Bioremediation of brewery wastewater using hydroponics planted with vetiver grass in Addis Ababa, Ethiopia

Abstract

Background

Bioremediation is the use of biological interventions for mitigation of the noxious effects caused by pollutants in the environment including wastewater. It is very useful approach for a variety of applications in the area of environmental protection. It has become an attractive alternative to the conventional cleanup technologies that employ plants and their associated microorganisms to remove, contain, or render harmless environmental contaminants.

Methods

Three parallel hydroponic treatment systems (each 2 m long × 0.75 m wide × 0.65 m deep) and one control unit were filled with brewery wastewater to an effective depth of 0.5 m. Two sets of floating polystyrene platform were used for each treatment unit to support vetiver tillers for conducting bioremediation study. The wastewater was fed to the hydroponic treatment units at hydraulic loading rate of 10 cm d⁻¹ and hydraulic residence time of 5 days. Influent and effluent samples were collected once a month for 7 months, and analyzed to determine the various parameters relating to the water quality including plant growth and nutrient analyses.

Results

Vetiver grass grew and established with well-developed root and shoots in the hydroponics under fluctuations of brewery wastewater loads and showed phytoremedial capacity to remove pollutants. Removal efficiencies for BOD₅ and COD were significant (p < 0.05), up to 73% (748–1642 mg l⁻¹ inlet), and up to 58% (835–2602 mg l⁻¹ inlet), respectively. Significant removal efficiencies (p < 0.05) ranged from 26 to 46% (14–21 mg l⁻¹ inlet) for TKN, 28–46% (13–19 mg l⁻¹ inlet) for NH₄ ⁺-N, 35–58% (4–11 mg l⁻¹ inlet) for NO₃ ⁻-N, and 42–63% (4–8 mg l⁻¹ inlet) for PO₄ ⁻³-P were recorded. Nutrient accumulation in the samples harvested were varied between 7.4 and 8.3 g N kg⁻¹ dry weight and 6.4–7.5 g P kg⁻¹ dry weight in the hydroponic treatment units during the study period.

Conclusions

This study has shown suitability of vetiver grass for organics and nutrient removal in the bioremediation of brewery wastewater using hydroponics technique in addition to production of valuable biomass. Bioremediation using hydroponics is green and environmentally sustainable approach that offers promising alternative for wastewater treatment in developing countries including Ethiopia.

Keywords

Bioremediation / Brewery wastewater / Hydroponics / Nutrients / Organics / Vetiver grass

References

[1] Akbarzadeh A, Jamshidi S, Vakhshouri M. Nutrient uptake rate and removal efficiency of Vetiveria zizanioides in contaminated waters. Pollution, 2015, 1(1): 1-8.

[2] Akratos CS, Tsihrintzis VA. Effect of temperature, HRT, vegetation and porous media on removal efficiency of pilot-scale horizontal subsurface flow constructed wetlands. Ecol Eng, 2007, 29(2): 173-191.

[3] Alayu E, Yirgu Z. Advanced technologies for the treatment of wastewaters from agro-processing industries and cogeneration of by-products: a case of slaughterhouse, dairy and beverage industries. Int J Environ Sci Technol, 2017

[4] APHA. Standard methods for the examination of water and wastewater, 1999, 20, Washington, DC: American Public Health Association (APHA), American Water Works Association, Water Environment Federation.

[5] Ayaz SC, Akça L. Treatment of wastewater by natural systems. Environ Int, 2001, 26(3): 89-195.

[6] Bindu T, Sylas V, Mahesh M, Rakesh P, Ramasamy E. Pollutant removal from domestic wastewater with Taro (Colocasia esculenta) planted in a subsurface flow system. Ecol Eng, 2008, 33(1): 68-82.

[7] Boonsong K, Chansiri M (2007) Efficiency of vetiver grass cultivated with floating platform technique in domestic wastewater treatment. Chulalongkorn University, Phyathai Rd., Bangkok 10330

[8] Boonsong K, Chansiri M. Domestic wastewater treatment using vetiver grass cultivated with floating platform technique. AU J T, 2008, 12(2): 73-80.

[9] Brisson J, Chazarenc F. Maximizing pollutant removal in constructed wetlands: should we pay more attention to macrophyte species selection?. Sci Total Environ, 2009, 407(13): 3923-3930.

[10] Calheiros CS, Rangel AO, Castro PM. Treatment of industrial wastewater with two-stage constructed wetlands planted with Typha latifolia and Phragmites australis. Bioresource Technol, 2009, 100(13): 3205-3213.

[11] Chen T, Kao C, Yeh T, Chien H, Chao A. Application of a constructed wetland for industrial wastewater treatment: a pilot-scale study. Chemosphere, 2006, 64(3): 497-502.

