A comprehensive review on oleaginous bacteria: an alternative source for biodiesel production

Abstract

• Biodiesel from oleaginous bacteria and its importance are summarized.

• Biochemical pathways for fatty acid synthesis are described.

• Critical biotechnological approaches for bacterial strain improvement have been discussed.

• Sustainable biodiesel production, challenges, and future possibilities have been discussed.

Keywords

Biodiesel / Bio-harvesting / Feedstock / Oleaginous microbes / Transesterification

References

[1] Abdullah MA, Nazir MS, Raza MR, . Autoclave and ultra-sonication treatments of oil palm empty fruit bunch fibers for cellulose extraction and its polypropylene composite properties. J Clean Prod, 2016, 126: 686-697.

[2] Acharya RN, Perez-Pena R. Role of comparative advantage in biofuel policy adoption in Latin America. Sustainability, 2020, 12: 1411.

[3] Adegboye MF, Ojuederie OB, Talia PM, . Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges. Biotechnol Biofuels, 2021, 14: 5.

[4] Adhikaria S, Illukpitiya P. Small-scale biodiesel production for on-farm energy security: a sustainable income diversification opportunity for oilseed producers. Biofuels, 2021

[5] Alalwana HA, Alminshidb AH, Aljaafari HAS. Promising evolution of biofuel generations. Renew Energy Focus, 2019, 8: 127-139.

[6] Ali CH, Qureshi AS, Mbadinga SM, . Biodiesel production from waste cooking oil using onsite produced purified lipase from Pseudomonas aeruginosa FWSH-1: Central composite design approach. Renew Energy, 2017, 109: 93-100.

[7] Allen J, Unlu J, Demire Y, . Integration of biology, ecology and engineering for sustainable algal-based biofuel and bioproduct biorefinery. Bioresour Bioprocess, 2018, 5: 47.

[8] Araujo K, Mahajan D, Kerr R, . Global biofuels at the crossroads: an overview of technical, policy, and investment complexities in the sustainability of biofuel development. Agriculture, 2017, 7: 32.

[9] Aron NSM, Khoo KS, Chew KW, . The Hong Phong Nguyen sustainability of the four generations of biofuels—a review. Int J Energy Resources, 2020

[10] Arora N, Patel A, Pruthi PA, Pruthi V. Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella minutissima for biodiesel production. Bioresour Technol, 2016, 213: 79-87.

[11] Arous F, Jaouani A, Mechichi T. Oleaginous microorganisms for simultaneous biodiesel production and wastewater treatment. Microbial Wastewater Treatment, 2019

[12] Babu MV, Murthy KM, Rao GAP. Production process optimization of SterculiaFoetida Methyl Esters (Biodiesel) using response surface methodology. Int J Ambient Energy, 2020

[13] Banerjee A, Banerjee C, Negi S, . Improvements in algal lipid production: a systems biology and gene editing approach. Crit Rev Biotechnol, 2017, 38: 369-385.

[14] Bao T, Zhao J, Li J, . n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases. Biores Technol, 2018, 285.

[15] Behera AR, Dutta K, Verma P, . High lipid accumulating bacteria isolated from dairy effluent scum grown on dairy wastewater as potential biodiesel feedstock. J Environ Manage, 2019, 15.

[16] Bharti RK, Srivastava S, Thakur IS. Extraction of extracellular lipids from chemoautotrophic bacteria Serratia sp. ISTD04 for production of biodiesel. Bioresour Technol, 2014, 165: 201-204.

[17] Bharti RK, Srivastava S, Thakur IS. Production and characterization of biodiesel from carbon dioxide concentrating chemolithotrophic bacteria, Serratia sp. ISTD04. Bioresour Technol, 2014, 153: 189-197.

[18] Campbell JW, Cronan JE. Bacterial fatty acid biosynthesis: targets for antibacterial drug discovery. Ann Rev Microbiol, 2010, 55: 305-332.

[19] Carsanba E, Papanikolaou S, Erten H. Production of oils and fats by oleaginous microorganisms with an emphasis given to the potential of the nonconventional yeast Yarrowialipolytica. Crit Rev Biotechnol, 2018, 38: 1230-1243.

