Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Abstract

Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

Keywords

Reaction system / Keratin residues / Biotechnological processes

References

[1] Abdel-Fattah AM, El-Gamal MS, Ismail SA, Emran MA, Hashem AM. Biodegradation of feather waste by keratinase produced from newly isolated Bacillus licheniformis ALW1. J Genet Eng Biotechnol, 2018, 16: 311-318.

[2] Anbu P, Gopinath SCB, Hilda A. Purification of keratinase from poultry farm isolate-Scopulariopsis brevicaulis and statistical optimization of enzyme activity. Enzyme Microb Technol, 2005, 36: 639-647.

[3] ANTON PAAR. Reator de Micro-ondas Monowave 100 ANTON PAAR. Available in: https://www.anton-paar.com/br-pt/produtos/detalhes/sintese-assistida-por-micro-ondas-monowave-400200/. Accessed 25 May 2018

[4] Azmi NA, Idris A, Yusof NSM. Ultrasonic technology for value added products from feather keratin. Ultrason Sonochem, 2018, 47: 99-107.

[5] Barone JR, Schmidt WF, Gregoire NT. Extrusion of Feather Keratin. J Appl Polym Sci, 2006, 100: 1432-1442.

[6] Barton S, Bullock C, Weir D. The effects of ultrasound on the activities of some glycosidase enzymes of industrial importance. Enzyme Microb Technol, 1996, 18: 190-194.

[7] BIOVERA. PROCESSADOR ULTRASSÔNICO/SONICADOR DE PONTEIRA. 2019. Available in: https://www.biovera.com.br/noticias/saiba-tudo-sobre-processador-ultrassonico-sonicador-de-ponteira-sonicador/. Accessed 25 May 2020

[8] Brandelli A. Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond. Food Bioproc Tech, 2008, 1: 105-116.

[9] Brandelli A, Daroit DJ, Riffel A. Biochemical features of microbial keratinases and their production and applications. Appl Environ Microbiol, 2010, 85: 1735-1750.

[10] Brandelli A, Sala L, Kalil SJ. Microbial enzymes for bioconversion of poultry waste into added-value products. Food Res Int, 2015, 73: 3-12.

[11] Bressollier P, Letourneau F, Urdaci M, Verneuil B. Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus. Appl Environ Microbiol, 1999, 65: 2570-2578.

[12] Călin M, Constantinescu-Aruxandei D, Alexandrescu E, Răut I, Doni MB, Arsene ML, Oancea F, Jecu L, Lazăr V. Degradation of keratin substrates by keratinolytic fungi. Electron J Biotechn, 2017, 28: 101-112.

[13] Choińska-Pulit A, Łaba W, Rodziewicz A. Enhancement of pig bristles waste bioconversion by inoculum of keratinolytic bacteria during composting. Waste Manage, 2019, 84: 269-276.

[14] ECO-SONICS. Desruptor de células. Available in: https://www.ecosonics.com.br/produto/20/desruptor-de-celulas. Accessed 02 June 2018

[15] Fang Z, Zhang J, Liu B, Du G, Chen J. Biodegradation of wool waste and keratinase production in scale-up fermenter with different strategies by Stenotrophomonas maltophilia BBE11-1. Bioresour Technol, 2013, 140: 286-291.

[16] Fang Z, Zhang J, Liu C, Du G, Chen J. Biochemical characterization of three keratinolytic enzymes from Stenotrophomonas maltophilia BBE11-1 for biodegrading keratin wastes. Int Biodeterior Biodegrad, 2013, 82: 166-172.

[17] Fegade SL, Tande BM, Cho H, Seames WS, Sakodynskaya I, Muggli DS, Kozliak EI. Aromatization of propylene over HZSM-5: a design of experiments (DOE) approach. Chem Eng Commun, 2013, 200(8): 1039-1056.

[18] Feng H, Barbosa-Cánovas GV, Weiss J. Ultrasound technologies for food and bioprocessing, 2011, New York: Springer.

[19] Gegeckas A, Šimkutė A, Gudiukaitė R, Čitavičius DJ. Characterization and application of keratinolytic peptidases from Bacillus spp. Int J Biol Macromol, 2018, 113: 1206-1213.

