Mineralization of a sulfonated textile dye Reactive Red 31 from simulated wastewater using pellets of Aspergillus bombycis

Abstract

Background

Reactive Red 31, applied extensively in the commercial textile industry, is a hazardous and persistent azo dye compound often present in dye manufacturing and textile industrial effluents. Aspergillus bombycis strain was isolated from dye contaminated zones of Gujarat Industrial Development Corporation, Vatva, Ahmedabad, India. The decolorization potential was monitored by the decrease in maximum absorption of the dye using UV–visible spectroscopy. Optimization of physicochemical conditions was carried out to achieve maximum decolorization of Reactive Red 31 by fungal pellets.

Results

Pellets of A. bombycis strain were found to decolorize this dye (20 mg/L) under aerobic conditions within 12 h. The activity of azoreductase, laccase, phenol oxidase and Manganese peroxidase in fungal culture after decolorization was about 8, 7.5, 19 and 23.7 fold more than before decolorization suggesting that these enzymes might be induced by the addition of Reactive Red 31 dye, and thus results in a higher decolorization. The lab-scale reactor was developed and mineralization of Reactive Red 31 dye by fungal pellets was studied at 6, 12 and 24 h of HRT (hydraulic retention time). At 12 h of HRT, decolorization potential, chemical oxygen demand (COD) and total organic carbon reduction (TOC) was 99.02, 94.19, and 83.97%, respectively, for 20 mg/L of dye concentration.

Conclusions

Dye decolorization potential of A. bombycis culture was influenced by several factors such as initial dye concentration, biomass concentration, pH, temperature, and required aerated conditions. Induction of azoreductase, laccase, phenol oxidase, and Mn-peroxidase enzymes was observed during dye decolorization phase. A. bombycis pellets showed potential in mineralization of dye in the aerobic reactor system. Isolated fungal strain A. bombycis showed better dye decolorization performance in short duration of time (12 h) as compared to other reported fungal cultures.

Keywords

Reactive Red 31 / Aspergillus bombycis / Pellets / Mineralization / GCMS

References

[1] Abd El-Rahim WM, Mostafa EM, Moawad H. High cell density cultivation of six fungal strains efficient in azo dye bioremediation. Biotechnol Rep, 2016, 12: 1-5.

[2] Abedin MAR. Decolorization and biodegradation of crystal violet and malachite green by Fusarium solani Martius Saccardo, a comparative study on biosorption of dyes by the dead fungal biomass. Am Euras J Bot, 2008, 12: 17-31.

[3] Ali N, Hameed A, Ahmed S. Physicochemical characterization and bioremediation perspective of textile effluent, dyes and metals by indigenous bacteria. J Hazard Mater, 2009, 164: 322-328.

[4] Ali N, Hameed A, Siddiqui MF, Ghumro PB, Ahmed S. Application of Aspergillus niger SA1 for the enhanced bioremoval of azo dyes in simulated textile effluent. Afr J Biotechnol, 2009, 8: 3839-3845.

[5] APHA, AWWA, WPCF (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington, DC

[6] Bergsten-Torralba LR, Nishikawa MM, Baptista DF, Magalhaes DP, Da Silva M. Decolorization of different textile dyes by Penicillium simplicissimum and toxicity evaluation after fungal treatment. Braz J Microbiol, 2009, 40: 808-817.

[7] Borchert M, Libra JA. Decolorization of reactive dyes by the white rot fungus Trameters versicolor in sequencing batch reactors. Biotechnol Bioeng, 2001, 75: 313-321.

[8] Chakraborty S, Basak B, Dutta S, Bhunia B, Dey A. Decolorization and biodegradation of Congo Red dye by a novel white rot fungus Alternaria alternata CMERI F6. Bioresour Technol, 2013, 147: 662-666.

[9] Chen SH, Ting ASY. Biodecolorization and biodegradation potential of recalcitrant triphenylmethane dyes by Coriolopsis sp. isolated from compost. J Environ Manag, 2015, 150: 274-280.

[10] Espinosa-Ortiz EJ, Rene ER, Pakshirajan K, Van Hullebusch ED, Lens PNL. Fungal pelleted reactors in wastewater treatment: applications and perspectives. Chem Eng J, 2016, 283: 553-571.