[20] Cea M, Sangaletti-Gerhard N, Acuna P, . Screening transesterifiable lipid accumulating bacteria from sewage sludge for biodiesel production. Biotechnol Report, 2015, 8: 116-123.

[21] Cheah WY, Sankaran R, Show PL, . Pretreatment methods for lignocellulosic biofuels production: current advances, challenges and future prospects. Biofuel Res J, 2020, 25: 1115-1127.

[22] Chen J, Li J, Zhang X. Ultra-sonication application in biodiesel production from heterotrophic oleaginous microorganisms. Crit Rev Biotechnol, 2018, 38: 902-917.

[23] Cho HU, Kim YM, Choi Y, . Effects of pH control and concentration on microbial oil production from Chlorella Vulgaris cultivated in the effluent of a low-cost organic waste fermentation system producing volatile fatty acids. Bioresour Technol, 2015, 184: 245-250.

[24] Cho S, Shin J, Cho BK. Applications of CRISPR/Cas system to bacterial metabolic engineering. Int J Mol Sci, 2018, 19: 1089.

[25] Chong JWR, Yew GY, Khoo KS, . Recent advances on food waste pretreatment technology via microalgae for source of polyhydroxyalkanoates. J Environ Manag, 2021, 293.

[26] Dahman Y, Dignan C, Fiayaz A, . An introduction to biofuels, foods, livestock, and the environment. Biomass Biopolymer Based Mater Bioenergy, 2019

[27] Demirbas A. Biofuels sources, biofuel policy, biofuel economy and global biofuel projections. Energy Convers Manage, 2008, 49: 2106-2116.

[28] Enamala MK, Enamala S, Chavali M, . Production of biofuels from microalgae-a review on cultivation, harvesting, lipid extraction, and numerous applications of microalgae. Renew Sust Energ Rev, 2018, 94: 49-68.

[29] Ferrero GO, Sanchez Faba EM, Rickert AA, . Alternatives to rethink tomorrow: biodiesel production from residual and non–edible oils using biocatalyst technology. Renew Energy, 2020, 150: 128-135.

[30] Gautam R, Ansari NA, Thakur P, . Status of Bio fuel in India with production and performance characteristics: a review. Int J Ambient Energy, 2019

[31] Goswami L, Namboodiri MMT, Kumar RV, . Biodiesel production potential of oleaginous Rhodococcusopacus grown on biomass gasification wastewater. Renew Energ, 2017, 105: 400-406.

[32] Gupta N, Manikandan N, Pakshirajan K. Real–time lipid production and dairy wastewater treatment using Rhodococcusopacus in a bioreactor under fed–batch, continuous and continuous cell recycling modes for potential biodiesel application. Biofuels, 2017, 9: 1-8.

[33] Gupta J, Agarwal M, Dalai AK. An overview on the recent advancements of sustainable heterogeneous catalysts and prominent continuous reactor for biodiesel production. J Ind Eng Chem, 2020, 88: 58-77.

[34] Han F, Pei H, Hu W, . The feasibility of ultrasonic stimulation on microalgae for efficient lipid accumulation at the end of the logarithmic phase. Algal Res, 2016, 16: 189-194.

[35] Ho WWS, Ng HK, Gan S. Advances in ultrasound assisted transesterification for biodiesel production. Appl Ther Eng, 2016, 100: 553-563.

[36] Hoang PTN, Ko JK, Gong G, . Genomic and phenotypic characterization of a refactored xylose-utilizing Saccharomyces cerevisiae strain for lignocellulosic biofuel production. Biotechnol Biofuels, 2018, 11: 268.

[37] Hossain N, Mahlia TMI. Progress in physicochemical parameters of microalgae cultivation for biofuel production. Crit Rev Biotechnol, 2020, 39: 835-859.

[38] India Biofuels Annual Biofuels Annual 2019, USDA Foreign Agricultural Service, 2019.

[39] Jamal SIH, Park HS. Monoalkyl esters (biodiesel) production from wastewater sludge by esterification. Biofuels, 2019

[40] Janben HJ, Steinbuchel A. Fatty acid synthesis in Escherichia coli and its applications toward the production of fatty acid–based biofuels. Biotechnol Biofuels, 2014, 7: 1-26.

