Seafood waste: a source for preparation of commercially employable chitin/chitosan materials

Abstract

Modern seafood processing practices result in amassment of a large volume of waste products, i.e., skin, head, tails, shells, scales, backbones, etc. These waste products may often encompass several high-value products which are still untapped due to the dearth of appropriate management. Moreover, inadequate disposal of waste also has negative implications on both environment and human health. This seafood waste often contains a huge amount of chitin, a polysaccharide that exhibits exceptional inherent characteristics including biocompatibility, biodegradability, antimicrobial, antitumor and antioxidant activities. The present review summarizes the existing methods for recovery of chitin and its derivatives from marine waste. The preparation of chitin nanoparticles was discussed along with blending of chitin and chitosan with other biopolymers. The recent trends of the application of chitin and chitosan nanostructures in various sectors were explored. This review is an attempt to highlight the extraction methods of chitin and chitosan from marine waste resources and its transformation into valuable commercial products as a solution to waste management.

Keywords

Chitin / Chitosan / N-Acetyl glucosamine / Biopolymers / Composites / Scaffolds

References

[1] Aam BB, Heggset EB, Norberg AL, Sorlie M, Varum KM, Eijsink VG. Production of chitooligosaccharides and their potential applications in medicine. Mar Drugs, 2010, 8: 1482-1517.

[2] Abdelmalek BE, Sila A, Haddar A, Bougatef A, Ayadi MA. β-Chitin and chitosan from Squid gladius: biological activities of chitosan and its application as clarifying agent for apple juice. Int J Biol Macromol, 2017, 104: 953-962.

[3] Abdou ES, Nagy KSA, Elsabee MZ. Extraction and characterization of chitin and chitosan from local sources. Bioresour Technol, 2008, 99: 1359-1367.

[4] Abdulkarim A, Isa MT, Abdulsalam S, Muhammad AJ, Ameh AO. Extraction and characterisation of chitin and chitosan from Mussel Shell. Extraction, 2013, 3(2): 108-114.

[5] Abul A, Samad S, Huq D, Moniruzzaman M, Masum M. Textile dye removal from wastewater effluents using Chitosan–Zno nanocomposite. J Textile Sci Eng, 2015, 5(200): 2.

[6] Agarwal S, Greiner A, Wendorff JH. Functional materials by electrospinning of polymers. Prog Polym Sci, 2013, 38: 963-991.

[7] Ahamed MIN, Sankar S, Kashif PM, Basha SKH, Sastry TP. Evaluation of biomaterial containing regenerated cellulose and chitosan incorporated with silver nanoparticles. Int J Biol Macromol, 2015, 72: 680-686.

[8] Ahmad M, Ahmed S, Swami BL, Ikram S. Adsorption of heavy metal ions: role of chitosan and cellulose for water treatment. Langmuir, 2015, 79: 109-155.

[9] Aiba SI. Studies on chitosan: 3. evidence for the presence of random and block copolymer structures in partially N-acetylated chitosans. Int J Biol Macromol, 1991, 13: 40-44.

[10] Ameeduzzafar SS, Imam SN, Bukhari JA, Ahmad AA. Formulation and optimization of levofloxacin loaded chitosan nanoparticle for ocular delivery: in-vitro characterization, ocular tolerance and antibacterial activity. Int J Biol Macromol, 2018, 108: 650-659.

[11] Anirudhan TS, Parvathy J. Novel thiolated chitosan-polyethyleneglycol blend/montmorillonite composite formulations for the oral delivery of insulin. Bioact Carbohydr Dietary Fibre, 2018, 16: 22-29.

[12] Aranaz I, Mengibar M, Harris R, Paños I, Miralles B, Acosta N, Galed G, Heras Á. Functional characterization of chitin and chitosan. Curr Chem Biol, 2009, 3(2): 203-230.

[13] Arbia W, Arbia L, Adour L, Amrane A. Chitin extraction from crustacean shells using biological methods—a review. Food Technol Biotechnol, 2013, 51(1): 12-25.

[14] Arun Kumar R, Sivashanmugam A, Deepthi S, Iseki S, Chennazhi KP, Nair SV, Jayakumar R. Injectable Chitin-poly (ε-caprolactone)/nanohydroxyapatite composite microgels prepared by simple regeneration technique for bone tissue engineering. ACS Appl Mater Interfaces, 2015, 7: 9399-9409.

[15] Ashry ESHE, Aly MRE. Synthesis and biological relevance of N-acetylglucosamine containing oligosaccharides. Pure Appl Chem, 2007, 12: 2229-2242.

[16] Bano I, Arshad M, Yasin T, Ghauri MA. Preparation, characterization and evaluation of glycerol plasticized chitosan/PVA blends for burn wounds. Int J Biol Macromol, 2019, 124: 155-162.

[17] Barikani M, Oliaei E, Seddiqi H, Honarkar H. Preparation and application of chitin and its derivatives: a review. Iran Polym J, 2014, 23: 307-326.

[18] Benhabiles MS, Salah R, Lounici H, Drouiche N, Goosen MFA, Mameri N. Antibacterial activity of chitin, chitosan and its oligomers prepared from shrimp shell waste. Food Hydrocoll, 2012, 29: 48-56.