[20] Golunski S M, Scapini T, Modkovski T A, Marques C T, Camargo A F, Preczeski K P, Dalla Rosa C, Baldissarelli D P, Mulinari J, Venturin B, Vargas G D L P, Buffon J G, Mossi A J, Treichel H (2017) Commercial and Noncommercial Peroxidases Activity under Ultrasound and Microwave Treatment: a Pretreatment to Improve Wastewater Treatment. J Braz Chem Soc 28: 1890-1895. https://doi.org/10.21577/0103-5053.20170023

[21] Grintsevich EE, Adzerikho IE, Mrochek AG, Metelitza DI. Polydisulfides of substituted phenols as effective protectors of peroxidase against inactivation by ultrasonic cavitation. Biochemistry, 2001, 66: 740-746.

[22] Gupta R, Ramnani P. Microbial keratinases and their prospective applications: an overview. Appl Environ Microbiol, 2006, 70: 21-33.

[23] Hamiche S, Mechri S, Khelouia L, Annane R, El Hattab M, Badis A, Jaouadi B. Purification and biochemical characterization of two keratinases from Bacillus amyloliquefaciens S13 isolated from marine brown alga Zonaria tournefortii with potential keratin-biodegradation and hide-unhairing activities. Int J Biol Macromol, 2019, 122: 758-769.

[24] Holkar CR, Jain SS, Jadhav AJ, Pinjari DV. Valorization of keratin waste. Process Saf Environ, 2018, 115: 85-98.

[25] Ire FS, Onyenama AC. Effects of some cultural conditions on keratinase production by Bacillus licheniformis Strain NBRC 14206. J Adv Biol Biotechnol, 2017, 13: 1-13.

[26] Jaouadi NZ, Rekik H, Badis A, Trabelsi S, Belhoul M, Yahiaoui AB, Aicha HB, Toumi A, Bejar S, Jaouadi B. Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities. PLoS ONE, 2013, 8: 1-17.

[27] Jin J, Ma H, Qu W, Wang K, Zhou C, He R, Luo L, Owusu J. Effects of multi-frequency power ultrasound on the enzymolysis of corn gluten meal: kinetics and thermodynamics study. Ultrason Sonochem, 2015, 27: 46-53.

[28] Kapturowska AU, Stolarzewicz IA, Krzyczkowska J. Studies on the lipolytic activity of sonicated enzymes from Yarrowia lipolytica. Ultrason Sonochem, 2012, 19: 186-191.

[29] Kaul S, Sumbali G. Keratinolysis by poultry farm soil fungi. Mycopathologia, 1997, 139: 137-140.

[30] Khan NR, Rathod VK. Microwave assisted enzymatic synthesis of speciality esters: a mini - review. Process Biochem, 2018, 75: 89-98.

[31] Korniłłowicz-Kowalska T, Bohacz J. Biodegradation of keratin waste: theory and practical aspects. Waste Manage, 2011, 31: 1689-1701.

[32] Kothari D, Rani A, Goyal A (2017) Keratinases. In: Pandey A, Negi S, Soccol C R (ed) Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products, 1° edn. Elsevier. doi: https://doi.org/10.1016/B978-0-444-63662-1.00019-1

[33] Kuijpers MWA, Kemmere MF, Keurentjes JTF. Calorimetric study of the energy efficiency for ultrasound-induced radical formation. Ultrasonics, 2002, 40: 675-678.

[34] Kunert K. Biochemical mechanism of keratin degradation by the actinomycete Streptomyces fradiae and the fungus Microsporum gypseum: a comparison. J Basic Microbiol, 1989, 29: 597-604.

[35] Kushwaha RKS. The in vitro degradation of Peacock feathers by some fungi. Mykosen, 1983, 26: 324-326.

[36] Łaba W, Kopeć W, Chorążyk D, Kancelista A. Biodegradation of pretreated pig bristles by Bacillus cereus B5esz. Int Biodeterior Biodegrad, 2015, 100: 116-123.

