Recent advances in the biosynthesis and industrial biotechnology of Gamma-amino butyric acid

Abstract

GABA (Gamma-aminobutyric acid), a crucial neurotransmitter in the central nervous system, has gained significant attention in recent years due to its extensive benefits for human health. The review focused on recent advances in the biosynthesis and production of GABA. To begin with, the investigation evaluates GABA-producing strains and metabolic pathways, focusing on microbial sources such as Lactic Acid Bacteria, Escherichia coli, and Corynebacterium glutamicum. The metabolic pathways of GABA are elaborated upon, including the GABA shunt and critical enzymes involved in its synthesis. Next, strategies to enhance microbial GABA production are discussed, including optimization of fermentation factors, different fermentation methods such as co-culture strategy and two-step fermentation, and modification of the GABA metabolic pathway. The review also explores methods for determining glutamate (Glu) and GABA levels, emphasizing the importance of accurate quantification. Furthermore, a comprehensive market analysis and prospects are provided, highlighting current trends, potential applications, and challenges in the GABA industry. Overall, this review serves as a valuable resource for researchers and industrialists working on GABA advancements, focusing on its efficient synthesis processes and various applications, and providing novel ideas and approaches to improve GABA yield and quality.

Keywords

Gamma-aminobutyric acid / Biosynthesis / Microbial production / Fermentation optimization / Metabolic pathways

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Funding

Key Technologies Research and Development Program(2019YFA0905700); National Natural Science Foundation of China(21878151); Natural Science Foundation of Jiangsu Province(BK20211535); Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTD2213); “333 Project” of Jiangsu Province(2022)