[19] Berger J, Reist M, Chenite A, Felt-Baeyens O, Mayer JM, Gurny R. Erratum to Pseudo-thermosetting chitosan hydrogels for biomedical application. Int J Pharm, 2005, 28: 197-206.

[20] Bhowmick A, Pramanik N, Manna PJ, Mitra T, Selvaraj TKR, Gnanamani A, Das M, Kundu PP. Development of porous and antimicrobial CTS–PEG–HAP–ZnO nano-composites for bone tissue engineering. RSC Adv, 2015, 5: 99385-99393.

[21] Birolli WG, Delezuk JADM, Campana-Filho SP. Ultrasound-assisted conversion of alpha-chitin into chitosan. Appl Acoust, 2016, 103: 239-242.

[22] Bohlman JA, Schisler DO, Hwang KO, Hennling JP, Trinkle JR, Anderson TB, Steinke JD, Vanderhoff A (2004) N-Acetyl-d-glucosamine and process for producing N-acetyl-d-glucosamine. US Patent 6,693,188, 17 Feb 2004

[23] Caridade SG, Monge C, Almodóvar J, Guillot R, Lavaud J, Josserand V, Coll JL, Mano JF, Picart C. Myoconductive and osteoinductive free-standing polysaccharide membranes. Acta Biomater, 2015, 15: 139-149.

[24] Cauchie HM. Chitin production by arthropods in the hydrosphere. Hydrobiologia, 2002, 470: 63-96.

[25] Chaput C, Chenite A (2014) Injectable in situ self-forming mineral-polymer hybrid composition and uses thereof. US Patent 8,747,899, 10 Jun 2014

[26] Chen JK, Shen CR, Liu CL. N-Acetylglucosamine: production and applications. Mar Drugs, 2010, 8: 2493-2516.

[27] Cheng MY, Deng JG, Yang F, Gong Y, Zhao N, Zhang X. Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions. Biomaterials, 2003, 24: 2871-2880.

[28] Choorit W, Patthanamanee W, Manurakchinakorn S. Use of response surface method for the determination of demineralization efficiency in fermented shrimp shells. Bioresour Technol, 2008, 99: 6168-6173.

[29] Community Research and Development Information Service, European Commission. http://cordis.europa.eu/home_en.html. Accessed 24 Mar 2018

[30] Dehaghi SM, Rahmanifar B, Moradi AM, Azar PA. Removal of permethrin pesticide from water by chitosan–zinc oxide nanoparticles composite as an adsorbent. J Saudi Chem Soc, 2014, 18(4): 348-355.

[31] Demey H, Vincent T, Guibal E. A novel algal-based sorbent for heavy metal removal. Chem Eng J, 2018, 332: 582-595.

[32] Deng H, Lin P, Li W, Xin S, Zhou X, Yang J. Hydroxypropyl chitosan/organic rectorite-based nanofibrous mats with intercalated structure for bacterial inhibition. J Biomater Sci Polym Ed, 2013, 24(4): 485-496.

[33] Dhillon GS, Kaur S, Brar SK, Verma M. Green synthesis approach: extraction of chitosan from fungus mycelium. Crit Rev Biotechnol, 2013, 33: 379-403.

[34] Dhivya S, Saravanan S, Sastry TP, Selvamurugan N. Nanohydroxyapatite-reinforced chitosan composite hydrogel for bone tissue repair in vitro and in vivo. J Nanobiotechnol, 2015, 40: 1-13.

[35] D’huart JB, Dallas C (2004) Cactaceae-based formulation having the property of fixing fats, and method for obtaining same. US Patent 2004,0,126,444 A1, 1 July, 2004. https://patents.google.com/patent/US20040126444

[36] Ding Y, Shen SZ, Sun H, Sun K, Liu F, Qi Y, Yan J. Design and construction of polymerized-chitosan coated Fe₃O₄ magnetic nanoparticles and its application for hydrophobic drug delivery. Mater Sci Eng C, 2015, 48: 487-498.

[37] D’Mello S, Elangovan S, Hong L, Ross RD, Sumner DR, Salem AK. Incorporation of copper into chitosan scaffolds promotes bone regeneration in rat calvarial defects. J Biomed Mater Res B Appl Biomater, 2015, 103: 1044-1049.

[38] Duan B, Gao H, He M, Zhang L. Hydrophobic modification on surface of chitin sponges for highly effective separation of oil. ACS Appl Mater Interfaces, 2014, 6(22): 19933-19942.

[39] Dutta PK, Dutta J, Tripathi VS (2004) Chitin and chitosan: chemistry, properties and applications. J Sci Ind Res 63:20–31. http://nopr.niscair.res.in/handle/123456789/5397

[40] Dutta J, Tripathi S, Dutta PK. Progress in antimicrobial activities of chitin, chitosan and its oligosaccharides: a systematic study needs for food applications. Food Sci Technol Int, 2012, 18: 3-34.

[41] El-Diasty EM, Nesreen Z, Aideia AM. Using of chitosan as antifungal agent in Kariesh Cheese. New York Sci J, 2012, 5(9): 5-10.

