Effects of different sodium salts and nitrogen sources on the production of 3-hydroxybutyrate and polyhydroxybutyrate by Burkholderia cepacia

Abstract

The effects of NaCl, Na₂SO₄, Na₂HPO₄, and Na₃C₆H₅O₇ on the production of 3-hydroxybutyrate, polyhydroxybutyrate, and by-products by Burkholderia cepacia. Proper addition of Na₃C₆H₅O₇ can significantly promote the production of 3-hydroxybutyric acid and polyhydroxybutyrate. The concentration, productivity, and yield of 3-hydroxybutyrate were increased by 48.2%, 55.6%, and 48.3% at 16 mM Na₃C₆H₅O₇. The increases of 80.1%, 47.1%, and 80.0% in the concentration, productivity, and yield of polyhydroxybutyrate were observed at 12 mM Na₃C₆H₅O₇. Na₂SO₄ and Na₂HPO₄ also have positive effects on the production capacity of 3-hydroxybutyrate and polyhydroxybutyrate within a certain range of concentration. NaCl is not conducive to the improvement of fermentation efficiency. Compared with a single nitrogen source, a mixed nitrogen source is more conducive to enhancing the production of 3-hydroxybutyrate and polyhydroxybutyrate.

Keywords

3-Hydroxybutyrate / Polyhydroxybutyrate / Burkholderia cepacia / Sodium salts / Nitrogen source

References

[1] Alcântara JM, Distante F, Storti G, Moscatelli D, Morbidelli M, Sponchioni M. Current trends in the production of biodegradable bioplastics: the case of polyhydroxyalkanoates. Biotechnol Adv, 2020, 2: 107582.

[2] Anjum A, Zuber M, Zia KM, Noreen A, Anjum MN, Tabasum S. Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: a review of recent advancements. Int J Biol Macromol, 2016, 89: 161-174.

[3] Aramvash A, Moazzeni Zavareh F, Gholami Banadkuki N. Comparison of different solvents for extraction of polyhydroxybutyrate from Cupriavidusnecator. Eng Life Sci, 2018, 18(1): 20-28.

[4] Azizi N, Najafpour G, Younesi H. Acid pretreatment and enzymatic saccharification of brown seaweed for polyhydroxybutyrate (PHB) production using Cupriavidusnecator. Int J Biol Macromol, 2017, 101: 1029-1040.

[5] Bhatia SK, Shim YH, Jeon JM, Brigham CJ, Kim YH, Kim HJ, Seo HM, Lee JH, Kim JH, Yi DH, Lee YK. Starch based polyhydroxybutyrate production in engineered Escherichiacoli. Bioprocess Biosyst Eng, 2015, 38(8): 1479-1484.

[6] Blevins HM, Blue MD, Cobbs BD, Ricotilli TA, Kyler SL, Shuey CT, Thompson WD, Baron SF. Characterization of an extracellular polyhydroxyalkanoate depolymerase from streptomyces sp. SFB5A. J Bioremediat Biodegrad, 2018

[7] Bomrungnok W, Arai T, Yoshihashi T, Sudesh K, Hatta T, Kosugi A. Direct production of polyhydroxybutyrate from waste starch by newly-isolated Bacillus aryabhattai T34–N4. Environ Technol, 2020, 41(25): 3318-3328.

[8] Chen Y, Li S, Xiong J, Li Z, Bai J, Zhang L, Ye Q, Ouyang P, Ying H. The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5′-monophosphate by Saccharomycescerevisiae. Appl Microbiol Biotechnol, 2010, 86(1): 75-81.

[9] Dalsasso RR, Pavan FA, Bordignon SE, de Aragão GM, Poletto P. Polyhydroxybutyrate (PHB) production by Cupriavidusnecator from sugarcane vinasse and molasses as mixed substrate. Process Biochem, 2019, 85: 12-18.

[10] Dañez JC, Requiso PJ, Alfafara CG, Nayve FR Jr, Ventura JR. Optimization of fermentation factors for polyhydroxybutyrate (PHB) production using Bacillusmegaterium PNCM 1890 in simulated glucose–xylose hydrolysates from agricultural residues. Philipp J Sci, 2020, 149(1): 163-175.

