Enhancing the biosynthesis of taxadien-5α-yl-acetate in Escherichia coli by combinatorial metabolic engineering approaches

Abstract

We used E. coli BL21 (DE3) to de novo synthesize T5OAc.

Increasing precursor and relieving metabolic stress to enhance T5OAc production.

The modular metabolic engineering strategy achieved the balance of metabolic flux.

Keywords

Escherichia coli / Methylerythritol phosphate pathway / Taxadien-5α-yl-acetate / Taxadien-5α-ol O-acetyltransferase / Multivariate-modular metabolic engineering

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Funding

National Key Research and Development Program of China(2019YFA0905000)

National Natural Science Foundation of China(21871085)

Fundamental Research Funds for the Central Universities(222201714026)