[42] Fan Y, Saito T, Isogai A. TEMPO-mediated oxidation of β-chitin to prepare individual nanofibrils. Carbohydr Polym, 2009, 77(4): 832-838.

[43] Fan Z, Qin Y, Liu S, Xing R, Yu H, Chen X, Li K, Li P. Synthesis, characterization, and antifungal evaluation of diethoxyphosphoryl polyaminoethyl chitosan derivatives. Carbohydr Polym, 2018, 190: 1-11.

[44] Ferreira AR, Alves VD, Coelhoso IM. Polysaccharide-based membranes in food packaging applications. Membranes (Basel), 2016, 6: 2.

[45] Fiamingo A, Augusto J, Delezuk DM, Trombotto S, David L, Campana-filho SP. Ultrasonics sonochemistry extensively deacetylated high molecular weight chitosan from the multistep ultrasound-assisted deacetylation of beta-chitin. Ultrason Sonochem, 2016, 32: 79-81.

[46] Finkielsztein S, Vournakis JN (2015) Hemostatic compositions and therapeutic regimens. US 9,139,664 B2, 22 Sep, 2015. https://www.lens.org/lens/patent/US_9139663_B2

[47] Flores-Albino B, Arias L, Gómez J, Castillo A, Gimeno M, Shirai K. Chitin and L(+)-lactic acid production from crab (Callinectes bellicosus) wastes by fermentation of Lactobacillus sp. B2 using sugar cane molasses as carbon source. Bioprocess Biosyst Eng, 2012, 35(7): 1193-1200.

[48] Francis RJ, Prestwich GD, Hunt G (2015) System and method of delivering a hyaluronic acid composition and a copper composition for treatment of dermatologic conditions. WO 2015,081,304 A1, 4 June 2015. https://patents.google.com/patent/WO2015081304A1/en

[49] Gautier S, Xhauflaire-Uhoda E, Gonry P, Piérard GE. Chitin-704 glucan, a natural cell scaffold for skin moisturization and rejuvenation. Int J Cosmet Sci, 2008, 30: 459-469.

[50] Ghorbel-Bellaaj O, Jellouli K, Younes I, Manni L, Ouled Salem M, Nasri M. A solvent-stable metalloprotease produced by Pseudomonas aeruginosa A2 grown on shrimp shell waste and its application in chitin extraction. Appl Biochem Biotechnol, 2011, 164: 410-425.

[51] Ghorbel-Bellaaj O, Younes I, Maalej H, Hajji S, Nasri M. Chitin extraction from shrimp shell waste using Bacillus bacteria. Int J Biol Macromol, 2012, 51: 1196-1201.

[52] Ghorbel-Bellaaj O, Younes I, Maalej H, Hajji S, Nasri M. Chitin extraction from shrimp shell waste using Bacillus bacteria. Int J Biol Macromol, 2012, 51(5): 1196-1201.

[53] Ghosh P, Rameshbabu AP, Das D, Francis NK, Pawar HS, Subramanian B, Pal S, Dhara S. Covalent cross-links in polyampholytic chitosan fibers enhances bone regeneration in a rabbit model. Colloids Surf B Biointerfaces, 2015, 125: 160-169.

[54] Giteru SG, Ali MA, Oey I. Solvent strength and biopolymer blending effects on physicochemical properties of zein-chitosan-polyvinyl alcohol composite films. Food Hydrocolloids, 2019, 86: 270-286.

[55] Gooday GW. The ecology of chitin degradation. Adv Microb Ecol, 1990, 11: 387-430.

[56] Gortari MC, Hours RA. Biotechnological processes for chitin recovery out of crustacean waste: a mini-review. Electronic J Biotechnol, 2013, 16(3): 14.

[57] Gu Y, Zhu J, Xue C, Li Z, Ding F, Yang Y, Gu X. Chitosan/silk fibroin-based, Schwann cell-derived extracellular matrix-modified scaffolds for bridging rat sciatic nerve gaps. Biomaterials, 2014, 357: 2253-2263.

[58] Guminska M, Ignacak J, Wojcik E. In vitro inhibitory effect of chitosan and its degradation products on energy metabolism in Ehrlich ascites tumour cells (EAT). Pol J Pharmacol, 1996, 48: 495-501.

[59] Hackbarth FV, Girardi F, Santos JC, de Souza AAU, Bonaventura RAR, de Gueli SMA, Souza U, Vilar VJP. Ion-exchange breakthrough curves for single and multi-metal systems using marine macroalgae Pelvetia canaliculata as a natural cation exchanger. Chem Eng J, 2015, 269: 359-370.

[60] Hai TAP, Sugimoto R. Surface modification of chitin and chitosan with poly(3-hexylthiophene) via oxidative polymerization. Appl Surf Sci, 2018, 434: 188-197.

[61] Hajji S, Younes I, Ghorbel-Bellaaj O, Hajji R, Rinaudo M, Nasri M, Jellouli K. Structural differences between chitin and chitosan extracted from three different marine sources. Int J Biol Macromol, 2014, 65: 298-306.

