Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil

Abstract

This study explored a novel laccase production process utilizing perennial biomass and the aqueous phase from bio-oil. Experimental and techno-economic modeling were coupled to gain insight into and optimize the solid-state fermentation conditions for minimizing the laccase selling price. At relatively small scales, processing only 230 Mg of prairie biomass per year, a 5-year return on investment required a minimum laccase selling price of $0.05/kU, assuming a 10% discount rate. The associated sensitivity analysis showed the minimum laccase selling price to be most sensitive to parameters affecting laccase output from the plant, which included the number of batches produced per year and laccase recovery assumptions. Limitations and future outlooks were provided, emphasizing the need for further process optimization to minimize fermentation time and downstream losses.

References

[1] Bakratsas G, Antoniadis K, Athanasiou PE, Katapodis P, Stamatis H (2024) Laccase and biomass production via submerged cultivation of pleurotus ostreatus using wine lees. Biomass 4(1). https://doi.org/10.3390/biomass4010001

[2] Baltierra-TrejoE, Márquez-BenavidesL, Sánchez-YáñezJM. Inconsistencies and ambiguities in calculating enzyme activity: the case of laccase. J Microbiol Methods, 2015, 119: 126-131.

[3] BrugnariT, BragaDM, dos SantosCSA, TorresBHC, ModkovskiTA, HaminiukCWI, MacielGM. Laccases as green and versatile biocatalysts: from lab to enzyme market—an overview. Bioresources Bioprocess, 2021, 81131.

[4] Bryjak J, Rekuć A (2010) Effective Purification of Cerrena unicolor Laccase Using Microfiltration, Ultrafiltration and Acetone Precipitation. Appl Biochem Biotechnol. 160(8):2219–35. https://doi.org/10.1007/s12010-009-8791-9

[5] U.S. Department of Energy (2023) 2023 Billion-Ton Report: An Assessment of U.S. Renewable Carbon Resources. https://www.energy.gov/sites/default/files/2024-03/beto-2023-billion-ton-report_2.pdf

[6] Diaz R, Alonso S, Sánchez C, Tomasini A, Bibbins M, Diaz-Godínez G (2010) Characterization of the growth and laccase activity of strains of pleurotus ostreatus in submerged fermentation. BioResources 6. https://doi.org/10.15376/biores.6.1.282-290

[7] DwivediUN, SinghP, PandeyVP, KumarA. Structure–function relationship among bacterial, fungal and plant laccases. J Mol Catal B: Enzymatic, 2011, 68(2): 117-128.

[8] El-NaggarA, LeeSS, RinklebeJ, FarooqM, SongH, SarmahAK, ZimmermanAR, AhmadM, ShaheenSM, OkYS. Biochar application to low fertility soils: A review of current status, and future prospects. Geoderma, 2019, 337: 536-554.

[9] Gao X, Tang X, Zhao K, Balan V, Zhu Q (2021) Biogas production from anaerobic co-digestion of spent mushroom substrate with different livestock manure. Energies 14(3). https://doi.org/10.3390/en14030570

[10] HafidHS, BaharuddinAS, MokhtarMN, OmarFN, MohammedMAP, WakisakaM. Enhanced laccase production for oil palm biomass delignification using biological pretreatment and its Estimation at biorefinary scale. Biomass Bioenergy, 2021, 144105904.

[11] IghaloJO, OmoarukheFO, OjukwuVE, IwuozorKO, IgwegbeCA. Cost of adsorbent Preparation and usage in wastewater treatment: A review. Clean Chem Eng, 2022, 3100042.

[12] Janusz G, Pawlik A, Świderska-Burek U, Polak J, Sulej J, Jarosz-Wilkołazka A, Paszczyński A (2020) Laccase properties, physiological functions, and evolution. Int J Mol Sci 21(3). https://doi.org/10.3390/ijms21030966

[13] KordbachehF, JarchowM, EnglishL, LiebmanM. Productivity and diversity of annually harvested reconstructed prairie communities. J Appl Ecol, 2019, 56(2): 330-342.

