Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis

Abstract

Astaxanthin biosynthesis in Haematococcus pluvialis is driven by energy. However, the effect of the flagella-mediated energy-consuming movement process on astaxanthin accumulation has not been well studied. In this study, the profiles of astaxanthin and NADPH contents in combination with the photosynthetic parameters with or without flagella enabled by pH shock were characterized. The results demonstrated that there was no significant alteration in cell morphology, with the exception of the loss of flagella observed in the pH shock treatment group. In contrast, the astaxanthin content in the flagella removal groups was 62.9%, 62.8% and 91.1% higher than that of the control at 4, 8 and 12 h, respectively. Simultaneously, the increased Y(II) and decreased Y(NO) suggest that cells lacking the flagellar movement process may allocate more energy towards astaxanthin biosynthesis. This finding was verified by NADPH analysis, which revealed higher levels in flagella removal cells. These results provide preliminary insights into the underlying mechanism of astaxanthin accumulation enabled by energy reassignment in movement-lacking cells.

Keywords

Haematococcus pluvialis / Astaxanthin / Flagella / Energy flow / pH-shock

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Funding

National Key R&D Program of China(2022YFC3401803)

Hundred Talents Program of the Chinese Academy of Sciences(E3J56201)

Science and Technology Partnership Program, Ministry of Science and Technology of China(KY202001017)

Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-IJCP-001; TSBICIP-CXRC-027)