[62] Han F, Dong Y, Su Z, Yin R, Song A, Li S. Preparation, characteristics and assessment of a novel gelatin–chitosan sponge scaffold as skin tissue engineering material. Int J Pharm, 2014, 476: 124-133.

[63] Hu FQ, Ren GF, Yuan H, Du YZ, Zeng S. Shell cross-linked stearic acid grafted chitosan oligosaccharide self-aggregated micelles for controlled release of paclitaxel. Colloids Surf B, 2006, 50: 97-103.

[64] Hu Z, Zhang J, Chan W, Szeto Y. The sorption of acid dye onto chitosan nanoparticles. Polymer, 2006, 47(16): 5838-5842.

[65] Hu FQ, Liu LN, Du YZ, Yuan H. Synthesis and antitumor activity of doxorubicin conjugated stearic acid-g-chitosan oligosaccharide polymeric micelles. Biomaterials, 2009, 30: 6955-6963.

[66] Huang Y, He M, Lu A, Zhou W, Stoyanov SD, Pelan EG, Zhang L. Hydrophobic modification of chitin whisker and its potential application in structuring oil. Langmuir, 2015, 31(5): 1641-1648.

[67] Ifuku S, Shervani Z, Saimoto H. Thomas S, Pothan LA. Preparation of chitin nanofibers and their composites. Biopolymer nanocomposites: processing, properties, and applications, 2013, Hoboken: Wiley.

[68] Iqbal P, Preece JA, Mendes PM. Steed JW, Gale PA. Nanotechnology: the “Top-Down” and “Bottom-Up” approaches. Supramolecular chemistry: from molecules to nanomaterials, 2012, Oxford: Wiley.

[69] Islam MS, Khan S, Tanaka M. Waste loading in shrimp and fish processing effluents: potential source of hazards to the coastal and near shore environments. Mar Pollut Bull, 2004, 49: 103-110.

[70] Jeon YJ, Kim SK. Antitumor activity of chitosan oligosaccharides produced in ultrafiltration membrane reactor system. J Microbiol Biotechnol, 2002, 12(3): 503-507.

[71] Ji J, Tong X, Huang X, Wang T, Lin Z, Cao Y, Zhang J, Dong L, Qin H, Hu Q. Sphere-shaped nano-hydroxyapatite/chitosan/gelatin 3D porous scaffolds increase proliferation and osteogenic differentiation of human induced pluripotent stem cells from gingival fibroblasts. Biomed Mater, 2015, 10: 045005.

[72] Jiang T, Abdel-Fattah W, Laurencin CT. In vitro evaluation of chitosan/poly (lactic acid-glycolic acid) sintered microsphere scaffolds for bone tissue engineering. Biomaterials, 2006, 27: 4894-4903.

[73] João CFC, Silva JC, Borges JP. Chitin-based nanocomposites: biomedical applications. Eco-friendly polymer nanocomposites, 2015, New Delhi: Springer, 439-457.

[74] Johnson EL, Nichols EJ (2000) High tap density chitosan, and methods of production. US 6130321 A, Oct

[75] Kadokawa JI. Fabrication of nanostructured and microstructured chitin materials through gelation with suitable dispersion media. RSC Adv, 2015, 5(17): 12736-12746.

[76] Kaur S, Dhillon GS. The versatile biopolymer chitosan: potential sources, evaluation of extraction methods and applications. Crit Rev Microbiol, 2014, 40(2): 155-175.

[77] Kaur S, Dhillon GS. Recent trends in biological extraction of chitin from marine shell wastes: a review. Crit Rev Biotechnol, 2015, 35: 44-61.

[78] Keeney M, Han LH, Onyiah S, Yang F. Rosen Y, Elman N. Tissue engineering: focus on the musculoskeletal system. Biomaterial science: an integrated clinical and engineering approach, 2012, New York: CRC-Press, 191-221.

[79] Khanafari A, Marandi R, Sanatei S. Recovery of chitin and chitosan from shrimp waste by chemical and microbial methods. Iran J Environ Health Sci Eng, 2008, 5(1): 19-24.

[80] Khorrami M, Najafpour GD, Younesi H, Amini GH. Growth kinetics and demineralization of shrimp shell using Lactobacillus plantarum PTCC 1058 on various carbon sources. Iran J Energy Environ, 2011, 2(4): 320-325.

[81] Khorrami M, Najafpour GD, Younes H, Hosseinpour MN (2012) Production of chitin and chitosan from shrimp shell in batch culture of Lactobacillus plantarum. Chem Biochem Eng Quarterly 26(3):217–223. https://hrcak.srce.hr/87355

[82] Kim YH, Gihm SH, Park CR, Lee KY, Kim TW, Kwon IC, Chung H, Jeong SY. Structural characteristics of size-controlled self-aggregates of deoxycholic acid-modified chitosan and their application as a DNA delivery carrier. Bioconjugate Chem, 2001, 12: 932-938.

[83] Knidri HE, Khalfaouy RE, Laajeb A, Addaou A, Lahsini A. Eco-friendly extraction and characterization of chitin and chitosan from the shrimp shell waste via microwave irradiation. Process Saf Environ Prot, 2016, 104: 395-405.

