Current understanding of substrate specificity and regioselectivity of LPMOs

Abstract

Renewable biomass such as cellulose and chitin are the most abundant sustainable sources of energy and materials. However, due to the low degradation efficiency of these recalcitrant substrates by conventional hydrolases, these biomass resources cannot be utilized efficiently. In 2010, the discovery of lytic polysaccharide monooxygenases (LPMOs) led to a major breakthrough. Currently, LPMOs are distributed in 7 families in CAZy database, including AA9–11 and AA13–16, with different species origins, substrate specificity and oxidative regioselectivity. Effective application of LPMOs in the biotransformation of biomass resources needs the elucidation of the molecular basis of their function. Since the discovery of LPMOs, great advances have been made in the study of their substrate specificity and regioselectivity, as well as their structural basis, which will be reviewed below.

Keywords

Lytic polysaccharide monooxygenase / LPMO / Substrate specificity / Regioselectivity

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Funding

Guangdong Province Science and Technology Innovation Strategy Special Fund(2018B020206001)

the GDAS’ Special Project of Science and Technology Development(2018GDASCX-0909)

the Science and Technology Plan Project of Guangdong Province(2016A010105013)

National Natural Science Foundation of China(31400681)