Intervention mechanism of marine-based chito-oligosaccharide on acute liver injury induced by AFB₁ in rats

Abstract

AFB₁ induced oxidative stress injury and apoptosis of liver.

AFB₁ activated Lama5, Egr1, Cyp2b1, and Gadd45g genes and signaling pathways.

COS attenuated AFB₁-induced hepatotoxicity.

COS regulated Lama5, Egr1, Cyp2b1, and Gadd45g genes and signaling pathways.

Keywords

Aflatoxin B₁ / Chito-oligosaccharide / Oxidative Stress / Apoptosis / RNA-Seq

References

[1] Ahn HY, Cho HD, Cho YS. Anti-oxidant and anti-hyperlipidemic effects of cordycepin-rich Cordyceps militaris in a Sprague-Dawley rat model of alcohol-induced hyperlipidemia and oxidative stress. Bioresour Bioprocess, 2020, 7(1): 33.

[2] Argyriou C, D’Agostino MD, Braverman N. Peroxisome biogenesis disorders. Transl Sci Rare Dis, 2016, 1(2): 111-144.

[3] Conte G, Fontanelli M, Galli F, Cotrozzi L, Pagni L, Pellegrini E. Mycotoxins in feed and food and the role of ozone in their detoxification and degradation: an update. Toxins (basel), 2020, 12(8): 486.

[4] Ding C, Shi X, Guan YL, Li XJ. Deoxynivalenol induces carp neutrophil apoptosis and necroptosis via CYP450s/ROS/PI3K/AKT pathway. Aquaculture, 2021, 545.

[5] Dou C, Zhang B, Han M, Jin X, Sun L, Li T. Anti-tumor activity of polysaccharides extracted from Senecio scandens Buch, -Ham root on hepatocellular carcinoma. Trop J Pharm Res, 2017, 16(1): 43-49.

[6] Fang X, Corrales J, Thornton C, Clerk T, Scheffler BE, Willett KL. Transcriptomic changes in zebrafish embryos and larvae following benzo[a]pyrene exposure. Toxicol Sci, 2015, 146(2): 395-411.

[7] Fang T, Yao Y, Tian G, . Chitosan oligosaccharide attenuates endoplasmic reticulum stress-associated intestinal apoptosis via the Akt/mTOR pathway. Food Funct, 2021, 12(18): 8647-8658.

[8] Farhan M, Wang H, Gaur U, Little PJ, Xu J, Zheng W. FOXO signaling pathways as therapeutic targets in cancer. Int J Biol Sci, 2017, 13(7): 815-827.

[9] Hisamori S, Tabata C, Kadokawa Y, . All-trans-retinoic acid ameliorates carbon tetrachloride-induced liver fibrosis in mice through modulating cytokine production. Liver Int, 2008, 28(9): 1217-1225.

[10] Hua H, Sheng J, Cui Y, . The intervention and mechanism of action for aloin against subchronic aflatoxin B1 induced hepatic injury in rats. Int J Mol Sci, 2021, 22(21): 11620.

[11] Hussain Z, Khan MZ, Khan A, . Residues of aflatoxin B1 in broiler meat: effect of age and dietary aflatoxin B1 levels. Food Chem Toxicol, 2010, 48(12): 3304-3307.

[12] Karadeniz F, Artan M, Kong CS, Kim SK. Chitooligosaccharides protect pancreatic β-cells from hydrogen peroxide-induced deterioration. Carbohyd Polym, 2010, 82(1): 143-147.

[13] Karrer TM, Garmhausen M, Li X, Jansen G. Combining hematoxylin and eosin (H&E) stained images and RNA sequencing (RNA-Seq) data to predict overall survival (OS) in patients with non-small cell lung cancer (NSCLC). J Clin Oncol, 2021, 39(15): 1547-1547.

[14] Kim JA. Peroxisome metabolism in cancer. Cells, 2020, 9(7): 169.

[15] Lan R, Chang Q, An L, Zhao Z. Dietary supplementation with chitosan oligosaccharides alleviates oxidative stress in rats challenged with hydrogen peroxide. Animals (basel), 2019, 10(1): 55.

[16] Larasati YA, Yoneda-Kato N, Nakamae I, Yokoyama T, Meiyanto E, Kato JY. Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth. Sci Rep, 2018, 8(1): 2039.

[17] Lee S, Dong HH. FoxO integration of insulin signaling with glucose and lipid metabolism. J Endocrinol, 2017, 233(2): R67-R79.

[18] Limaye A, Yu RC, Chou CC, Liu JR, Cheng KC. Protective and detoxifying effects conferred by dietary selenium and curcumin against AFB1-mediated toxicity in livestock: a review. Toxins (basel), 2018, 10(1): 25.

[19] Lin CY, Adhikary P, Cheng K. Cellular protein markers, therapeutics, and drug delivery strategies in the treatment of diabetes-associated liver fibrosis. Adv Drug Deliv Rev, 2021, 174: 127-139.

[20] Lu XY, Hu B, Li S, . Integrated analysis of transcriptomics and metabonomics profiles in aflatoxin B1-induced hepatotoxicity in rat. Food Chem Toxicol, 2013, 55: 444-455.

[21] Mahata M, Shinya S, Masaki E, . Production of chitooligosaccharides from Rhizopus oligosporus NRRL2710 cells by chitosanase digestion. Carbohydr Res, 2014, 383: 27-33.