[84] Kwok KC, Koong LF, Chen G, McKay G. Mechanism of arsenic removal using chitosan and nanochitosan. J Colloid Interface Sci, 2014, 416: 1-10.

[85] Kyzas GZ, Siafaka PI, Pavlidou EG, Chrissafis KJ, Bikiaris DN. Synthesis and adsorption application of succinyl-grafted chitosan for the simultaneous removal of zinc and cationic dye from binary hazardous mixtures. Chem Eng J, 2015, 259: 438-448.

[86] Lai GJ, Shalumon KT, Chen JP. Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds. Int J Nanomed, 2015, 10: 567-584.

[87] Lee EJ, Kim HE. Accelerated bony defect healing by chitosan/silica hybrid membrane with localized bone morphogenetic protein-2 delivery. Mater Sci Eng C, 2016, 59: 339-345.

[88] Lee EA, Pan CH, Son JM, Kim SI. Isolation and characterization of basic exochitinase from leaf extract of Rehmannia glutinosa. Biosci Biotechnol Biochem, 1999, 63: 1781-1783.

[89] Levengood SL, Zhang M. Chitosan-based scaffolds for bone tissue engineering. J Mater Chemi B Mater Biol Med, 2014, 2: 3161-3184.

[90] Li F, Zhang X, Li H, Xiang L, Chen Y. Preparation of self-assembled nanoparticles of chitosan oligosaccharide-graftpolycaprolactone as a carrier of bovine serum albumin drug. Biomed Mater Eng, 2014, 24: 2041-2048.

[91] Liu D, Chang PR, Chen M, Wu Q. Chitosan colloidal suspension composed of mechanically disassembled nanofibers. J Colloid Interface Sci, 2011, 354(2): 637-643.

[92] Liu D, Wu Q, Chang PR, Gao G. Self-assembled liquid crystal film from mechanically defibrillated chitosan nanofibers. Carbohydr Polym, 2011, 84(1): 686-689.

[93] Lucchesi L, Xie H (2015) Wound dressing devices and methods. US 9,205,170 B2, 8 Dec 2015. https://patents.google.com/patent/US9205170

[94] Ma J, Xin C, Tan C. Preparation, physicochemical and pharmaceutical characterization of chitosan from Catharsius molossus residue. Int J Biol Macromol, 2015, 80: 547-556.

[95] Mahmoud NS, Ghaly AE, Arab F. Unconventional approach for demineralization of deproteinized crustacean shells for chitin production. Am J Biochem Biotechnol, 2007, 3: 1-9.

[96] Marciello M, Rossi S, Caramella C, Remuñán-López C. Freeze-dried cylinders carrying chitosan nanoparticles for vaginal peptide delivery. Carbohydr Polym, 2017, 170: 43-51.

[97] Masuda S, Azuma K, Kurozumi S, Kiyose M, Osaki T, Tsuka T, Itoh N, Imagawa T, Minami S, Sato K, Okamoto Y. Anti-tumor properties of orally administered glucosamine and N acetyl-d-glucosamine oligomers in a mouse model. Carbohydr Polym, 2014, 111: 783-787.

[98] McCarthy S, McGrath B, Winata E (2012) Absorbable tissue dressing assemblies, systems, and methods formed from hydrophilic polymer sponge structures such as chitosan. US 8,269,058 B2, 18 Sept 2012. https://www.lens.org/lens/patent/US_8269058_B2

[99] McCarthy SJ, Gregory KW, Wiesmann WP, Campbell TD (2014) Wound dressing and method for controlling severe, life-threatening bleeding. US 8,668,924 B2, 11 Mar 2014. https://patents.google.com/patent/US8668924

[100] Medovent Reaxon^® http://medovent.de/en/reaxon/reaxon-nerve-guide/. Accessed 24 Mar 2018

[101] Merzendorfer H, Zimoch L. Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol, 2003, 206: 4393-4412.

[102] Mincea M, Negrulescu A, Ostafe V. Preparation, modification, and applications of chitin nanowhiskers: a review. Rev Adv Mater Sci, 2012, 30(3): 225-242.

[103] Moatarya A, Teimouria A, Bagherzadeh M, Chermahini AN, Razavizadeh R. Design and fabrication of novel chitin hydrogel/chitosan/nano diopside, composite scaffolds for tissue engineering. Ceram Int, 2017, 43: 1657-1668.

[104] Mohammed MH, Williams PA, Tverezovskaya O. Extraction of chitin from prawn shells and conversion to low molecular mass chitosan. Food Hydrocoll, 2013, 31: 166-171.

[105] Morganti P. Innovation, nanotechnology and industrial sustainability by the use of natural underutilized byproducts. J Mol Biochem, 2013, 2(3): 137-141.

[106] Morganti P, Fabrizi G, Palombo P, Palombo M, Ruocco E, Cardillo A, Morganti G. Chitin-nanofibrils: a new active cosmetic carrier. J Appl Cosmetol, 2008, 26(3): 113-128.