[11] Dietrich D, Illman B, Crooks C. Differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from Burkholderiacepacia and Pseudomonaspseudoflava. BMC Res Notes, 2013, 6(1): 1-4.

[12] Doi Y, Kawaguchi Y, Nakamura Y, Kunioka M. Nuclear magnetic resonance studies of poly (3-hydroxybutyrate) and polyphosphate metabolism in Alcaligeneseutrophus. Appl Environ Microbiol, 1989, 55(11): 2932-2938.

[13] El-Nahrawy S, El-Kodoos A, Rizk Y, Belal ES, El-Shouny W. Production of poly-β-hydroxybutyrate (PHB) by Azotobacter sp. isolated from different sources. Environ Biodivers Soil Secur, 2018, 2(8): 183-192.

[14] Freches A, Lemos PC. Microbial selection strategies for polyhydroxyalkanoates production from crude glycerol: effect of OLR and cycle length. New Biotechnol, 2017, 39: 22-28.

[15] García A, Ferrer P, Albiol J, Castillo T, Segura D, Peña C. Metabolic flux analysis and the NAD (P) H/NAD (P)+ ratios in chemostat cultures of Azotobacter vinelandii. Microb Cell Fact, 2018, 17(1): 1-3.

[16] García G, Sosa-Hernández JE, Rodas-Zuluaga LI, Castillo-Zacarías C, Iqbal H, Parra-Saldívar R. Accumulation of PHA in the microalgae Scenedesmus sp. under nutrient-deficient conditions. Polymers, 2021, 13(1): 131.

[17] Jan S, Roblot C, Courtois J, Courtois B, Barbotin JN, Seguin JP. 1H NMR spectroscopic determination of poly 3-hydroxybutyrate extracted from microbial biomass. Enzyme Microb Technol, 1996, 18(3): 195-201.

[18] Kessler B, Witholt B. Factors involved in the regulatory network of polyhydroxyalkanoate metabolism. J Biotechnol, 2001, 86(2): 97-104.

[19] Kocharin K, Nielsen J. Specific growth rate and substrate dependent polyhydroxybutyrate production in Saccharomycescerevisiae. AMB Express, 2013, 3(1): 1-6.

[20] Koller M. A review on established and emerging fermentation schemes for microbial production of Polyhydroxyalkanoate (PHA) biopolyesters. Fermentation, 2018, 4(2): 30.

[21] Lai YH, Lan JC. Enhanced polyhydroxybutyrate production through incorporation of a hydrogen fuel cell and electro-fermentation system. Int J Hydrogen Energy, 2021, 46(31): 16787-16800.

[22] Leong YK, Show PL, Lan JC, Krishnamoorthy R, Chu DT, Nagarajan D, Yen HW, Chang JS. Application of thermo-separating aqueous two-phase system in extractive bioconversion of polyhydroxyalkanoates by Cupriavidusnecator H16. Biores Technol, 2019, 287: 121474.

[23] Li M, Eskridge KM, Wilkins MR. Optimization of polyhydroxybutyrate production by experimental design of combined ternary mixture (glucose, xylose and arabinose) and process variables (sugar concentration, molar C: N ratio). Bioprocess Biosyst Eng, 2019, 42(9): 1495-1506.

[24] Mohd Zain NF, Paramasivam M, Tan JS, Lim V, Lee CK. Response surface methodology optimization of polyhydroxyalkanoate production by Burkholderiacepacia BPT1213 using waste glycerol from palm oil-based biodiesel production. Biotechnol Prog, 2021, 37(1): e3077.

[25] Moreno P, Yañez C, Cardozo NS, Escalante H, Combariza MY, Guzman C. Influence of nutritional and physicochemical variables on PHB production from raw glycerol obtained from a Colombian biodiesel plant by a wild-type Bacillusmegaterium strain. New Biotechnol, 2015, 32(6): 682-689.

[26] Park SH, Kim GB, Kim HU, Park SJ, Choi JI. Enhanced production of poly-3-hydroxybutyrate (PHB) by expression of response regulator DR1558 in recombinant Escherichiacoli. Int J Biol Macromol, 2019, 131: 29-35.

