Protective effects of Lactobacillus reuteri SJ-47 strain exopolysaccharides on human skin fibroblasts damaged by UVA radiation

Abstract

Ultraviolet rays in sunlight can cause skin damage and premature aging. This study demonstrates that Lactobacillus reuteri SJ-47 strain exopolysaccharides (EPS) protect human skin fibroblasts (HSF) under UVA radiation. During the course of the experiments, we investigate the oxidative stress protection and antiaging effects of exopolysaccharides on HSF at the biochemical, cellular, and molecular levels. The results show that EPS can increase the antioxidant capacity of cells, decrease the amount of reactive-oxygen species (ROS) and malondialdehyde (MDA), while improve the expression of antioxidant enzymes. At the same time, EPS can increase collagen content, which can effectively regulate the expression of genes in the senescence and apoptosis pathways, and delay skin photoaging caused by UVA irradiation.

Keywords

Lactobacillus reuteri SJ-47 strain exopolysaccharides / Skin photoaging / Oxidative stress

References

[1] Butler É, Lundqvist C, Axelsson J. Lactobacillusreuteri DSM 17938 as a novel topical cosmetic ingredient: a proof of concept clinical study in adults with atopic dermatitis. Microorganisms, 2020

[2] Chen F, . 6-shogaol, a active constiuents of ginger prevents UVB radiation mediated inflammation and oxidative stress through modulating NrF2 signaling in human epidermal keratinocytes (HaCaT cells). J Photochem Photobiol B, 2019, 197.

[3] DuBois M, . Colorimetric method for determination of sugars and related substances. Anal Chem, 2002, 28(3): 350-356.

[4] Florence D-C, . UV, stress and aging. Dermato-Endocrinology, 2012, 4(3): 236-240.

[5] Hassan OS, . The antioxidant, anti-apoptotic, and proliferative potency of argan oil against betamethasone-induced oxidative renal damage in rats. Biology, 2020

[6] Hou Y-Q, . Juglanthraquinone C induces intracellular ROS increase and apoptosis by activating the Akt/Foxo signal pathway in HCC cells. Oxid Med Cell Longev, 2016, 2016: 4941623.

[7] İspirli H, . Physicochemical characterisation of an α-glucan from Lactobacillusreuteri E81 as a potential exopolysaccharide suitable for food applications. Process Biochem, 2019, 79: 91-96.

[8] Jenkins G. Molecular mechanisms of skin ageing. Mech Ageing Dev, 2002, 123(7): 801-810.

[9] Jesel L, . Atrial fibrillation progression is associated with cell senescence burden as determined by p53 and p16 expression. J Clin Med, 2019, 9(1): 36-36.

[10] Jolly J, . Biosynthesis of 1,3-propanediol from glycerol with Lactobacillusreuteri : effect of operating variables. J Biosci Bioeng, 2014, 118(2): 188-194.

[11] Jose GM, Muraleedhara Kurup G. Sulfated polysaccharides from Padinatetrastromatica arrest cell cycle, prevent metastasis and downregulate angiogenic mediators in HeLa cells. Bioact Carbohydr Diet Fibre, 2017, 12: 7-13.

[12] Kim J-A, . Chitooligomers inhibit UV-A-induced photoaging of skin by regulating TGF-β/Smad signaling cascade. Carbohyd Polym, 2012, 88(2): 490-495.

[13] Kšonžeková P, . Exopolysaccharides of Lactobacillusreuteri: their influence on adherence of E. coli to epithelial cells and inflammatory response. Carbohyd Polym, 2016, 141: 10-19.

[14] Lulu C, . 1,25-Dihydroxyvitamin D exerts an antiaging role by activation of Nrf2-antioxidant signaling and inactivation of p16/p53-senescence signaling. Aging Cell, 2019, 18(3

[15] Polefka Thomas G, . Effects of solar radiation on the skin. J Cosmet Dermatol, 2012, 11(2): 134-143.

[16] Qianru S, . Protective effects of LPL-EPS-02 on human dermal fibroblasts damaged by UVA radiation. J Func Foods, 2021

[17] Qinghui Mu, Tavella Vincent J, Luo XM. Role of Lactobacillusreuteri in human health and diseases. Front Microbiol, 2018, 9: 757.

[18] Shanmugam T, Devaraj B, Ranjithkumar R. Extracellular green synthesis of chitosan-silver nanoparticles using Lactobacillusreuteri for antibacterial applications. Biocatal Agric Biotechnol, 2020, 30: 101838.

[19] Wolfgang H, . Activation of phosphatidylinositol-3'-kinase/AKT signaling is essential in hepatoblastoma survival. Clin Cancer Res: Offic J American Association for Cancer Rese, 2009, 15(14): 4538-4545.

[20] XiaoHu L, . Ginsenoside Rg1 attenuates premature ovarian failure of D-gal induced POF mice through downregulating p16INK4a and upregulating SIRT1 expression. Endocr Metab Immune Disord Drug Targets, 2021

[21] Yanpei G, . Biomarkers, oxidative stress and autophagy in skin aging. Ageing Res Rev, 2020, 59: 101036.

[22] You-Cheng H, . Zerumbone exhibits antiphotoaging and dermatoprotective properties in Ultraviolet A-irradiated human skin fibroblast cells via the activation of Nrf2/ARE defensive pathway. Oxid Med Cell Longev, 2019, 2019: 4098674.

[23] Zhixue W, Jifeng F, Jizhou X. Correlation of the expression of inflammatory factors with expression of apoptosis-related genes Bax and Bcl-2, in burned rats. Exp Ther Med, 2019, 17(3): 1790-1796.