[22] Mohammed J, Tadros G, Michel E. Geraniol protects against cyclophosphamide-induced hepatotoxicity in rats: possible role of MAPK and PPAR-γ signaling pathways. Food Chem Toxicol, 2020, 139.

[23] Naveed M, Phil L, Sohail M, . Chitosan oligosaccharide (COS): an overview. Int J Biol Macromol, 2019, 129: 827-843.

[24] Niki E, Yoshida Y, Saito Y, Noguchi N. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem Biophys Res Commun, 2005, 338(1): 668-676.

[25] Pei CX, Jia N, Wang YL, . Notoginsenoside R1 protects against hypobaric hypoxia-induced high-altitude pulmonary edema by inhibiting apoptosis via ERK1/2-P90rsk-BAD ignaling pathway. Eur J Pharmacol, 2023, 959(15

[26] Possomato-Vieira JS, Khalil RA. Khalil RA. Chapter eleven—Mechanisms of endothelial dysfunction in hypertensive pregnancy and preeclampsia. Advances in pharmacology, 2016 Academic Press, 361-431.

[27] Preetha SP, Kanniappan M, Selvakumar E, Nagaraj M, Varalakshmi P. Lupeol ameliorates aflatoxin B1-induced peroxidative hepatic damage in rats. Comp Biochem Physiol C Toxicol Pharmacol, 2006, 143(3): 333-339.

[28] Qu D, Han J. Investigation of the antioxidant activity of chitooligosaccharides on mice with high-fat diet. Rev Brasil Zootecnia, 2016, 45(11): 661-666.

[29] Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited. FASEB J, 2008, 22(3): 659-661.

[30] Salvador JM, Brown-Clay JD, Fornace AJ Jr. Gadd45 in stress signaling, cell cycle control, and apoptosis. Adv Exp Med Biol, 2013, 793: 1-19.

[31] Samivel R, Subramanian U, Ali Khan A, . Lipopolysaccharide enhances genotoxicity by activating GADD45G and NF-kappaB in human corneal epithelial cells. Oxid Med Cell Longev, 2022, 2022: 4328116.

[32] Tessari EN, Kobashigawa E, Cardoso AL, Ledoux DR, Rottinghaus GE, Oliveira CA. Effects of aflatoxin B(1) and fumonisin B(1) on blood biochemical parameters in broilers. Toxins (basel), 2010, 2(4): 453-460.

[33] Wang L, Potter JJ, Rennie-Tankersley L, Novitskiy G, Sipes J, Mezey E. Effects of retinoic acid on the development of liver fibrosis produced by carbon tetrachloride in mice. Biochim Biophys Acta, 2007, 1772(1): 66-71.

[34] Wang X, He Y, Tian J, . Ferulic acid prevents aflatoxin B1-induced liver injury in rats via inhibiting cytochrome P450 enzyme, activating Nrf2/GST pathway and regulating mitochondrial pathway. Ecotoxicol Environ Saf, 2021, 224.

[35] Woo LL, Egner PA, Belanger CL, . Aflatoxin B1-DNA adduct formation and mutagenicity in livers of neonatal male and female B6C3F1 mice. Toxicol Sci, 2011, 122(1): 38-44.

[36] Wu Q, Jezkova A, Yuan Z, Pavlikova L, Dohnal V, Kuca K. Biological degradation of aflatoxins. Drug Metab Rev, 2009, 41(1): 1-7.

[37] Xia Y, Wu ZF, He R, . Simultaneous degradation of two mycotoxins enabled by a fusion enzyme in food-grade recombinant Kluyveromyces lactis. Bioresour Bioprocess, 2021, 8(1): 62.

[38] Yadav PN, Adhikari R, Marasini BP, Garai A, Adhikari HS. Synthesis and characterization of high molecular weight chitosan, and antioxidant activity of its chitosan oligosaccharide encapsulation. J Nepal Chem Soc, 2021, 42(1): 29-38.

[39] Yan JH, Chen L, Zhang L, . New insights into the persistent effects of acute exposure to AFB(1) on rat liver. Front Microbiol, 2022, 13.

[40] You JS, Zhao MY, Chen SM, . Effect of chitooligosaccharides with a specific degree of polymerization on multiple targets in T2DM mice. Bioresour Bioprocess, 2022, 9(1): 94.

[41] Zhang X, Li Q, Jiang W, . LAMA5 promotes human umbilical vein endothelial cells migration, proliferation, and angiogenesis and is decreased in preeclampsia. J Matern Fetal Neonatal Med, 2020, 33(7): 1114-1124.

[42] Zhang L, Ren R, Yang X, Ge Y, Zhang X, Yuan H. Oncogenic role of early growth response-1 in liver cancer through the regulation of the microRNA-675/sestrin 3 and the Wnt/beta-catenin signaling pathway. Bioengineered, 2021, 12(1): 5305-5322.

[43] Zou YH, Liu X, Khlentzos AM, . Liver fibrosis impairs hepatic pharmacokinetics of liver transplant drugs in the rat model. Drug Metab Pharmacokinet, 2010, 25(5): 442-449.

Funding

National Natural Science Foundation of China(31972188)

Program of Shanghai Academic Research Leader(21XD1401200)