Magnetic ZIF-8/cellulose/Fe₃O₄ nanocomposite: preparation, characterization, and enzyme immobilization

Abstract

Background

The ZIF-8-coated magnetic regenerated cellulose-coated nanoparticles (ZIF-8@cellu@Fe₃O₄) were successfully prepared and characterized. The result showed that ZIF-8 was successfully composited on to the surface of the cellulose-coated Fe₃O₄ nanoparticles by co-precipitation method. Moreover, the glucose oxidase (GOx, from Aspergillus niger) was efficiently immobilized by the ZIF-8@Cellu@Fe₃O₄ nanocarriers with enhanced catalytic activities. The enzyme loading was 94.26 mg/g and the enzyme activity recovery was more than 124.2%. This efficiently immobilized enzyme exhibits promising applications in biotechnology, diagnosis, biosensing, and biomedical devices.

Conclusions

A new core–shell magnetic ZIF-8/cellulose nanocomposite (ZIF-8@Cellu@Fe₃O₄) was fabricated and structurally characterized. Glucose oxidase (GOx) was successfully immobilized by the biocompatible ZIF-8@Cellu@Fe₃O₄ with high protein loading (94.26 mg/g) and enhanced relative activity recovery (124.2%).

Keywords

Metal–organic frameworks / Glucose oxidase / Zeolitic imidazolate framework

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Funding

Fundamental Research Funds for the Chinese Universities(2015PT002)