Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast

Abstract

Steroidal compounds are of great interest in the pharmaceutical field, with steroidal drugs as the second largest category of medicine in the world. Advances in synthetic biology and metabolic engineering have enabled de novo biosynthesis of sterols and steroids in yeast, which is a green and safe production route for these valuable steroidal compounds. In this review, we summarize the metabolic engineering strategies developed and employed for improving the de novo biosynthesis of sterols and steroids in yeast based on the regulation mechanisms, and introduce the recent progresses in de novo synthesis of some typical sterols and steroids in yeast. The remaining challenges and future perspectives are also discussed.

Keywords

Sterols and steroids / De novo biosynthesis / Yeast / Metabolic engineering

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Funding

National Key Research and Development Program of China(2018YFA0901800)

Natural Science Foundation of Zhejiang Province(LZ20B060002)