[11] Fewson CA. Biodegradation of xenobiotic and other persistent compounds: the causes of recalcitrance. Trends Biotechnol, 1988, 6: 148-153.

[12] Fu Y, Viraraghavan T. Removal of acid blue 29 from an aqueous solution by Aspergillus niger. Am Assoc Text Chem Color Rev, 2001, 1(1): 36-40.

[13] Gomi N, Yoshida S, Matsumoto K, Okudomi M, Konno H, Hisabori T, Sugano Y. Degradation of the synthetic dye amaranth by the fungus Bjerkandera adusta Dec 1: inference of the degradation pathway from an analysis of decolorized products. Biodegradation, 2011, 22: 1239-1245.

[14] Hadibarata T, Yusoff A, Aris A, Salmiati S, Hidayat T, Kristanti R. Decolorization of azo triphenylmethane and anthraquinone dyes by laccase of a newly isolated Armillaria sp. F022. Water Air Soil Pollut, 2012, 223: 1045-1054.

[15] Hai FI, Yamamoto K, Nakajima F, Fukushi K, Nghiem LD, Price WE, Jin B. Degradation of azo dye acid orange 7 in a membrane bioreactor by pellets and attached growth of Coriolus versicolour. Bioresour Technol, 2013, 141: 29-34.

[16] Jadhav JP, Govindwar SP. Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463. Yeast, 2006, 23: 315-323.

[17] Jin XC, Liu GQ, Xu ZH, Tao WY. Decolorization of a dye industry effluent by Aspergillus fumigatus XC6. Appl Microbiol Biotechnol, 2007, 74: 239-243.

[18] Kalyani DC, Telke AA, Govindwar SP, Jadhav JP. Biodegradation and detoxification of reactive textile dye by isolated Pseudomonas sp. SUK1. Water Environ Res, 2009, 81: 298-307.

[19] Karthikeyan K, Nanthakumar K, Shanthi K, Lakshmanaperumalsamy P. Response surface methodology for optimization of culture conditions for dye decolorization by a fungus Aspergillus niger HM11 isolated from dye affected soil. Iran J Microbiol, 2010, 2: 213-222.

[20] Kaushik R, Grochowska KM, Butnaru I, Kreutz MR. Protein trafficking from synapse to nucleus in control of activity-dependent gene expression. Neuroscience, 2014, 280: 340-350.

[21] Khan R, Bhawana P, Fulekar MH. Microbial decolorization and degradation of synthetic dyes: a review. Rev Environ Sci Biotechnol, 2013, 12: 75-97.

[22] Khan R, Khan Z, Bhatt N, Devecha J, Madamwar D. Azo dye decolorization under microaerophilic conditions by a bacterial mixture isolated from anthropogenic dye-contaminated soil. Bioremediat J, 2014, 18(2): 147-157.

[23] Lang W, Sirisansaneeyakul S, Ngiwsara L, Mendes S, Martins LO, Okuyama M, Kimura A. Characterization of a new oxygen-insensitive azo reductase from Brevibacillus laterosporus TISTR1911: toward dye decolorization using a packed-bed metal affinity reactor. Bioresour Technol, 2013, 150: 298.

[24] Manai I, Miladi B, Mselmi AE, Smaali I, Hassen A, Hamd M, Bouallagui H. Industrial textile effluent decolourization in stirred and static batch cultures of a new fungal strain Chaetomium globosum IMA1 KJ472923. J Environ Manag, 2016, 170: 8-14.

[25] Munari FM, Gaio TA, Calloni R, Dillon AJP. Decolorization of textile dyes by enzymatic extract and submerged cultures of Pleurotus sajor-caju. World J Microbiol Biotechnol, 2008, 24: 1383-1392.

[26] Nilsson I, Moller A, Mattiasson B, Rubindamayugi MST, Welander U. Decolorization of synthetic and real textile wastewater by the use of white-rot fungi. Enzyme Microb Technol, 2006, 38: 94-100.

[27] Pandey A, Singh P, Iyengar L. Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegrad, 2007, 59: 73-84.