[14] Lachos-Perez D, Martins-Vieira JC, Missau J, Anshu K, Siakpebru OK, Thengane SK, Morais ARC, Tanabe EH, Bertuol DA (2023) Review on biomass pyrolysis with a focus on bio-oil upgrading techniques. Analytica 4(2) Article 2. https://doi.org/10.3390/analytica4020015

[15] LiangY, ZhaoX, ChiZ, RoverM, JohnstonP, BrownR, JarboeL, WenZ. Utilization of acetic acid-rich pyrolytic bio-oil by microalga chlamydomonas reinhardtii: reducing bio-oil toxicity and enhancing algal toxicity tolerance. Bioresour Technol, 2013, 133: 500-506.

[16] LinL, ShahA, KeenerH, LiY. Techno-economic analyses of solid-state anaerobic digestion and composting of yard trimmings. Waste Manag, 2019, 85: 405-416.

[17] LouZ, SunY, BianS, Ali BaigS, HuB, XuX. Nutrient conservation during spent mushroom compost application using spent mushroom substrate derived Biochar. Chemosphere, 2017, 169: 23-31.

[18] MananMA, WebbC. Newly designed multi-stacked circular tray solid-state bioreactor: analysis of a distributed parameter gas balance during solid-state fermentation with influence of variable initial moisture content arrangements. Bioresources Bioprocess, 2020, 7116.

[19] Mayolo-DeloisaK, González-GonzálezM, Rito-PalomaresM. Laccases in food industry: bioprocessing, potential industrial and biotechnological applications. Front Bioeng Biotechnol, 2020.

[20] MazumderS, BasuSK, MukherjeeM. Laccase production in solid-state and submerged fermentation by pleurotus ostreatus. Eng Life Sci, 2009, 9(1): 45-52.

[21] OlafasakinO, AudiaEM, Mba-WrightM, TyndallJC, SchulteLA. Techno-economic and life cycle analysis of renewable natural gas derived from anaerobic digestion of grassy biomass: A US corn belt watershed case study. GCB Bioenergy, 2024, 166e13164.

[22] PatelAK, SinghaniaRR, PalA, ChenC-W, PandeyA, DongC-D. Advances on tailored Biochar for bioremediation of antibiotics, pesticides and polycyclic aromatic hydrocarbon pollutants from aqueous and solid phases. Sci Total Environ, 2022, 817153054.

[23] PitolLO, BizA, MallmannE, KriegerN, MitchellDA. Production of pectinases by solid-state fermentation in a pilot-scale packed-bed bioreactor. Chem Eng J, 2016, 283: 1009-1018.

[24] PollardAS, RoverMR, BrownRC. Characterization of bio-oil recovered as stage fractions with unique chemical and physical properties. J Anal Appl Pyrol, 2012, 93: 129-138.

[25] Rahic E (2024) Bioprocessing strategies to valorize prairie biomass [Ph.D., Iowa State University]. https://www.proquest.com/docview/3116908135/abstract/CA35A94D097047FEPQ/1

[26] RahicE, HessCJ, BrownRC, WenZ. Enhanced anaerobic digestion of prairie biomass through alkaline-based hydroxycinnamic acid extraction. Bioresource Technol Rep, 2024, 27101896.

[27] SchulteLA, NiemiJ, HelmersMJ, LiebmanM, ArbuckleJG, JamesDE, KolkaRK, O’NealME, TomerMD, TyndallJC, AsbjornsenH, DrobneyP, NealJ, Van RyswykG, WitteC. Prairie strips improve biodiversity and the delivery of multiple ecosystem services from corn–soybean croplands. Proc Natl Acad Sci, 2017, 114(42): 11247-11252.

[28] Téllez-TéllezM, FernándezFJ, Montiel-GonzálezAM, SánchezC, Díaz-GodínezG. Growth and laccase production by pleurotus ostreatus in submerged and solid-state fermentation. Appl Microbiol Biotechnol, 2008, 81(4): 675-679.

[29] TinocoR, AcevedoA, GalindoE, Serrano-CarreónL. Increasing pleurotus ostreatus laccase production by culture medium optimization and copper/lignin synergistic induction. J Ind Microbiol Biotechnol, 2011, 38(4): 531-540.

