Ligustrum japonicum fructus induces anti-adipogenetic and pro-osteoblastogenic activities in human bone marrow-derived mensencymal stem cells (p06-016-19). Curr Dev Nutr. 2019, 3nzz31-nzz36.

[57] Pan C, Dai G, Zhang H, Zhang C, Meng Q, Xu L, Xu N, Zhang Y, Tan Q, Wang X, Zhang Z. Salidroside ameliorates orthopedic surgery-induced cognitive dysfunction by activating adenosine 5’-monophosphate-activated protein kinase signaling in mice. Eur J Pharmacol. 2022, 929: 175148.

[58] Qiao Z, Tang J, Wu W, Tang J, Liu M. Acteoside inhibits inflammatory response via jak/stat signaling pathway in osteoarthritic rats. BMC Complement Altern Med. 2019, 19(1): 264.

[59] Qin X, Wei Q, An R, Yang Y, Cai M, Han X, Mao H, Gao X. Regulation of bone and fat balance by fructus ligustri lucidi in ovariectomized mice. Pharm Biol. 2023, 611391-403.

[60] Reed J, Orme A, El-Demerdash A, Owen C, Martin LBB, Misra RC, Kikuchi S, Rejzek M, Martin AC, Harkess A, Leebens-Mack J, Louveau T, Stephenson MJ, Osbourn A. Elucidation of the pathway for biosynthesis of saponin adjuvants from the soapbark tree. Science. 2023, 37966381252-1264.

[61] Romsuk J, Yasumoto S, Fukushima EO, Miura K, Muranaka T, Seki H. High-yield bioactive triterpenoid production by heterologous expression in nicotiana benthamiana using the tsukuba system. Front Plant Sci. 2022, 13: 991909.

[62] Romsuk J, Srisawat P, Robertlee J, Yasumoto S, Miura K, Muranaka T, Seki H. Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltrated nicotiana benthamiana leaves. Plant Biotechnol (Tokyo Japan). 2024, 41(3): 277-288.

[63] Seo HL, Baek SY, Lee EH, Lee J, Lee S, Kim K, Jang MH, Park M, Kim J, Kim K, Lee HS, Ahn S, Lee JR, Park SJ, Kim SC, Kim YW. Liqustri lucidi fructus inhibits hepatic injury and functions as an antioxidant by activation of amp-activated protein kinase in vivo and in vitro. Chem Biol Interact. 2017, 262: 57-68.

[64] Shi Y, Wang D, Li R, Huang L, Dai Z, Zhang X. Engineering yeast subcellular compartments for increased production of the lipophilic natural products ginsenosides. Metab Eng. 2021, 67: 104-111.

[65] Song Y, Prather KLJ. Strategies in engineering sustainable biochemical synthesis through microbial systems. Curr Opin Chem Biol. 2024, 81: 102493.

[66] Song X, Li C, Zeng Y, Wu H, Huang Z, Zhang J, Hong R, Chen X, Wang L, Hu X, Su W, Li Y, He Z. Immunomodulatory effects of crude phenylethanoid glycosides from ligustrum purpurascens. J Ethnopharmacol. 2012, 1443584-591.

[67] Su H, Yao YQ, Li ZY, Zhang HY, Wu Q, Shi JS, Li XY. Mechanism of ligustri lucidi fructus on hepatic injury in mice induced by ccl4. West China J Pharm Sci. 2017, 320147-48

[68] Tan HY. Pharmacognostical study on the 6 species medical plants of the genus Ligustrum. 2020, Shandong, Shandong University of Traditional Chinese Medicine

[69] Tan HY, Chen WH, Sun XL, Su Y, Guo N, Xu LC. Research progress on the chemical constituents of main medicinal plants in ligustrum. J Chin Med Mater. 2020, 43(03): 750-757

[70] Temby M, Boye TL, Hoang J, Nielsen OH, Gubatan J. Kinase signaling in colitis-associated colon cancer and inflammatory bowel disease. Biomolecules. 2023, 13(11): 1620.

[71] Tian G, Chen J, Luo Y, Yang J, Gao T, Shi J. Ethanol extract of ligustrum lucidum ait. Leaves suppressed hepatocellular carcinoma in vitro and in vivo. Cancer Cell Int. 2019, 19: 246.

[72] Torrens-Spence MP, Pluskal T, Li F, Carballo V, Weng J. Complete pathway elucidation and heterologous reconstitution of rhodiola salidroside biosynthesis. Mol Plant. 2018, 111205-217.

[73] Vyas P, Srivastava P, Srivastava G, Kumar A, Garg A, Ghosh CHR, S (2025) Ugt73fb1 contributes to scaffold-selective biosynthesis of triterpenoid glucosyl esters in saponin-rich bark of arjuna tree. Plant J 122 (1):e70128

[74] Wang L, Ma R, Guo Y, Sun J, Liu H, Zhu R, Liu C, Li J, Li L, Chen B, Sun L, Tang J, Zhao D, Mo F, Niu J, Jiang G, Fu M, Bromme D, Zhang D, Gao S. Antioxidant effect of fructus ligustri lucidi aqueous extract in ovariectomized rats is mediated through nox4-ros-nf-kappab pathway. Front Pharmacol. 2017, 8: 266.