[12] Darajeh N, Idris A, Aziz AA, Truong P (2014) Vetiver system technology for phytoremediation of palm oil mill effluent (POME). In: 1st Philippine conference on vetiver, Manila, 2014

[13] Darajeh N, Idris A, Truong P, Abdul Aziz A, Abu Bakar R, Che Man H. Phytoremediation potential of vetiver system technology for improving the quality of palm oil mill effluent. Adv Mater Sci Eng, 2014

[14] Driessen W, Vereijeken T (2003) Recent developments in biological treatment of brewery effluent. In: Paper presented to the Institute and Guide of brewery convention, Livingstone, Zambia

[15] Environmental Protection Authority (EPA) in Collaboration with the United Nations Industrial Development Organization (2003) Provisional standards for industrial pollution control in Ethiopia, Addis Ababa

[16] Fillaudeau L, Blanpain-Avet P, Daufin G. Water, wastewater and waste management in brewing industries. J Clean Prod, 2006, 14(5): 463-471.

[17] Gnansounou E, Alves CM, Raman JK. Multiple applications of vetiver grass—a review. Int J Environ Sci, 2017, 2: 125-141.

[18] Haddad M, Mizyed Nm, Abdallah A (2009) Performance of hydroponic system as decentralized wastewater treatment and reuse for rural communities, pp. 91–98, Nablus

[19] Hart B, Cody R, Truong P (2003) Hydroponic vetiver treatment of post septic tank effluent. In: International Conference on Vetiver and Exhibition, 2003

[20] Headley T, Tanner C (2008) Floating treatment wetlands: an innovative option for stormwater quality applications. In: 11th International conference on wetland systems for water pollution control, 2008

[21] Hinchman RR, Negri MC, Gatliff EG (1996) Phytoremediation: using green plants to clean up contaminated soil, groundwater and wastewater. In: Proceedings of International Topical Meeting on Nuclear and Hazardous Waste Management, Spectrum, 1996

[22] Jaiyeola AT, Bwapwa JK. Treatment technology for brewery wastewater in a water-scarce country: a review. S Af J Sci, 2016, 112(3–4): 1-8.

[23] Jamshidi S, Akbarzadeh A, Woo K-S, Valipour A. Wastewater treatment using integrated anaerobic baffled reactor and Bio-rack wetland planted with Phragmites sp. and Typha sp. J Environ Health Sci Eng, 2014, 12(1): 1-12.

[24] Joshua OO, Kehinde AI. Life cycle assessment and management of water use in selected breweries in Nigeria. Civil Eng Archit, 2014, 2(5): 191-200.

[25] Kouki S, Saidi N, Rajeb AB, M’hiri F. Performances of a constructed wetland treating domestic wastewaters during a macrophytes life cycle. Desalination, 2009, 246: 452-467.

[26] Lemji HH, Eckstädt H. Efficiency of a pilot scale trickling filter to treat industrial brewery wastewater: influence of hydraulic loading. J Chem Technol Biotechnol, 2015, 90(1): 201-207.

[27] López-Chuken UJ. Hydroponics-a standard methodology for plant biological researches, 2012, Croatia: InTech press, 181-198.

[28] Mathew M, Sebastian M, Cherian SM (2016) Effectiveness of vetiver system for the treatment of wastewater from an institutional kitchen. In: Procedia Technology, International Conference on Emerging Trends in Engineering, Science and Technology (ICETEST-2015)

[29] Mavrogianopoulos G, Vogli V, Kyritsis S. Use of wastewater as a nutrient solution in a closed gravel hydroponic culture of giant reed (Arundo donax). Bioresource Technol, 2002, 82(2): 103-107.

[30] Minh Tran T, Lacoursière JO, Vought L, Thanh Doan P, Van Tran M (2015) Capacity of Vitiver grass in treatment of a mixture of laboratory and domestic wastewaters. In: 6th International Conference on Vetiver (ICV6), Da Nang, Vietnam, 2015

[31] Nebyou S (2011) Technical efficiency analysis of the Ethiopian brewery. Dissertation, Addis Ababa University, Ethiopia industry

[32] Norton, S  (2014) Removal mechanisms in constructed wastewater wetlands. http://Home.Eng.Iastate.Edu/Tge/Ce421-521/Stephen.  Accessed 31 Mar 2018

[33] Ojoawo SO, Udayakumar G, Naik P. Phytoremediation of phosphorus and nitrogen with canna x generalis reeds in domestic wastewater through NMAMIT constructed wetland. Aquatic Procedia, 2015, 4: 349-356.

[34] Olajire AA. The brewing industry and environmental challenges. J Clean Prod, 2012

[35] Osem Y, Chen Y, Levinson D, Hadar Y. The effects of plant roots on microbial community structure in aerated wastewater-treatment reactors. Ecol Eng, 2007, 29(2): 133-142.

[36] Papadopoulos II, Chatzitheodoridis F, Papadopoulos C, Tarelidis V, Gianneli C. Evaluation of hydroponic production of vegetables and ornamental pot-plants in a heated greenhouse in Western Macedonia, Greece. Am J Agric Biol Sci, 2008, 3(3): 559-565.

