Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach

Abstract

Extensive decoration of backbones is a major factor resulting in resistance of enzymatic conversion in hemicellulose and other branched polysaccharides. Employing debranching enzymes is the main strategy to overcome this kind of recalcitrance at present. A carbohydrate-binding module (CBM) is a contiguous amino acid sequence that can promote the binding of enzymes to various carbohydrates, thereby facilitating enzymatic hydrolysis. According to previous studies, CBMs can be classified into four types based on their preference in ligand type, where Type III and IV CBMs prefer to branched polysaccharides than the linear and thus are able to specifically enhance the hydrolysis of substrates containing side chains. With a role in dominating the hydrolysis of branched substrates, Type III and IV CBMs could represent a non-catalytic approach in overcoming side-chain recalcitrance.

Keywords

Carbohydrate-binding module / Protein structure / Protein-carbohydrate recognition / CBM fusion / Hemicellulose / Side-chain recalcitrance / Lignocellulose conversion

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Funding

National Natural Science Foundation of China(31970036)

The Natural Science Foundation of the Jiangsu Higher Education Institutions of China(20KJB180001)

Natural Science Foundation of Jiangsu Province(BK20171163)