Co-immobilization of amine dehydrogenase and glucose dehydrogenase for the biosynthesis of (S)-2-aminobutan-1-ol in continuous flow

Abstract

Reductive amination by amine dehydrogenases is a green and sustainable process that produces only water as the by-product. In this study, a continuous flow process was designed utilizing a packed bed reactor filled with co-immobilized amine dehydrogenase wh84 and glucose dehydrogenase for the highly efficient biocatalytic synthesis of chiral amino alcohols. The immobilized amine dehydrogenase wh84 exhibited better thermo-, pH and solvent stability with high activity recovery. (S)-2-aminobutan-1-ol was produced in up to 99% conversion and 99% ee in the continuous flow processes, and the space-time yields were up to 124.5 g L^-1 d^-1. The continuous reactions were also extended to 48 h affording up to 91.8% average conversions. This study showcased the important potential to sustainable production of chiral amino alcohols in continuous flow processes.

Keywords

Continuous flow / Asymmetric reductive amination / Amine dehydrogenases / Co-immobilization / Packed bed reactor

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Funding

National Key Research and Development Program of China(2021YFA0910400)

National Natural Science Foundation of China(32171462)

Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-CXRC-040)

Natural Science Foundation of Tianjin(21JCJQJC00110)