[37] Paz-Alberto AM, Sigua GC. Phytoremediation: a green technology to remove environmental pollutants. Am J Climate Change, 2013, 2(01): 1-86.

[38] Pettigrew L, Blomenhofer V, Hubert S, Groß F, Delgado A. Optimisation of water usage in a brewery clean-in-place system using reference nets. J Clean Prod, 2015, 87: 583-593.

[39] Roongtanakiat N, Tangruangkiat S, Meesat R. Utilization of vetiver grass (Vetiveria zizanioides) for removal of heavy metals from industrial wastewaters. Sci Asia, 2007, 33: 397-403.

[40] Shah M, Hashmi HN, Ali A, Ghumman AR. Performance assessment of aquatic macrophytes for treatment of municipal wastewater. J Environ Health Sci Eng, 2014, 12: 106.

[41] Simate GS. The treatment of brewery wastewater for reuse by integration of coagulation/flocculation and sedimentation with carbon nanotubes ‘sandwiched’ in a granular filter bed. J Ind Eng Chem, 2015, 21: 1277-1285.

[42] Stottmeister U, Wießner A, Kuschk P, Kappelmeyer U, Kästner M, Bederski O, Müller R, Moormann H. Effects of plants and microorganisms in constructed wetlands for wastewater treatment. Biotechnol Adv, 2003, 22(1): 93-117.

[43] Tao W, Hall KJ, Duff SJ. Performance evaluation and effects of hydraulic retention time and mass loading rate on treatment of woodwaste leachate in surface-flow constructed wetlands. Ecol Eng, 2006, 26(3): 252-265.

[44] UNEP (2008) Sustainable water utilization in African breweries: current practices and prospects. Report prepared for the UNEP project on African Brewery Sector Water Saving Initiative (ABREW)

[45] United states Environment Protection Agency (USEPA) (2000) Constructed wetlands treatment of municipal wastewaters. United States Environmental Protection Agency, EPA/625/R-99/010, Cincinnati, Ohio, USA., U.S. Environmental Protection Agency USA

[46] Valipour A, Raman VK, Ahn Y-H. Effectiveness of domestic wastewater treatment using a bio-hedge water hyacinth wetland system. Water, 2015, 7(1): 329-347.

[47] Van Rooijen DJ, Biggs TW, Smout I, Drechsel P. Urban growth, wastewater production and use in irrigated agriculture: a comparative study of Accra, Addis Ababa and Hyderabad. Irrig Drain Syst, 2010, 24(1–2): 53-64.

[48] Vipat V, Singh U, Billore S (2008) Efficacy of rootzone technology for treatment of domestic wastewater: Field Scale Study of a Pilot Project in Bhopal (MP), India. In: The 12th world lake conference, 2008

[49] Vymazal J. Removal of nutrients in various types of constructed wetlands. Sci Total Environ, 2007, 380(1): 48-65.

[50] Vymazal J. Constructed wetlands for wastewater treatment. Water, 2010, 2(3): 530-549.

[51] Vymazal J. Plants used in constructed wetlands with horizontal subsurface flow: a review. Hydrobiologia, 2011, 674(1): 133-156.

[52] Vymazal J, Kröpfelová L. Wastewater treatment in constructed wetlands with horizontal subsurface flow, 2008, Kamýcká, Czech Republic: Springer

[53] Wu H, Zhang J, Ngo HH, Guo W, Hu Z, Liang S, Fan J, Liu H. A review on the sustainability of constructed wetlands for wastewater treatment: design and operation. Bioresour Technol, 2015, 175: 594-601.

[54] WWAP (2017) The United Nations World Water Development Report 2017. Wastewater: The Untapped Resource, United Nations World Water Assessment Programme, Paris, UNESCO

[55] Yang Z, Zheng S, Chen J, Sun M. Purification of nitrate-rich agricultural runoff by a hydroponic system. Bioresour Technol, 2008, 99(17): 8049-8053.

[56] Yeboah SA, Allotey ANM, Bineye. Purification of industrial wastewater with vetiver grasses (Vetiveria zizanioides): the case of food and beverages wastewater In Ghana. Asian J Basic Appl Sci, 2015, 2(2): 2313-7797.

[57] Zhang DQ, Jinadasa K, Gersberg RM, Liu Y, Ng WJ, Tan SK. Application of constructed wetlands for wastewater treatment in developing countries–a review of recent developments (2000–2013). J Environ Manage, 2014, 141: 116-131.

[58] Zhao F, Yang W, Zeng Z, Li H, Yang X, He Z, Gu B, Rafiq MT, Peng H. Nutrient removal efficiency and biomass production of different bioenergy plants in hypereutrophic water. Biomass Bioenergy, 2012, 42: 212-218.

Funding

the United States Agency for International Development (USAID) under a USAID/HED funded grant in the Africa-US Higher Education Initiative(Grant HED 052-9740-ETH-11-01)