Industrial cellulase performance in the simultaneous saccharification and co-fermentation (SSCF) of corn stover for high-titer ethanol production

Abstract

Background

Cellulase enzymes contribute to the largest portion of operation cost on production of cellulosic ethanol. The industrial cellulases available on the industrial enzyme market from different makers and sources vary significantly in hydrolysis and ethanol, and finally lead to the changes of enzyme cost. Therefore, the selection of the proper industrial cellulase enzymes for commercial-scale production of cellulosic ethanol is crucially important in terms of high performance and cost reduction.

Results

In this study, three major cellulase enzyme products available on the Chinese industrial enzyme market were selected and evaluated as the biocatalysts for the biorefining process of lignocellulose biomass into high-titer ethanol. The cellulase enzymes included Cellic CTec 2.0 from Novozymes (Beijing), and LLC 4 from Vland (Qingdao), as well as # 7 from an industrial enzyme maker. The detailed assays on the filter paper activity, the cellobiase activity, and the total protein contents of the enzymes were conducted according to the standard protocols. When the cellulase enzymes were applied to the practical hydrolysis and ethanol-fermentation operation under the conditions of high solids loading and low range of cellulase dosage, the hydrolysis yield shows the significant difference, and the difference was narrowed in the final ethanol yield.

Conclusions

The commercially available cellulase enzymes showed different performances in the activities, the cellulose hydrolysis yield, and the ethanol fermentation yields based on the protein dosage per gram of cellulose of corn stover. In general, the industrial cellulase products give satisfactory performance and can be applied for the practical cellulosic ethanol production on commercial scale.

Keywords

Industrial cellulase enzyme / Activity / Ethanol / Hydrolysis / Lignocellulose

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Funding

High-Tech Program of China(2014AA021901)