[37] Leaes EX, Lima D, Miklasevicius L, Ramon AP, Dal Prá V, Bassaco MM, Terra LM, Mazutti MA. Effect of ultrasound-assisted irradiation on the activities of α-amylase and amyloglucosidase. Biocatal Agric Biotechnol, 2013, 2: 21-25.

[38] Leonelli C, Mason TJ. Microwave and ultrasonic processing: now a realistic option for industry. Chem Eng Process, 2010, 49: 885-900.

[39] Lippert T, Bandelin J, Musch A, Drewes JE, Koch K. Energy-positive sewage sludge pre-treatment with a novel ultrasonic flatbed reactor at low energy input. Bioresour Technol, 2018, 264: 298-305.

[40] Lopes LC, Barreto MTM, Gonçalves KM, Alvarez HM, Heredia MF, Souza ROMA, Cordeiro Y, Dariva C, Fricks AT. Stability and structural changes of horseradish peroxidase: microwave versus conventional heating treatment. Enzyme Microb Technol, 2015, 69: 10-18.

[41] Ma H, Huang L, Jia J, He R, Luo L, Zhu W. Effect of energy-gathered ultrasound on Alcalase. Ultrason Sonochem, 2011, 18: 419-424.

[42] Manasseh R, Tho P, Ooi A, Petrovic-Duran K, Zhu Y. Cavitation microstreaming and material transport around microbubbles. Phys Procedia, 2010, 3: 427-432.

[43] Mason TJ, Cobley AJ, Graves JE, Morgan D. New evidence for the inverse dependence of mechanical and chemical effects on the frequency of ultrasound. Ultrason Sonochem, 2011, 18: 226-230.

[44] Mazinani SA, DeLong B, Yan H. Microwave radiation accelerates trypsin-catalyzed peptide hydrolysis at constant bulk temperature. Tetrahedron Lett, 2015, 56: 5804-5807.

[45] Mazotto AM, Melo ACN, Macrae A, Rosado AS, Peixoto R, Cedrola SML, Couri S, Zingali RB, Villa ALV, Rabinovitch L, Chaves JQ, Vermelho AB. Biodegradation of feather waste by extracellular keratinases and gelatinases from Bacillus spp. World J Microbiol Biotechnol, 2011, 27: 1355-1365.

[46] Mazotto AM, Couri S, Damaso MCT, Vermelho AB. Degradation of feather waste by Aspergillus niger keratinases: comparison of submerged and solid-state fermentation. Int Biodeterior Biodegrad, 2013, 85: 189-195.

[47] McClements DJ. Advances in the application of ultrasound in food analysis and processing. Trends Food Sci Tech, 1995, 6: 293-299.

[48] Mulinari J, Venturin B, Sbardelotto M, Dall Agnol A, Scapini T, Camargo AF, Baldissarelli DP, Modkovski TA, Rossetto V, Dalla Rosa C, Reichert FW Jr, Golunski SM, Vargas GDLP, Dalla Rosa C, Mossi AJ, Treichel H. Ultrasound-assisted hydrolysis of waste cooking oil catalyzed by homemade lipases. Ultrason Sonochem, 2017, 35: 313-318.

[49] Okoroma EA, Garelick H, Abiola OO, Purchase D. Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather. Int Biodeterior Biodegrad, 2012, 74: 54-60.

[50] Onifade AA, Al-Sane NA, Al-Musallam AA, Al-Zarban S. A Review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour Technol, 1998, 66: 1-11.

[51] Ovsianko SL, Chernyavsky EA, Minchenya VT, Adzerikho IE, Shkumatov VM. Effect of ultrasound on activation of serine proteases precursors. Ultrason Sonochem, 2005, 12: 219-223.

[52] Paul T, Das A, Mandal A, Halder SK, Jana A, Maity C, DasMohapatra PK, Pati BR, Mondal KC. An efficient cloth cleaning property of a crude keratinase combined with detergent: towards industrial viewpoint. J Clean Prod, 2014, 66: 672-684.

[53] Potapovich MV, Eremin AN, Metelitza DI. Kinetics of catalase inactivation induced by ultrasonic cavitation. Appl Biochem Micro, 2003, 39: 140-146.