[107] Morganti P, Del Ciotto P, Carezzi F, Morganti G, Hong-Duo C. From waste material a new anti-aging compound: a chitin nanofiber complex. SOFW J, 2012, 138(7): 30-36.

[108] Nasrin R, Biswas S, Rashid TU, Afrin S, Jahan RA, Haque P, Rahman MM. Preparation of chitin-PLA laminated composite for implantable application. Bioactive Mater, 2017, 2: 199-207.

[109] Nishimura S, Nishi N, Tokura S, Nishimura K, Azuma I. Bioactive chitin derivatives. Activation of mouse-peritoneal macrophages by O (carboxymethyl) chitins. Carbohydr Res, 1986, 146: 251-258.

[110] Olivera S, Muralidhara HB, Venkatesh K, Guna VK, Gopalakrishna K, Kumar Y. Potential applications of cellulose and chitosan nanoparticles/composites in wastewater treatment: a review. Carbohydr Polym, 2016, 153: 600-618.

[111] Pachapur VL, Guemiza K, Rouissi T, Sarma SJ, Brar SK. Novel biological and chemical methods of chitin extraction from crustacean waste using saline water. J Chem Technol Biotechnol, 2016, 91(8): 2331-2339.

[112] Pal P, Pal A. Surfactant-modified chitosan beads for cadmium adsorption. Int J Biol Macromol, 2017, 14: 1548-1555.

[113] Pangon A, Saesoo S, Saengkrit N, Ruktanonchai U, Intasanta V. Hydroxyapatite-hybridized chitosan/chitin whisker bionanocomposite fibers for bone tissue engineering applications. Carbohydr Polym, 2016, 144: 419-427.

[114] Park BK, Kim MM. Applications of chitin and its derivatives in biological medicine. Int J Mol Sci, 2010, 11: 5152-5164.

[115] Pearson RG, Molino Y, Williams PM, Tendler SJ, Davies MC, Roberts CJ, Shakesheff KM. Spatial confinement of neurite regrowth from dorsal root ganglia within nonporous microconduits. Tissue Eng, 2003, 9: 201-208.

[116] Percot A, Viton C, Domard A. Characterization of shrimp shell deproteinization. Biomacromol, 2003, 4: 1380-1385.

[117] Pfister LA, Papaloizos M, Merkle HP, Gander B. Hydrogel nerve conduits produced from alginate/chitosan complexes. J Biomed Mater Res A, 2007, 80: 932-937.

[118] Philibert T, Lee BH, Fabien N. Current status and new perspectives on chitin and chitosan as functional biopolymers. Appl Biochem Biotechnol, 2017, 181: 1314-1337.

[119] Pourhaghgouy M, Zamanian A, Shahrezaee M, Masouleh MP. Physicochemical properties and bioactivity of freeze-cast chitosan nanocomposite scaffolds reinforced with bioactive glass. Mater Sci Eng C, 2016, 58: 180-186.

[120] Prameela K, Murali Mohan C, Smitha PV, Hemalatha KPJ. Bioremediation of shrimp biowaste by using natural probiotic for chitin and carotenoid production an alternative method to hazardous chemical method. Int Appl Biol Pharm, 2010, 1(3): 903-910.

[121] Pyrzynska K. Removal of cadmium from wastewaters with low-cost adsorbents. J Environ Chem Eng, 2019, 7(1): 102795.

[122] Qi L, Xu Z, Jiang X, Hu C, Zou X. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res, 2004, 339(16): 2693-2700.

[123] Quiñones JP, Peniche H, Peniche C. Chitosan based self-assembled nanoparticles in drug delivery. Polymers, 2018, 10: 235.

[124] Raja MA, Zeenat S, Arif M, Liu C. Self-assembled nanoparticles based on amphiphilic chitosan derivative and arginine for oral curcumin delivery. Int J Nanomed, 2016, 11: 4397-4412.

[125] Rajendran R, Abirami M, Prabhavathi P, Premasudha P, Kanimozhi B, Manikandan A. Biological treatment of drinking water by chitosan based nanocomposites. Afr J Biotechnol, 2015, 14(11): 930-936.

[126] Ramírez MA, Rodriguez AT, Alfonso L, Peniche C. Chitin and its derivatives as biopolymers with potential agricultural applications. Biotecnol Aplicada, 2010, 27(4): 270-276.

[127] Rao MB, Tanksale AM, Ghatge MS, Deshpande VV. Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev, 1998, 62: 597-635.

[128] Rege PR, Block LH. Chitosan processing: influence of process parameters during acidic and alkaline hydrolysis and effect of the processing sequence on the resultant chitosan’s properties. Carbohydr Res, 1999, 321: 235-245.

[129] Rezakazemi M, Albadarin AB, Walker GM, Shirazian S. Quantum chemical calculations and molecular modeling for methylene blue removal from water by a lignin–chitosan blend. Int J Biol Macromol, 2018, 120B: 2065-2075.

[130] Ribeiro JCV, Vieira RS, Melo IM, Araújo VMA, Lima V. Versatility of chitosan-based biomaterials and their use as scaffolds for tissue regeneration. Sci World J, 2017, 2017: 8639898.