[30] Tlecuitl-BeristainS, SánchezC, LoeraO, RobsonGD, Díaz-GodínezG. Laccases of pleurotus ostreatus observed at different phases of its growth in submerged fermentation: production of a novel laccase isoform. Mycol Res, 2008, 112(9): 1080-1084.

[31] van der SpekM, RamirezA, FaaijA. Challenges and uncertainties of ex ante techno-economic analysis of low TRL CO2 capture technology: lessons from a case study of an NGCC with exhaust gas recycle and electric swing adsorption. Appl Energy, 2017, 208: 920-934.

[32] Vasco-Correa J, Shah A (2019) Techno-Economic bottlenecks of the fungal pretreatment of lignocellulosic biomass. Fermentation 5(2) Article 2. https://doi.org/10.3390/fermentation5020030

[33] Vasilakis G, Rigos E-M, Giannakis N, Diamantopoulou P, Papanikolaou S (2023) Spent mushroom substrate hydrolysis and utilization as potential alternative feedstock for anaerobic Co-Digestion. Microorganisms 11(2). https://doi.org/10.3390/microorganisms11020532

[34] Verret J, Qiao R Barghout RA (2020) Foundations of chemical and biological engineering I., BCcampus. https://pressbooks.bccampus.ca/chbe220/

[35] WildK, RahicE, SchulteL, Mba WrightM. Techno-economic and environmental assessment of converting mixed prairie to renewable natural gas with co-product hydroxycinnamic acid. Biofuels Bioprod Biorefin, 2025, 19(2): 288-304.

[36] WilliamsRJr. Six-Tenths factor aids in approximating costs. Chem Eng, 1947, 54: 124-125

[37] YangM, RosentraterKA. Techno-economic analysis of the production process of structural bio-adhesive derived from glycerol. J Clean Prod, 2019, 228: 388-398.

[38] Zaccaria S, Boff NA, Bettin F, Dillon AJP (2019) Use of micro- and ultrafiltration membranes for concentration of laccase-rich enzymatic extract of Pleurotus sajor-caju PS-2001 and application in dye decolorization. Chem Pap 73(12):3085–94. https://doi.org/10.1007/s11696-019-00845-3

[39] Zerva A, Simić S, Topakas E, Nikodinovic-Runic J (2019) Applications of microbial laccases: patent review of the past decade (2009–2019). Catalysts 9(12). https://doi.org/10.3390/catal9121023

[40] ZhangY, LiangY, LiS, YuanY, ZhangD, WuY, XieH, BrindhadeviK, PugazhendhiA, XiaC. A review of biomass pyrolysis gas: forming mechanisms, influencing parameters, and product application upgrades. Fuel, 2023, 347128461.

[41] ZhaoL. Improvement of laccase production by pleurotus ostreatus by means of agroindustrial waste and fermentation kinetics. Mycosphere, 2017, 8(1): 147-161.

[42] ZhaoX, DavisK, BrownR, JarboeL, WenZ. Alkaline treatment for detoxification of acetic acid-rich pyrolytic bio-oil for microalgae fermentation: effects of alkaline species and the detoxification mechanisms. Biomass Bioenergy, 2015, 80: 203-212.

[43] ZhaoX, JarboeL, WenZ. Utilization of pyrolytic substrate by microalga chlamydomonas reinhardtii: cell membrane property change as a response of the substrate toxicity. Appl Microbiol Biotechnol, 2016, 100(9): 4241-4251.

[44] ZhouH, BrownRC, WenZ. Anaerobic digestion of aqueous phase from pyrolysis of biomass: reducing toxicity and improving microbial tolerance. Bioresour Technol, 2019, 292121976.

[45] ZhouH, BrownRC, WenZ. Biochar as an additive in anaerobic digestion of municipal sludge: Biochar properties and their effects on the digestion performance. ACS Sustain Chem Eng, 2020, 8(16): 6391-6401.

Funding

National Institute of Food and Agriculture(2020-68012-31824)

RIGHTS & PERMISSIONS

The Author(s)