[41] Javidpour P, Pereira JH, Goh EB, . Biochemical and structural studies of NADH–dependent FabG used to increase the bacterial production of fatty acids under anaerobic conditions. Appl Environ Microbiol, 2014, 80: 497-505.

[42] John C, Paul H, Jim D (2016) International energy outlook. 2016 with projections to 2040. United States: N. p. 2016. Web. Doi:https://doi.org/10.2172/1296780

[43] Karn SK, Kumar A. Sludge: next paradigm for enzyme extraction and energy generation. Prep Biochem Biotechnol, 2019, 49: 105-116.

[44] Kaya C, Kokkulunk G. Biodiesel as alternative additive fuel for diesel engines: an experimental and theoretical investigation on emissions and performance characteristics. Energy Sour Part A Recovery Util Environ Effects, 2020

[45] Khan I, Ganesan R, Dutta JR. Probiotic lipase derived from Lactobacillusplantarum and Lactobacillusbrevis for biodiesel production from waste cooking olive oil: an alternative feedstock. I J Green Energy, 2019, 17: 62-70.

[46] Khoo KS, Chew KW, Yew GY, . Recent advances in downstream processing of microalgae lipid recovery for biofuel production. Biores Technol, 2020

[47] Kosa M, Ragauskas AJ. Lignin to lipid bioconversion by heterotrophic oleaginous Rhodococci. Renew Chem, 2013, 15: 2070-2161.

[48] Kot AM, Blazejak S, Kurcz A, . Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorulaglutinis. Elect J Biotechnol, 2017, 27: 25-31.

[49] Kumar M, Thakur IS. Municipal secondary sludge as carbon source for production and characterization of biodiesel from oleaginous bacteria. Bioresour Technol Reports, 2018, 4: 106-113.

[50] Kumar S, Gupta N, Pakshirajan K. Simultaneous lipid production and dairy wastewater treatment using Rhodococcusopacus in a batch bioreactor for potential biodiesel application. J Environ Chem Eng, 2015, 3: 1630-1636.

[51] Kumar M, Gupta J, Thakur IS. Production and optimization of polyhydroxyalkanoate from oleaginous bacteria Bacillus sp. ISTC1. Res Rev J Microbiol Biotechnol, 2016, 5: 80-89.

[52] Kumar D, Singha B, Korstad J. Utilization of lignocellulosic biomass by oleaginous yeast and bacteria for production of biodiesel and renewable diesel. Ren Sustain Energy Rev, 2017, 73: 654-671.

[53] Kumar M, Morya R, Gnansounou E, . Characterization of carbon dioxide concentrating chemolithotrophic bacterium Serratia sp. ISTD04 for production of biodiesel. Bioresour Technol, 2017, 243: 893-897.

[54] Kumar M, Rathour R, Gupta J, . Bacterial production of fatty acid and biodiesel: opportunity and challenges. Ref Biomass Resid Sust Energ Bioprod, 2020

[55] Li Z, Ding S, Chen C. Recyclable Li/NaY zeolite as a heterogeneous alkaline catalyst for biodiesel production: process optimization and kinetics study. Energ Conver Manag, 2019, 192: 335-345.

[56] Liang MH, Jiang JG. Advancing oleaginous microorganisms to produce lipid via metabolic engineering technology. Prog Lipid Res, 2013, 52: 395-408.

[57] Lotero E, Liu Y, Lopez DE, . Synthesis of biodiesel via acid catalysis. Anal Chem, 2005, 44: 5353-5363.

[58] Maa X, Gaoa Z, Wang Q, . Biodiesels from microbial oils: opportunity and challenges. Bioresource Technol, 2018, 263: 631-641.

[59] Maheshwari N, Kumar M, Thakur IS. Carbon dioxide biofixation by free air CO₂ enriched (FACE) bacterium for biodiesel production. J CO2 Utilizat, 2018, 27: 423-432.

[60] Mahlia TMI, Syazmi ZAHS, Mofijur M, . Patent landscape review on biodiesel production: technology updates. Renew Sust Energ Rev, 2020, 118.

[61] Mardhiah HH, Ong HC, Masjuki HH, . Investigation of carbon-based solid acid catalyst from Jatropha curcas biomass in biodiesel production. Energy Convers Manage, 2017, 144: 10-17.

