Sustainable bioconversion of excess grape must into polyhydroxyalkanoates by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034

Keywords

Cupriavidus necator / Hydrogenophaga pseudoflava / Polyhydroxyalkanoates / Grape must / Fed-batch / Bioplastics

References

[1] Anderson K. The emergence of lower-alcohol beverages: the case of beer. J Wine Econ. 2023, 18: 66-86.

[2] Anderson K, Pinilla V (2017) Annual database of global wine markets, 1835 to 2016. University of Adelaide’s Wine Economics Research Centre

[3] Anjum A, Zuber M, Zia KM, Noreen A, Anjum MN, Tabasum S. Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: a review of recent advancements. Int J Biol Macromol. 2016.

[4] Auling G, Reh M, Lee CM, Schlegel HG. Pseudomonas pseudoflava, a new species of hydrogen-oxidizing bacteria: its differentiation from Pseudomonas flava and other yellow-pigmented, gram-negative, hydrogen-oxidizing species. Int J Syst Evol Microbiol. 1978, 28: 82-95

[5] Barata A, Pais A, Malfeito-Ferreira M, Loureiro V. Influence of sour rotten grapes on the chemical composition and quality of grape must and wine. Eur Food Res Technol. 2011, 233: 183-194.

[6] Basaglia M, D’ambra M, Piubello G, Zanconato V, Favaro L, Casella S. Agro-food residues and bioethanol potential: a study for a specific area. Processes. 2021, 9: 1-15.

[7] Bellini S, Tommasi T, Fino D. Poly(3-hydroxybutyrate) biosynthesis by Cupriavidus necator: a review on waste substrates utilization for a circular economy approach. Bioresour Technol Rep. 2022.

[8] Bellini S, Demichelis F, Tommasi T, Tarraran L, Fino D. Integrated biorefinery strategy for poly(3-hydroxybutyrate) accumulation in Cupriavidus necator DSM 545 using a sugar rich syrup from cereal waste and acetate from gas fermentation. J Environ Chem Eng. 2024.

[9] Blunt W, Shah P, Vasquez V, Ye M, Doyle C, Liu Y, Saeidlou S, Monteil-Rivera F. Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C5 and C6 sugars obtained from hardwood hydrolysis. N Biotechnol. 2023, 77: 40-49.

[10] Braunegg G, Sonnleimer B, Lafferty RM. A rapid gas chromatographic method for the determination of polyhydroxybutyric acid in microbial biomass. Eur J Appl Microbiol Biotechnol. 1978, 6: 29-37.

[11] Brojanigo S, Alvarado-Morales M, Basaglia M, Casella S, Favaro L, Angelidaki I. Innovative co-production of polyhydroxyalkanoates and methane from broken rice. Sci Total Environ. 2022.

[12] Brojanigo S, Parro E, Cazzorla T, Favaro L, Basaglia M, Casella S. Conversion of starchy waste streams into polyhydroxyalkanoates using Cupriavidus necator DSM 545. Polymers. 2020, 12: 1496.

[13] Caminiti V, Gupte AP, Favaro L, Casella S, Basaglia M. Valorization of prosecco wine lees for sustainable polyhydroxyalkanoates production by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034. N Biotechnol. 2025, 8873-82.

[14] Coppo E, Marchese A. Antibacterial activity of polyphenols. Curr Pharm Biotechnol. 2014, 15: 380-390.

[15] Costa P, Basaglia M, Casella S, Favaro L. Polyhydroxyalkanoate production from fruit and vegetable waste processing. Polymers. 2022, 14(24): 5529.

[16] Costa P, Basaglia M, Casella S, Kennes C, Favaro L, Carmen Veiga M. Autotrophic production of polyhydroxyalkanoates using acidogenic-derived H₂ and CO₂ from fruit waste. Bioresour Technol. 2023.

[17] Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012, 232174-181.