[28] Parshetti GK, Parshetti SG, Telke AA, Kalyani DC, Doong RA, Govindwar SP. Biodegradation of crystal violet by Agrobacterium radiobacter. J Environ Sci, 2011, 23(8): 1384-1393.

[29] Pilatin S, Kunduhoglu B. Decolorization of textile dyes by newly isolated Trametes versicolor strain. Life Sci Biotechnol, 2011, 1: 125-135.

[30] Radha KV, Regupathi I, Arunagiri A, Murugesan T. Decolorization studies of synthetic dyes using Phanerochaete chrysosporium and their kinetics. Process Biochem, 2005, 40: 3337-3345.

[31] Raghukumar C, Chandramohan D, Michel F Jr, Reddy CA. Degradation of lignin and decolourisation of paper mill bleach plant effluent (BPE) by marine fungi. Biotech Lett, 1996, 24: 1757-1761.

[32] Salar RK, Aneja KR. Thermophilic fungi: taxonomy and biogeography. J Agric Technol, 2007, 3(1): 77-107.

[33] Saratale GD, Kalme SD, Govindwar SP. Decolorization of textile dyes by Aspergillus ochraceus (NCIM-1146). Indian J Biotechnol, 2006, 5: 407-410.

[34] Saratale GD, Saratale RG, Chang JS, Govindwar SP. Fixed-bed decolorization of Reactive Blue 172 by Proteus vulgaris NCIM-2027 immobilized on Luffa cylindrica sponge. Int Biodeterior Biodegrad, 2011, 65: 494-503.

[35] Saratale RG, Gandhi SS, Purankar MV, Kurade MB, Govindwar SP, Oh SE, Saratale GD. Decolorization and detoxification of sulfonated azo dye C.I. Remazol Red and textile effluent by isolated Lysinibacillus sp. RGS J Biosci Bioeng, 2013, 115: 658-667.

[36] Sen K, Pakshirajan K, Santra SB. Modelling the biomass growth and enzyme secretion by the white rot fungus Phanerochaete chrysosporium in presence of a toxic pollutant. J Environ Prot, 2012, 3: 114-119.

[37] Shedbalkar U, Dhanve R, Jadhav J. Biodegradation of triphenylmethane dye cotton blue by Penicillium ochrochloron MTCC 517. J Hazard Mater, 2008, 157: 472-479.

[38] Singh H. Mycoremediation: fungal bioremediation, 2006, Hoboken: Wiley

[39] Singh S, Pakshirajan K, Daverey A. Enhanced decolourization of Direct Red-80 dye by the white rot fungus Phanerochaete chrysosporium employing sequential design of experiments. Biodegradation, 2010, 21: 501-511.

[40] Sinha A, Osborne WJ. Biodegradation of reactive green dye (RGD) by indigenous fungal strain VITAF-1. Int Biodeterior Biodegrad, 2016, 114: 176-183.

[41] Solís M, Solís A, Pérez H, Manjarrez N, Flores M. Microbial decoloration of azo dyes: a review. Process Biochem, 2012, 47: 1723-1748.

[42] Srinivasan A, Viraraghavan T. Decolorization of dye wastewaters by biosorbents: a review. J Environ Manag, 2010, 91: 1915-1929.

[43] Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011, 28: 2731-2739.

[44] Tang W, Jia R, Zhang D. Decolorization and degradation of synthetic dyes by Schizophyllum sp. F17 in a novel system. Desalination, 2011, 265: 22-27.

[45] Xin B, Chen G, Zheng W. Bioaccumulation of Cu-complex reactive dye by growing pellets of Penicillium oxalicum and its mechanism. Water Res, 2010, 44: 3565-3572.

[46] Zhang X, Flurkey WH. Phenoloxidases in Portabella mushrooms. J Food Sci, 1997, 62: 97-100.

[47] Zhao LH, Zhou JT, Zheng CL, Yang YS, Sun HJ, Zhang XH. Decolorization of cotton pulp black liquor by Pleurotus ostreatus in a bubble-column reactor. Bull Environ Contam Toxicol, 2008, 80: 44-48.