[62] Mat Aron NS, Khoo KS, Chew KW, Ak V, . Microalgae cultivation in wastewater and potential processing strategies using solvent and membrane separation technologies. J Water Process Eng, 2021

[63] Mishra VK, Goswami R. A review of production, properties and advantages of biodiesel. Biofuels, 2017, 9: 273-289.

[64] National policy on biofuel, Government of India Ministry of New & Renewable Energy, Block No. 14, C.G.O. ComplexLodhi RoadNew Delhi–110003, https://mnre.gov.in/file–manager/UserFiles/biofuelpolicy.pdf, Retrive date 06/06/2019, 15.38 hrs.

[65] Nayan S, Kiran KD, Mark AS. Effects of nitrogen and phosphorus limitation on lipid accumulation by Chlorella kessleri str. UTEX 263 grown in darkness. J Appl Phycol, 2020, 32: 2795-2805.

[66] Nie L, Xu K, Zhong B, . Enhanced l-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum. Bioresour Bioprocess, 2022, 9: 11.

[67] Pant D, Misra S, Nizami AS, . Towards the development of a biobased economy in Europe and India. Crit Rev Biotechnol, 2019, 39: 779-799.

[68] Patel A, Arora N, Antonopoulou I. Single cell oil and ethanol production by the oleaginous yeast Trichosporonfermentans utilizing dried sweet sorghum stalks. Renew Energ, 2020, 146: 1609-1617.

[69] Patel A, Karageorgou D, Rova E, . An overview of potential oleaginous microorganisms and their role in biodiesel and omega-3 fatty acid-based industries microorganisms. Microorganisms, 2020, 8: 434.

[70] Patel A, Matsakas L, Sartaj K. Isloor AM, Asiri AM. Extraction of lipids from algae using supercritical carbon dioxide. Green sustainable process for chemical and environmental engineering and science. Supercritical Carbon Dioxide as Green Solvent, 2020, Amsterdam: Elsevier Inc, 17-39. ISBN 9780128173886

[71] Pfleger BF, Gossing M, Nielsen J. Metabolic engineering strategies for microbial synthesis of oleochemicals. Metabol Eng, 2015, 29: 1-11.

[72] Qadeer S, Khalid A, Mahmood S, . Utilization oleaginous bacteria and fungi for cleaner energy production. J Clean Product, 2017, 168: 917-928.

[73] Qi W, Lu H, Zhang Y, . Oil crop genetic modification for producing added value lipids. Crit Rev Biotechnol, 2020, 40: 777-786.

[74] Ratledge C. Fatty acid biosynthesis in microorganisms being used for Single cell oil production. Biochimie, 2004, 86: 807-815.

[75] Reddy YS, Reddy CO, Subhadra M, . Long – term storage effect on molecular interactions of biodiesels and blends. Energy Sources Part A Recovery Util Environ Effects, 2020

[76] Rottig A, Zurek PJ, Steinbuchel A. Assessment of bacterial acyltransferases for an efficient lipid production in metabolically engineered strains of E. coli. Metabol Eng, 2015, 32: 195-206.

[77] Sankaran R, Andres Parra Cruz R, Pakalapati H, . Recent advances in the pretreatment of microalgal and lignocellulosic biomass: a comprehensive review. Biores Technol, 2019

[78] Shadidi B, Alizade HHA, Najafi G. Performance and exergy analysis of a diesel engine run on petrodiesel and biodiesel blends containing mixed CeO₂ and MoO₃ nanocatalyst. Biofuels, 2020

[79] Sharholy M, Ahmad K, Vaishya R, . Municipal solid waste characteristics and management in Allahabad, India. Waste Manage, 2007, 27: 490-496.

[80] Sheth A, Sarkar D, Mukhopadhyay I. Social benefit cost and life cycle cost analysis of sustainable biodiesel bus transport in India. Int J Sustain Eng, 2020

[81] Singh D, Sharma D, Soni SL, . A review on feedstocks, production processes, and yield for different generations of biodiesel. Fuel, 2020, 262.