[18] Dimou C, Kopsahelis N, Papadaki A, Papanikolaou S, Kookos IK, Mandala I, Koutinas AA. Wine lees valorization: biorefinery development including production of a generic fermentation feedstock employed for poly(3-hydroxybutyrate) synthesis. Food Res Int. 2015, 73: 81-87.

[19] El Rayess Y, Castro-Muñoz R, Cassano A. Current advances in membrane processing of wines: a comprehensive review. Trends Food Sci Technol. 2024, 147: 104453.

[20] Favaro L, Basaglia M, Casella S. Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review. Biofuels Bioprod Biorefin. 2019, 13(1): 208-227.

[21] Favaro L, Casella S, Parro E, Franzosi G, Rodighiero V, Basaglia M. Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034. New Biotechnol. 2025, 87: 72-81.

[22] Ganesh Saratale R, Cho SK, Dattatraya Saratale G, Kadam AA, Ghodake GS, Kumar M, Naresh Bharagava R, Kumar G, Su Kim D, Mulla SI, Seung Shin H. A comprehensive overview and recent advances on polyhydroxyalkanoates (PHA) production using various organic waste streams. Bioresour Technol. 2021, 325: 124685.

[23] Geyer R, Jambeck JR, Law KL. Production, use, and fate of all plastics ever made. Sci Adv. 2017, 3e1700782.

[24] Giovinazzo G, Grieco F. Functional properties of grape and wine polyphenols. Plant Foods Hum Nutr. 2015, 70454-462.

[25] Gupte AP, Basaglia M, Casella S, Favaro L. Rice waste streams as a promising source of biofuels: feedstocks, biotechnologies and future perspectives. Renew Sustain Energy Rev. 2022, 131208-227.

[26] Gupte AP, Di Vita N, Myburgh MW, Cripwell RA, Basaglia M, van Zyl WH, Viljoen-Bloom M, Casella S, Favaro L. Consolidated bioprocessing of the organic fraction of municipal solid waste into bioethanol. Energy Convers Manag. 2024, 302: 118105.

[27] Jambeck JR, Geyer R, Wilcox C, Siegler TR, Perryman M, Andrady A, Narayan R, Kara Lavende Law K. Plastic waste inputs from land into the ocean. Science. 2015, 3476223768-771.

[28] Koller M, Hesse P, Bona R, Kutschera C, Atlić A, Braunegg G. Potential of various archae- and eubacterial strains as industrial polyhydroxyalkanoate producers from whey. Macromol Biosci. 2007, 7: 218-226.

[29] Koller M, Mukherjee A. A new wave of industrialization of PHA biopolyesters. Bioengineering. 2022, 9274.

[30] Kovalcik A, Pernicova I, Obruca S, Szotkowski M, Enev V, Kalina M, Marova I. Grape winery waste as a promising feedstock for the production of polyhydroxyalkanoates and other value-added products. Food Bioprod Process. 2020, 124: 1-10.

[31] Liu CC, Zhang LL, An J, Chen B, Yang H. Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms. Lett Appl Microbiol. 2016, 62: 9-15.

[32] Lopar M, Špoljarić IV, Cepanec N, Koller M, Braunegg G, Horvat P. Study of metabolic network of Cupriavidus necator DSM 545 growing on glycerol by applying elementary flux modes and yield space analysis. J Ind Microbiol Biotechnol. 2014, 4: 913-930.

[33] Notarnicola B, Tassielli G, Renzulli PA. Environmental and technical improvement of a grape must concentration system via a life cycle approach. J Clean Prod. 2015, 89: 87-98.

[34] Nygaard D, Yashchuk O, Noseda DG, Araoz B, Hermida ÉB. Improved fermentation strategies in a bioreactor for enhancing poly(3-hydroxybutyrate) (PHB) production by wild type Cupriavidus necator from fructose. Heliyon. 2021.

[35] Obruca S, Sedlacek P, Koller M. The underexplored role of diverse stress factors in microbial biopolymer synthesis. Bioresour Technol. 2021, 326: 124767.

