A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics

Jun Zhang; Wen Luo; Zhiyuan Wang; Xiaoyan Chen; Pengmei Lv; Jingliang Xu

doi:10.1186/s40643-021-00429-8

Bioresources and Bioprocessing ›› 2021, Vol. 8 ›› Issue (1) : 77 DOI: 10.1186/s40643-021-00429-8

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A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics

Jun Zhang ¹^,²^,⁴
, Wen Luo ¹
, Zhiyuan Wang ¹
, Xiaoyan Chen ¹^,⁴
, Pengmei Lv ¹^,^e
, Jingliang Xu ¹^,³^,^f

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Abstract

Co-cultivation was firstly carried out for the co-production of D-psicose and lipase.

The carbon source glucose and fructose were fully utilized during co-culture.

The yield of D- psicose was 11.70 g/L, and the conversion rate reached 69.54%.

Self-induced expression of lipase was realized with enzyme activity of 16.03 U/mg.

Metabolomics analyses were used to explore the co-culture effects of mixed strains.

Keywords

D-Psicose / Lipase / Escherichia coli / Bacillus subtilis / Co-culture / Metabolomics

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Jun Zhang, Wen Luo, Zhiyuan Wang, Xiaoyan Chen, Pengmei Lv, Jingliang Xu. A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics. Bioresources and Bioprocessing, 2021, 8(1): 77 DOI:10.1186/s40643-021-00429-8

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