[131] Sarkar SD, Farrugia BL, Dargaville TR, Dhara S. Chitosan-collagen scaffolds with nano/microfibrous architecture for skin tissue engineering. J Biomed Mater Res A, 2013, 101: 3482-3492.

[132] Sashiwa H, Fujishima S, Yamano N, Kawasaki N, Nakayama A, Muraki E, Aiba S. Production of N-acetyl-d-glucosamine from β-Chitin by enzymatic hydrolysis. Chem Lett, 2001, 31: 308-309.

[133] Sedaghat F, Yousefzadi M, Toiserkani H, Najafipour S. Bioconversion of shrimp waste Penaeus merguiensis using lactic acid fermentation: an alternative procedure for chemical extraction of chitin and chitosan. Int J Biol Macromol, 2017, 104A: 883-888.

[134] Sethulekshmi C. Chitin and its benefits. Int J Adv Res Biol Sci, 2014, 1(6): 171-175.

[135] Seyedi SM, Anvaripour B, Motavassel M, Jadidi N. Comparative cadmium adsorption from water by nanochitosan and chitosan. Int J Eng Innovaive Technol, 2013, 2(9): 145-148.

[136] Shakir M, Jolly R, Khan MS, Iram N, Khan HM. Nano hydroxyapatite/chitosan starch nanocomposite as a novel bone construct: synthesis and in vitro studies. Int J Biol Macromol, 2015, 80: 282-292.

[137] Shalumon KT, Lai GJ, Chen CH, Chen JP. Modulation of bone-specific tissue regeneration by incorporating bone morphogenetic protein and controlling the shell thickness of silk fibroin/chitosan/nanohydroxyapatite core-shell nanofibrous membranes. ACS Appl Mater Interfaces, 2015, 7: 21170-21181.

[138] Shariatinia Z. Pharmaceutical applications of chitosan. Adv Colloid Interface Sci, 2019, 263: 131-194.

[139] Shekhawat A, Kahu S, Saravanan D, Jugade R. Removal of Hg(II) and Cd(II) by ionic solid impregnated chitosan. Int J Biol Macromol, 2017, 104: 1556-1568.

[140] Sinha S, Chand S, Tripathi P. Microbial degradation of chitin waste for production of chitosanase and food related bioactive compounds. Appl Biochem Microbiol, 2014, 50(2): 125-133.

[141] Sini TK, Santhosh S, Mathew PT. Study on the production of chitin and chitosan from shrimp shell by using Bacillus subtilis fermentation. Carbohydr Res, 2007, 342: 2423-2429.

[142] Sivakami M, Gomathi T, Venkatesan J, Jeong H-S, Kim S-K, Sudha P. Preparation and characterization of nano chitosan for treatment wastewaters. Int J Biol Macromol, 2013, 57: 204-212.

[143] Srinivasan H, Velayutham K, Ravichandran R. Chitin and chitosan preparation from shrimp shells Penaeus monodon and its human ovarian cancer cell line, PA-1. Int J Biol Macromol, 2018, 107A: 662-667.

[144] Sun X, Zhu J, Gu Q, You Y. Surface-modified chitin by TEMPO-mediated oxidation and adsorption of Cd(II). Colloids Surfaces A Physicochem Eng Aspects, 2018, 555: 103-110.

[145] Synowiecki J, Al-Khateeb NAAQ. The recovery of protein hydrolysate during enzymatic isolation of chitin from shrimp Crangon crangon processing discards. Food Chem, 2000, 68: 147-152.

[146] Tanaka K, Yamamoto K, Kadokawa J. Facile nanofibrillation of chitin derivatives by gas bubbling and ultrasonic treatments in water. Carbohydr Res, 2014, 398: 25-30.

[147] Tokoro A, Tatewaki N, Suzuki K, Mikami T, Suzuki S, Suzuki M. Growth inhibitory effect of hexa N-acetylchitohexaose and chitohexaose against Meth-A solid tumor. Chem Pharm Bull, 1988, 36: 784-790.

[148] Tokuyasu K, Mitsutomi M, Yamaguchi I, Hayashi K, Mori Y. Recognition of chitooligosaccharides and their N-acetyl groups by putative subsites of chitin deacetylase from a deuteromycete, Colletotrichum lindemuthianum. Biochemistry, 2000, 39: 8837-8843.

[149] Tsaih ML, Chen RH. The effect of reaction time and temperature during heterogenous alkali deacetylation on degree of deacetylation and molecular weight of resulting chitosan. J Appl Polym Sci, 2003, 88: 2917-2923.

[150] Tu H, Yu Y, Chen J, Shi X, Zhou J, Deng H, Du Y. Highly cost-effective and high-strength hydrogels as dye adsorbents from natural polymers: chitosan and cellulose. Polym Chem, 2017, 8: 2913-2921.

[151] Usman A, Zia KM, Zuber M, Tabasum S, Rehman S, Zia F. Chitin and chitosan based polyurethanes: a review of recent advances and prospective biomedical applications. Int J Biol Macromol, 2016, 86: 630-645.

