Coproduction of xylo-oligosaccharides and glucose from sugarcane bagasse in subcritical CO₂-assisted seawater system

Abstract

Abundant seawater resources can replace the shortage of freshwater resources. The co-production of xylo-oligosaccharides and glucose from sugarcane bagasse by subcritical CO₂-assisted seawater pretreatment was studied in this paper. We investigated the effects of pretreatment conditions of temperature, CO₂ pressure and reaction time on the yield of xylo-oligosaccharides in subcritical CO₂-assisted seawater systems. The maximum xylo-oligosaccharide yield of 68.23% was obtained at 165 °C/2 MPa/5 min. After further enzymatic hydrolysis of the solid residue, the highest glucose yield of 94.45% was obtained. In this system, there is a synergistic effect of mixed ions in seawater and CO₂ to depolymerize xylan into xylo-oligosaccharides with a lower degree of polymerization. At the same time, the addition of CO₂ increased the pore size and porosity of sugarcane bagasse, improved the efficiency of enzymatic hydrolysis and increased the yield of glucose. Therefore, this study provides a more environmentally friendly and sustainable process for the co-production of xylo-oligosaccharides and glucose from sugarcane bagasse, and improves the utilization of seawater resources.

Keywords

Sugarcane bagasse / Xylo-oligosaccharides / Subcritical CO₂-assisted seawater pretreatment / Glucose / Enzymatic hydrolysis

References

[1] Ajala EO, Ighalo JO, Ajala MA, Adeniyi AG, Ayanshola AM. Sugarcane bagasse: a biomass sufficiently applied for improving global energy, environment and economic sustainability. Bioresour Bioprocess, 2021, 8: 87.

[2] Chourasia VR, Pandey A, Pant KK, Henry RJ. Improving enzymatic digestibility of sugarcane bagasse from different varieties of sugarcane using deep eutectic solvent pretreatment. Bioresour Technol, 2021, 337: 125480.

[3] de Freitas C, Carmona E, Brienzo M. Xylooligosaccharides production process from lignocellulosic biomass and bioactive effects. Bioact Carbohydr Diet Fibre, 2019, 18: 100184.

[4] Ge S, Wu Y, Peng W, Xia C, Mei C, Cai L, Shi SQ, Sonne C, Lam SS, Tsang YF. High-pressure CO₂ hydrothermal pretreatment of peanut shells for enzymatic hydrolysis conversion into glucose. Chem Eng J, 2020, 385: 123949.

[5] Gong Z, Yang G, Song J, Zheng P, Liu J, Zhu W, Huang L, Chen L, Luo X, Shuai L. Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses. Bioresour Bioprocess, 2021, 8: 9.

[6] Gurgel LVA, Pimenta MTB, da Silva Curvelo AA. Enhancing liquid hot water (LHW) pretreatment of sugarcane bagasse by high pressure carbon dioxide (HP-CO₂). Ind Crops Prod, 2014, 57: 141-149.

[7] Huang K, Luo J, Cao R, Su Y, Xu Y. Enhanced xylooligosaccharides yields and enzymatic hydrolyzability of cellulose using acetic acid catalysis of poplar sawdust. J Wood Chem Technol, 2018, 38: 371-384.

[8] Ire FS, Ezebuiro V, Ogugbue CJ. Production of bioethanol by bacterial co-culture from agro-waste-impacted soil through simultaneous saccharification and co-fermentation of steam-exploded bagasse. Bioresour Bioprocess, 2016, 3: 26.

[9] Iruretagoyena D, Bikane K, Sunny N, Lu H, Kazarian SG, Chadwick D, Pini R, Shah N. Enhanced selective adsorption desulfurization on CO₂ and steam treated activated carbons: Equilibria and kinetics. Chem Eng J, 2020, 379: 122356.

[10] Jaichakan P, Nakphaichit M, Rungchang S, Weerawatanakorn M, Phongthai S, Klangpetch W. Two-stage processing for xylooligosaccharide recovery from rice by-products and evaluation of products: promotion of lactic acid-producing bacterial growth and food application in a high-pressure process. Food Res Int, 2021, 147: 110529.

[11] Jiang Z, Budarin VL, Fan J, Remón J, Li T, Hu C, Clark JH. Sodium chloride-assisted depolymerization of xylo-oligomers to xylose. ACS Sustain Chem Eng, 2018, 6: 4098-4104.

[12] Kamireddy SR, Li J, Tucker M, Degenstein J, Ji Y. Effects and mechanism of metal chloride salts on treatment and enzymatic digestibility of corn stover. Ind Eng Chem Res, 2013, 52: 1775-1782.

[13] Liao H, Li X, Lian Z, Xu Y, Zhang J. Two-step acetic acid/sodium acetate and xylanase hydrolysis for xylooligosaccharides production from corncob. Bioresour Technol, 2021, 342: 125979.

[14] Liao H, Xu Y, Zhang J. Efficient production of xylooligosaccharides and fermentable sugars from corncob by propionic acid and enzymatic hydrolysis. Bioresour Technol, 2021, 342: 125680.

[15] Lin W, Xing S, Jin Y, Lu X, Huang C, Yong Q. Insight into understanding the performance of deep eutectic solvent pretreatment on improving enzymatic digestibility of bamboo residues. Bioresour Technol, 2020, 306: 123163.

[16] Liu X, Wei W, Wu S. Subcritical CO₂-assisted autohydrolysis for the co-production of oligosaccharides and fermentable sugar from corn straw. Cellulose, 2019, 26: 7889-7903.