[54] Preczeski K P, Dalastra C, Czapela F F, Kubeneck S, Scapini T, Camargo A F, Zanivan J, Bonatto C, Stefanski F S, Venturin B, Fongaro G, Treichel H (2020) Fusarium oxysporum and Aspergillus sp. as keratinase producers using swine hair from agroindustrial residues. Fronti Bioeng Biotech. https://doi.org/10.3389/fbioe.2020.00071

[55] Preczeski KP, Kamanski AB, Scapini T, Camargo AF, Modkoski TA, Rossetto V, Venturin B, Mulinari J, Golunski SM, Mossi AJ, Treichel H. Efficient and low-cost alternative of lipase concentrating aiming at the application in the treatment of waste cooking oils. Bioprocess Biosyst Eng, 2018, 41: 851-857.

[56] Resa P, Elvira L, Sierra C, Espinosa FM. Ultrasonic velocity assay of extracellular invertase in living yeasts. Anal Biochem, 2009, 384: 68-73.

[57] Riffel A, Brandelli A. Keratinolytic bacteria isolated from feather waste. Braz J Microbiol, 2006, 37: 395-399.

[58] Rodrigues M I, Iemma A F (2014) Protimiza Experimental Design. Available in: http://experimental-design.protimiza.com.br/. Accessed 10 January 2019

[59] Santos RMDB, Firmino AAP, Sá CM, Felix CR. Keratinolytic activity of Aspergillus fumigatus Fresenius. Curr Microbiol, 1996, 33: 364-370.

[60] Scott JA, Untereiner WA. Determination of keratin degradation by fungi using keratin azure. Med Mycol J, 2004, 42: 239-246.

[61] SIGMA-ALDRICH. K4519 SIGMA Keratinase. Available in: https://www.sigmaaldrich.com/catalog/product/sigma/k4519?lang=pt&region=BR>. Accessed 28 May 2018

[62] Su C, Gong J, Zhang R, Tao L, Dou W, Zhang D, Li H, Lu Z, Xu Z, Shi J. A novel alkaline surfactant-stable keratinase with superior feather-degrading potential based on library screening strategy. Int J Biol Macromol, 2017, 95: 404-411.

[63] Thankaswamy SR, Sundaramoorthy S, Palanivel S, Ramudu KN. Improved microbial degradation of animal hair waste from leather industry using Brevibacterium luteolum (MTCC 5982). J Clean Prod, 2018, 189: 701-708.

[64] Wang LC. Soybean protein agglomeration: promotion by ultrasonic treatment. J Agr Food Chem, 1981, 29: 177-180.

[65] Wang J, Yanping C, Sun B, Wang C, Mo Y. Effect of ultrasound on the activity of alliinase from fresh garlic. Ultrason Sonochem, 2011, 18: 534-540.

[66] Wang F, Chen Z, Zhu H. An efficient enzymatic modification of lily polysaccharide in ionic liquid under ultrasonic irradiation. Biochem Eng J, 2013, 79: 25-28.

[67] Yadav GD, Borkar IV. Kinetic and mechanistic investigation of microwave-assisted lipase catalyzed synthesis of citronellyl acetate. Ind Eng Chem Res, 2009, 48: 7915-7922.

[68] Yang C, Fang TJ. Kinetics for enzymatic hydrolysis of rice hulls by the ultrasonic pretreatment with a bio-based basic ionic liquid. Biochem Eng J, 2015, 100: 23-29.

[69] Yusof NSM, Ashokkumar M. Sonochemical synthesis of gold nanoparticles by using high intensity focused ultrasound. ChemPhysChem, 2015, 16: 775-781.

[70] Yusuf I, Ahmad SA, Phang LY, Syed MA, Shamaan NA, Khalil KA, Dahalan FA, Shukor MY. Keratinase production and biodegradation of polluted secondary chicken feather wastes by a newly isolated multi heavy metal tolerant bacterium-Alcaligenes sp. AQ05-001. J Environ Manage, 2016, 183: 182-195.

[71] Zou S, Wang X, Chen Y, Wan H, Feng Y. Enhancement of biogas production in anaerobic co-digestion by ultrasonic pretreatment. Energ Convers Manage, 2016, 112: 226-235.

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