[36] Obruca S, Sedlacek P, Koller M, Kucera D, Pernicova I. Involvement of polyhydroxyalkanoates in stress resistance of microbial cells: biotechnological consequences and applications. Biotechnol Adv. 2018, 363856-870.

[37] OIV (2025) State of the World Vine and Wine Sector in 2024. International Organisation of Vine and Wine. https://www.oiv.int/sites/default/files/2025-04/OIV-State_of_the_World_Vine-and-Wine-Sector-in-2024.pdf

[38] Omar S, Rayes A, Eqaab A, Voß I, Steinbüchel A. Optimization of cell growth and poly (3-hydroxybutyrate) accumulation on date syrup by a Bacillus megaterium strain. Biotechnol Lett. 2001, 23: 1119-1123.

[39] Pan W, Perrotta JA, Stipanovic AJ, Nomura CT, Nakas JP. Production of polyhydroxyalkanoates by Burkholderia cepacia ATCC 17759 using a detoxified sugar maple hemicellulosic hydrolysate. J Ind Microbiol Biotechnol. 2012, 39: 459-469.

[40] Povolo S, Toffano P, Basaglia M, Casella S. Polyhydroxyalkanoates production by engineered Cupriavidus necator from waste material containing lactose. Bioresour Technol. 2010, 101: 7902-7907.

[41] Povolo S, Romanelli MG, Fontana F, Basaglia M, Casella S. Production of polyhydroxyalkanoates from fatty wastes. J Polym Environ. 2012, 20: 944-949.

[42] Povolo S, Romanelli MG, Basaglia M, Ilieva VI, Corti A, Morelli A, Chiellini E, Casella S. Polyhydroxyalkanoate biosynthesis by Hydrogenophaga pseudoflava DSM 1034 from structurally unrelated carbon sources. New Biotechnol. 2013, 30: 629-634.

[43] Ramsay BA, Lomaliza K, Chavarie C, Dube B, Bataille P, Ramsay JA. Production of poly-(P-hydroxybutyric-co-3-hydroxyvaleric) acids. Appl Environ Microbiol. 1990, 56: 2093-2098.

[44] Richardson JB, Chase JK. Transfer of macronutrients, micronutrients, and toxic elements from soil to grapes to white wines in uncontaminated vineyards. Int J Environ Res Public Health. 2021, 18: 13271.

[45] Rodríguez G. JE, Brojanigo S, Basaglia M, Favaro L, Casella S. Efficient production of polyhydroxybutyrate from slaughterhouse waste using a recombinant strain of Cupriavidus necator DSM 545. Sci Total Environ. 2021.

[46] Sabapathy PC, Devaraj S, Meixner K, Anburajan P, Kathirvel P, Ravikumar Y, Zabed HM, Qi X. Recent developments in Polyhydroxyalkanoates (PHAs) production—a review. Bioresour Technol. 2020, 306123132.

[47] Santimano MC, Prabhu NN, Garg S. PHA production using low-cost agro-industrial wastes by Bacillus sp. strain COL1/A6. Res J Microbiol. 2009, 489-96.

[48] Schlegel HG, Gottschalk G. Verwertung yon Glucose durch eine Mutante von Hydrogenomonas H16. Biochem Z. 1965, 841: 249-259

[49] Sirohi R, Prakash Pandey J, Kumar Gaur V, Gnansounou E, Sindhu R. Critical overview of biomass feedstocks as sustainable substrates for the production of polyhydroxybutyrate (PHB). Bioresour Technol. 2020.

[50] Wang J, Huo S, Zhang Y, Liu Y, Fan W. Effect of different pre-fermentation treatments on polyphenols, color, and volatile compounds of three wine varieties. Food Sci Biotechnol. 2016, 25: 735-743.

Funding

Università degli Studi di Padova(BIRD234877/23)

National Ph.D. Programme in Scientific, Technological and Social Methods Enabling Circular Economy. (National Ph.D. Programme in Scientific, Technological and Social Methods Enabling Circular Economy.)

RIGHTS & PERMISSIONS

The Author(s)