Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source

Qiyuan Lu; Xiaoyu Shan; Weizhu Zeng; Jingwen Zhou

doi:10.1186/s40643-022-00593-5

Bioresources and Bioprocessing ›› 2022, Vol. 9 ›› Issue (1) : 109 DOI: 10.1186/s40643-022-00593-5

Research

Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source

Qiyuan Lu ¹^,²^,³^,⁴
, Xiaoyu Shan ¹^,²^,³^,⁴
, Weizhu Zeng ¹^,²^,³^,^c
, Jingwen Zhou ¹^,²^,³^,⁴^,^d

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Abstract

Pyruvic acid is an important organic acid and a key industrial raw material. It is widely used in the chemical, agricultural, and food fields. Candida glabrata is the preferred strain for pyruvic acid production. The waste yeast cell for pyruvic acid fermentation with C. glabrata are rich in protein, amino acid, nucleic acid, and vitamins, as potential and cost-effective nitrogen source raw material. In this study, the potential of C. glabrata to produce pyruvic acid using spent yeast cell dry powder was evaluated. When 30 g/L of spray-dried spent yeast cell powder was used as the seed nitrogen source, a high titer of pyruvic acid was obtained. The pyruvic acid production reached 63.4 g/L with a yield of 0.59 g/g in a 5 L bioreactor. After scale-up to a 50 L bioreactor using the fermented spent yeast cell dry powder as a seed nitrogen source, 65.1 g/L of pyruvic acid was harvested, with a yield of 0.61 g/g. This study proposes a promising

approach for increasing the pyruvic acid titer and reducing the costs.

Keywords

Pyruvic acid / Candida glabrata / Spray drying / Spent yeast cell dry powder / Fermentation optimization

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Qiyuan Lu, Xiaoyu Shan, Weizhu Zeng, Jingwen Zhou. Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source. Bioresources and Bioprocessing, 2022, 9(1): 109 DOI:10.1186/s40643-022-00593-5

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