[17] Loow YL, Wu TY, Tan KA, Lim YS, Slow LF, Jahim JM, Mohammad AW, Teoh WH. Recent advances in the application of inorganic salt treatment for transforming lignocellulosic biomass into reducing sugars. J Agric Food Chem, 2015, 63: 8349-8363.

[18] Moura P, Barata R, Carvalheiro F, Gírio F, Loureiro-Dias MC, Esteves MP. In vitro fermentation of xylo-oligosaccharides from corn cobs autohydrolysis by Bifidobacterium and Lactobacillus strains. LWT, 2007, 40: 963-972.

[19] Ni J, Di J, Ma C, He Y. Valorisation of corncob into furfuryl alcohol and furoic acid via chemoenzymatic cascade catalysis. Bioresour Bioprocess, 2021, 8: 113.

[20] Nuntawat K, Bhagavathi SS, Sasithorn S, Narissara L, Sartjin P, Chaiyavat C. Optimization of alkaline pretreatment and enzymatic hydrolysis for the extraction of xylooligosaccharide from rice husk. AMB Expr, 2018, 8: 115.

[21] Park C, Lee J. Recent achievements in CO₂-assisted and CO₂-catalyzed biomass conversion reactions. Green Chem, 2020, 22(9): 2628-2642.

[22] Segal L, Creely JJ, Martin AE Jr, Conrad CM. Crystallinity of native cellulose using the X-ray diffractometer. Text Res J, 1959, 29: 786-794.

[23] Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D. Determination of structural carbohydrates and lignin in biomass. Lab Anal Proced, 2008, 1617: 1-16.

[24] Valladares-Diestra KK, de Souza Porto Vandenberghe L, Soccol CR, . A biorefinery approach for enzymatic complex production for the synthesis of xylooligosaccharides from sugarcane bagasse. Bioresour Technol, 2021, 333: 125174.

[25] Wu R, Liu W, Li L, Jiang C, Hou Q. Combination of hydrothermal and chemi-mechanical pretreatments to enhance enzymatic hydrolysis of poplar branches and insights on cellulase adsorption. Bioresour Technol, 2021, 342: 126024.

[26] Wu Y, Guo H, Rahman MS, Chen X, Zhang J, Liu Y, Qin W. Biological pretreatment of corn stover for enhancing enzymatic hydrolysis using Bacillus sp. P3. Bioresour Bioprocess, 2021, 8: 92.

[27] Xiao X, Wen J, Wang Y, Bian J, Li M, Peng F, Sun R. NMR and ESI-MS spectrometry characterization of autohydrolysis xylo-oligosaccharides separated by gel permeation chromatography. Carbohyd Polym, 2018, 195: 303-310.

[28] Yang Q, Ying W, Wen P, Zhu J, Xu Y, Zhang J. Delignification of poplar for xylo-oligosaccharides production using lactic acid catalysis. Bioresour Technol, 2021, 342: 125943.

[29] You Y, Zhang X, Li P, Lei F, Jiang J. Co-production of xylooligosaccharides and activated carbons from Camellia oleifera shell treated by the catalysis and activation of zinc chloride. Bioresour Technol, 2020, 306: 123131.

[30] Zhang H, Xu Y, Yu S. Co-production of functional xylooligosaccharides and fermentable sugars from corncob with effective acetic acid prehydrolysis. Bioresour Technol, 2017, 234: 343-349.

[31] Zhang W, You Y, Lei F, Li P, Jiang J. Acetyl-assisted autohydrolysis of sugarcane bagasse for the production of xylo-oligosaccharides without additional chemicals. Bioresour Technol, 2018, 265: 387-393.

[32] Zhang W, Zhang X, Lei F, Jiang J. Co-production bioethanol and xylooligosaccharides from sugarcane bagasse via autohydrolysis pretreatment. Renew Energ, 2020, 162: 2297-2305.

[33] Zhang W, Lei F, Li P, Zhang X, Jiang J. Co-catalysis of magnesium chloride and ferrous chloride for xylo-oligosaccharides and glucose production from sugarcane bagasse. Bioresour Technol, 2019, 291: 121839.

[34] Zhang X, Zhang W, Lei F, Yang S, Jiang J. Coproduction of xylooligosaccharides and fermentable sugars from sugarcane bagasse by seawater hydrothermal pretreatment. Bioresour Technol, 2020, 309: 123385.

[35] Zhang F, Lan W, Li Z, Zhang A, Tang B, Wang H, Wang X, Ren J, Liu C. Co-production of functional xylo-oligosaccharides and fermentable sugars from corn stover through fast and facile ball mill-assisted alkaline peroxide pretreatment. Bioresour Technol, 2021, 337: 125327.

[36] Zhang L, Zhang W, Zhang F, Jiang J. Xylo-oligosaccharides and lignin production from Camellia oleifera shell by malic acid hydrolysis at mild conditions. Bioresour Technol, 2021, 341: 125897.

[37] Zhu J, Zhang H, Jiao N, Xiao Y, Shi D, Xu Y. A green process for producing xylooligosaccharides from poplar: Endoxylanase assisted autohydrolysis, activated carbon separation, and spent liquor for rice growth. Ind Crops Prod, 2021, 174: 114187.

Funding

the National Key R&D Program of China(2019YFD1002404)

the Guangxi Key Laboratory of Chemistry Engineering of Forest Products(GXFK2002/2005)