Palm oil mill effluent as a low-cost substrate for bioflocculant production by Bacillus marisflavi NA8

Abstract

Background

Palm oil mill effluent (POME) generated from the palm oil milling process contains high organic matter from which the molecule of interest for bioconversion such as fermentable sugar can be derived. This study examined the enzymatic hydrolysis of complex celluloses and polysaccharides present in POME with the aim to release the simple sugars as low-cost fermentable feedstock convertible into bioflocculant.

Results

The maximum concentration of fermentable sugar produced after 24 h of hydrolysis was 30.5 g/L. The POME hydrolysate served as a carbon source for Bacillus marisflavi NA8 to produce bioflocculant. The optimum conditions obtained were an initial pH of 7.0 and a temperature of 37 °C with an inoculum size of 5 % (v/v), yielding 6.4 g/L or 32 kg/t bioflocculant from POME.

Conclusion

This finding indicated that POME could be utilized as a low-cost substrate to improve the feasibility of commercial production of bioflocculant.

Keywords

Palm oil mill effluent / Enzymatic hydrolysis / Sugar / Bioflocculant / Bacillus marisflavi NA8

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