Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Abstract

•	Chitinophaga spp. and Flavobacterium spp. were first confirmed as NDB.
•	52 NDB strains from seven genera were isolated from tobacco rhizosphere soil.
•	Strains ND6 and ND16 efficiently degraded nicotine in medium and tobacco waste compost.
•	ND16 may have a new nicotine-degrading pathway by phenotype and genome analysis.
•	Abundance of five nicotine-degrading genes in soil correlated well with nicotine concentrations.

Keywords

Nicotine-degrading bacteria (NDB) / Isolation / Metabolic pathway / Metagenomics / Compost of tobacco waste

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Funding

Hubei Tobacco Company(20191307C04)