[82] Sinha SK, Subramanian KA, Singh HM, . Progressive trends in Bio-fuel policies in India: targets and implementation strategy. Biofuels, 2019, 10: 155-166.

[83] Sitepu IR, Garay LA, Sestric R, . Oleaginous yeasts for biodiesel: current and future trends in biology and production. Biotechnol Advan, 2014, 32: 1336-1360.

[84] Sivaramakrishnan R, Incharoensakdi A. Direct transesterification of Botryococcus sp. catalysed by immobilized lipase: ultrasound treatment can reduce reaction time with high yield of methyl ester. Fuel, 2017, 191: 363-370.

[85] Srinivasan GR, Shankar V, Sekharan SC. Influence of fatty acid composition on process optimization and characteristics assessment of biodiesel produced from waste animal fat. Energy Sources Part A Recovery Util Environ Effects, 2020

[86] Sun J, Zhang L, Loh KC. Review and perspectives of enhanced volatile fatty acids production from acidogenic fermentation of lignocellulosic biomass wastes. Bioresour Bioprocess, 2021, 8: 68.

[87] Vahid BR, Haghighi M. Urea-nitrate combustion synthesis of MgO/MgAl₂O₄ nanocatalyst used in biodiesel production from sunflower oil: influence of fuel ratio on catalytic properties and performance. Energ Conver Manag, 2016, 126: 362-372.

[88] Vasudevan PT, Briggs M. Biodiesel production–current state of the art and challenges. J Indust Microbiol Biotechnol, 2008, 35: 421-430.

[89] Vazquez VA, Estrada RAD, Flores MMA. Transesterification of non-edible castor oil (Ricinus communis L.) from Mexico for biodiesel production: a physicochemical characterization. Biofuels, 2020, 11: 753-762.

[90] Wahlen BD, Morgan MR, McCurdy AT. Biodiesel from microalgae, yeast, and bacteria: engine performance and exhaust. Energ Fuels, 2013, 27: 220-228.

[91] Wang Z, Gu S. State-of-the-art on the development of ultrasonic equipment and key problems of ultrasonic oil production technique for EOR in China. Renew Sustain Energ Review, 2017, 82: 2401-2407.

[92] Wang H, Peng X, Zhang H, . Microorganisms-promoted biodiesel production from biomass: A review. Energy Convers Manag, 2021, 12.

[93] Wang S, Zhu J, Dai L. A novel process on lipid extraction from microalgae for biodiesel production. Energy, 2016, 115: 963-968.

[94] Xu P, Ranganathan S, Fowler ZL. Genome–scale metabolic network modeling results in minimal interventions that cooperatively force carbon flux towards malonyl–CoA. Metabol Eng, 2011, 13: 578-587.

[95] Yang J, Shi W, Fang F. Exploring the feasibility of sewage treatment by algal–bacterial consortia. Crit Rev Biotechnol, 2020, 40: 169-179.

[96] Zeghloulia J, Guendouza A, Duchezb D. Valorization of co-products generated by argan oil extraction process: application to biodiesel production. Biofuels, 2021

[97] Zhang J, Gao H, Xue Q. Potential applications of microbial enhanced oil recovery to heavy oil. Crit Rev Biotechnol, 2020, 40: 459-474.

[98] Zhang Y, Peng J, Zhao H, . Engineering oleaginous yeast Rhodotorulatoruloidesfor overproduction of fatty acid ethyl esters. Biotechnol Biofuels, 2021, 14: 115.

[99] Zhang J, Tsai Y, Hsu CH. Biodiesel production from Nannochloropsisoculata cultured at stressful carbon dioxide concentration and light illumination. Biofuels, 2020

[100] Zhou X, Jin W, Wang Q. Enhancement of productivity of Chlorella pyrenoidosa lipids for biodiesel using co-culture with ammonia-oxidizing bacteria in municipal wastewater. Renew Energ, 2020, 151: 598-603.

[101] Ziolkowska JR. Biofuels technologies: An overview of feedstocks, processes, and technologies. Biofuel Sust Future, 2020

Funding

Regional Centre for Biotechnology, NCR Biotech Science Cluster 3rd Milestone, Faridabad-Gurugram Expressway, Faridabad – 120001(DBT/JRF/BET-18/I/2018/AL/123)