[75] Wang R, Gao J, Li M, Wu X, Shen C, Wu J, Li Y, Jin Y, Qi Z, Liang Z. Characterization of the complete chloroplast genome of chinese privet ligustrum lucidum (oleaceae). Mitochondrial DNA Part B-Resour. 2018, 32862-863.

[76] Wang Q, Lin J, Li C, Lin M, Zhang Q, Zhang X, Yao K. Traditional chinese medicine method of tonifying kidney for hypertension: clinical evidence and molecular mechanisms. Front Cardiovasc Med. 2022, 9: 1038480.

[77] Wang H, Wang Z, Chen K, Yao M, Zhang M, Wang R, Zhang J, Agren H, Li F, Li J, Qiao X, Ye M. Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of leguminosae plants. Nat Commun. 2023, 1416658.

[78] Wu L, Georgiev MI, Cao H, Nahar L, El-Seedi HR, Sarker SD, Xiao J, Lu B. Therapeutic potential of phenylethanoid glycosides: a systematic review. Med Res Rev. 2020, 4062605-2649.

[79] Xiong Y, Wang Y, Xiong Y, Teng L. Protective effect of salidroside on hypoxia-related liver oxidative stress and inflammation via nrf2 and jak2/stat3 signaling pathways. Food Sci Nutr. 2021, 995060-5069.

[80] Xu D, Lyu Y, Chen X, Zhu X, Feng J, Xu Y. Fructus ligustri lucidi ethanol extract inhibits osteoclastogenesis in raw264.7 cells via the rankl signaling pathway. Mol Med Rep. 2016, 14(5): 4767-4774.

[81] Xu B, Huang J, Peng G, Cao W, Liu Z, Chen Y, Yao J, Wang Y, Li J, Zhang G, Chen S, Huang S. Total biosynthesis of the medicinal triterpenoid saponin astragalosides. Nat Plants. 2024, 10(11): 1826-1837.

[82] Xue QP, Hu Y, Guo ZJ, Ma HT, Zhang LJ. Study on the influence of rice wine type on the quality of wine ligustrilucidi fructus based on uplc combined with pca method. Asia Pac Tradit Med. 2024, 20(08): 54-59

[83] Yan B, Zhou L, Wang C, Wang R, Yan L, Yu L, Liu F, Du W, Yu G, Yuan Q, Tong P, Shan L, Efferth T. Intra-articular injection of fructus ligustri lucidi extract attenuates pain behavior and cartilage degeneration in mono-iodoacetate induced osteoarthritic rats. Front Pharmacol. 2018, 9: 1360.

[84] Yang R, Chu X, Sun L, Kang Z, Ji M, Yu Y, Liu Y, He Z, Gao N. Hypolipidemic activity and mechanisms of the total phenylpropanoid glycosides from ligustrum robustum (roxb.) Blume by ampk-srebp-1c pathway in hamsters fed a high-fat diet. Phytother Res. 2018, 32(4): 715-722.

[85] Yang Y, Wu Y, Zhuang Y, Liu T. Discovery of glycosyltransferases involved in the biosynthesis of ligupurpuroside b. Org Lett. 2021, 23207851-7854.

[86] Yang X, Li Y, Guo J, Wang J, Li S, Yang Z, Niu P, Jiang Y, Song M, Hai Y. Role and mechanism of botanical drugs and their metabolites in osteoporosis: new strategies for clinical application. Front Pharmacol. 2025, 16: 1530194.

[87] Ye M, Kim M, Bae H. Neuro-protective effects of ligustri fructus by suppression of oxidative stress in mouse model of parkinson’s disease. Orient Pharm Exp Med. 2016, 16(2): 123-129.

[88] Zeng W, Wang H, Chen J, Hu M, Wang X, Chen J, Zhou J. Engineering Escherichia coli for efficient de novo synthesis of salidroside. J Agric Food Chem. 2024, 725128369-28377.

[89] Zhang Y, Wiese L, Fang H, Alseekh S, De Souza P, Scossa L, Molloy F, Christmann J, Fernie M. Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis. Mol Plant. 2023, 16(12): 1951-1961.

[90] Zhao Y, Fan J, Wang C, Feng X, Li C. Enhancing oleanolic acid production in engineered saccharomyces cerevisiae. Bioresour Technol. 2018, 257: 339-343.

[91] Zhou P, Dang J, Jiang Z, Dai S, Qu C, Wu Q. Transcriptome and metabolome analysis revealed the dynamic change of bioactive compounds of fructus ligustri lucidi. BMC Plant Biol. 2024, 24(1): 489.

[92] Zhu Y, Yan X, Li W, Qiao J, Zhao G. Modular metabolic engineering of saccharomyces cerevisiae for enhanced production of ursolic acid. J Agric Food Chem. 2025, 7363580-3590.

[93] Zuo L, Ge X, Qiao Q, Lv H, Xu Z, Xu S, Li L (2025) Metabolomics analysis on different parts of ligustrum lucidum ait based on uplc-q-tof-ms. J. AOAC Int 108(5):786-95

Funding

National Natural Science Foundation of China(82174400)

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