[27] Pereira JR, Araújo D, Freitas P, Marques AC, Alves VD, Sevrin C, Grandfils C, Fortunato E, Reis MA, Freitas F. Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization. Int J Biol Macromol, 2021, 167: 85-92.

[28] Ramezani M, Amoozegar MA, Ventosa A. Screening and comparative assay of poly-hydroxyalkanoates produced by bacteria isolated from the Gavkhooni Wetland in Iran and evaluation of poly-β-hydroxybutyrate production by halotolerant bacterium Oceanimonas sp. GK1. Ann Microbiol, 2015, 65(1): 517-526.

[29] Ren Q, Ruth K, Thöny-Meyer L, Zinn M. Enatiomerically pure hydroxycarboxylic acids: current approaches and future perspectives. Appl Microbiol Biotechnol, 2010, 87(1): 41-52.

[30] Rodrigues PR, Nunes J, Lordelo LN, Druzian JI. Assessment of polyhydroxyalkanoate synthesis in submerged cultivation of Cupriavidusnecator and Burkholderiacepacia strains using soybean as substrate. Braz J Chem Eng, 2019, 36(1): 73-83.

[31] Rodríguez-Contreras A, Koller M, Braunegg G, Marqués-Calvo MS. Poly [(R)-3-hydroxybutyrate] production under different salinity conditions by a novel Bacillusmegaterium strain. New Biotechnol, 2016, 33(1): 73-77.

[32] Salgaonkar BB, Bragança JM. Utilization of sugarcane bagasse by Halogeometricumborinquense strain E3 for biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyvalerate). Bioengineering, 2017, 4(2): 50.

[33] Samantaray S, Mallick N. Production and characterization of poly-β-hydroxybutyrate (PHB) polymer from Aulosirafertilissima. J Appl Phycol, 2012, 24(4): 803-814.

[34] Sharma V, Misra S, Srivastava AK. Developing a green and sustainable process for enhanced PHB production by Azohydromonasaustralica. Biocatal Agric Biotechnol, 2017

[35] Sirohi R, Gaur VK, Pandey AK, Sim SJ, Kumar S. Harnessing fruit waste for poly-3-hydroxybutyrate production: a review. Biores Technol, 2021

[36] Thapa C, Shakya P, Shrestha R, Pal S, Manandhar P. Isolation of polyhydroxybutyrate (PHB) producing bacteria, optimization of culture conditions for PHB production, extraction and characterization of PHB. Nepal J Biotechnol, 2018, 6(1): 62-68.

[37] Uchino K, Saito T, Gebauer B, Jendrossek D. Isolated poly (3-hydroxybutyrate) (PHB) granules are complex bacterial organelles catalyzing formation of PHB from acetyl coenzyme A (CoA) and degradation of PHB to acetyl-CoA. J Bacteriol, 2007, 189(22): 8250-8256.

[38] Wang Y, Liu S. Production of (R)-3-hydroxybutyric acid by Burkholderiacepacia from wood extract hydrolysates. AMB Express, 2014, 4(1): 1.

[39] Wang P, Qiu YQ, Chen XT, Liang XF, Ren LH. Metabolomic insights into polyhydroxyalkanoates production by halophilic bacteria with acetic acid as carbon source. Biosci Biotechnol Biochem, 2019, 83(10): 1955-1963.

[40] Wang J, Huang J, Jiang S, Zhang J, Zhang Q, Ning Y, Fang M, Liu S. Parametric optimization and kinetic study of l-lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells. Biotechnol Appl Biochem, 2020

[41] Wei YH, Chen WC, Wu HS, Janarthanan OM. Biodegradable and biocompatible biomaterial, polyhydroxybutyrate, produced by an indigenous Vibrio sp. BM-1 isolated from marine environment. Mar Drugs, 2011, 9(4): 615-624.

[42] Wood JM. Bacterial responses to osmotic challenges. J Gen Physiol, 2015, 145(5): 381-388.

[43] Yadav J, Balabantaray S, Patra N. Statistical optimization of fermentation conditions for the improved production of poly-β-hydroxybutyrate from Bacillussubtilis. Chem Eng Commun, 2017, 204(10): 1122-1128.