[152] Viarsagh MS, Janmaleki M, Falahatpisheh HR, Masoumi J. Chitosan preparation from Persian gulf shrimp shells and investigating the effect of time on the degree of deacetylation. J Paramed Sci, 2010, 1: 2.

[153] Wang YS, Liu LR, Jiang Q, Zhang QQ. Self-aggregated nanoparticles of cholesterol-modified chitosan conjugate as a novel carrier of epirubicin. Eur Polym J, 2007, 43: 43-51.

[154] Wang Y, Zhang LH, Hu M, Liu HC, Wen WS, Xiao HX, Niu Y. Synthesis and characterization of collagen-chitosan-hydroxyapatite artificial bone matrix. J Biomed Mater Res A, 2008, 86(1): 244-252.

[155] Wang XM, Zhang J, Chen H, Wang Q. Preparation and characterization of collagen-based composite conduit for peripheral nerve regeneration. J Appl Polym Sci, 2009, 112: 3652-3662.

[156] Wang X, You C, Hu X, Zheng Y, Li Q, Feng Z, Sun H, Gao C, Han C. The roles of knitted mesh-reinforced collagen-chitosan hybrid scaffold in the one-step repair of full-thickness skin defects in rats. Acta Biomater, 2013, 9: 7822-7832.

[157] Wang X, Wu P, Hu X, You C, Guo R, Shi H, Guo S, Zhou H, Chaoheng Y, Zhang Y, Han C. Polyurethane membrane/knitted mesh-reinforced collagen–chitosan bilayer dermal substitute for the repair of full-thickness skin defects via a two-step procedure. J Mech Behav Biomed Mater, 2016, 56: 120-133.

[158] Wijesena RN, Tissera N, Kannangara YY, Lin Y, Amaratunga GAJ, Silva KMN. A method for top down preparation of chitosan nanoparticles and nanofibers. Carbohydr Polym, 2015, 117: 731-738.

[159] Xie J, Peng C, Zhao Q, Wang X, Yuan H, Yang L, Li K, Lou X, Zhang Y. Osteogenic differentiation and bone regeneration of iPSC-MSCs supported by a biomimetic nanofibrous scaffold. Acta Biomater, 2016, 29: 365-379.

[160] Xu Y, Bajaj M, Schneider R, Grage SL, Ulrich AS, Winter J, Gallert C. Transformation of the matrix structure of shrimp shells during bacterial deproteination and demineralization. Microb Cell Fact, 2013, 12: 90.

[161] Yan D, Hu S, Zhou Z, Zeenat S, Cheng F, Li Y, Feng C, Cheng X, Cheng X. Different chemical groups modification on the surface of chitosan nonwoven dressing and the hemostatic properties. Int J Biol Macromol, 2018, 107A: 463-469.

[162] Yan JK, Qiu WY, Wang YY, Wu LX, Cheung PCK. Formation and characterization of polyelectrolyte complex synthesized by chitosan and carboxylic curdlan for 5-fluorouracil delivery. Int J Biol Macromol, 2018, 107: 397-405.

[163] Yang Y, Gu X, Tan R, Hu W, Wang X, Zhang P, Zhang T. Fabrication and properties of a porous chitin/chitosan conduit for nerve regeneration. Biotechnol Lett, 2004, 26: 1793-1797.

[164] Yang YM, Wu J, Wang XD, Liu J, Ding F, Gu XS. Fabrication and evaluation of chitin-based nerve guidance conduits used to promote peripheral nerve regeneration. Adv Eng Mater, 2009, 11: B209-B218.

[165] Yin M, Lin X, Ren T, Li Z, Ren X, Huang TS. Cytocompatible quaternized carboxymethyl chitosan/poly(vinyl alcohol) blend film loaded copper for antibacterial application. Int J Biol Macromol, 2018, 120A: 992-998.

[166] Yong C, Bond Y, Hui C, Talib A. Extraction and physicochemical characterization of chitin and chitosan from Zophobas morio larvae in varying sodium hydroxide concentration. Int J Biol Macromol, 2018, 108: 135-142.

[167] Younes I, Rinaudo M. Chitin and chitosan preparation from marine sources. Structure, properties and applications. Mar Drugs, 2015, 13: 1133-1174.

[168] Younes I, Ghorbel-Bellaaj O, Nasri R, Chaabouni M, Rinaudo M, Nasri M. Chitin and chitosan preparation from shrimp shells using optimized enzymatic deproteinization. Process Biochem, 2012, 47(12): 2032-2039.

[169] Zhang L, Zeng Y, Cheng Z. Removal of heavy metal ions using chitosan and modified chitosan: a review. J Mol Liq, 2016, 214: 175-191.

[170] Zhao Y, Park RD, Muzzarelli RAA. Chitin deacetylases: properties and applications. Mar Drugs, 2010, 8: 24-46.

[171] Zhou C, Wu Q. A novel polyacrylamide nanocomposite hydrogel reinforced with natural chitosan nanofibers. Colloids Surf B Biointerfaces, 2011, 84(1): 155-162.

Funding

Department of Biotechnology, Ministry of Science and Technology(BT/RLF/Re-entry/04/2013)