Aspergillus oryzae lipase-catalyzed synthesis of glucose laurate with excellent productivity

Abstract

Background

As nonionic surfactants derived from naturally renewable resources, sugar fatty acid esters (SFAEs) have been widely utilized in food, cosmetic, and pharmaceutical industries.

Results

In this study, six enzymes were screened as catalyst for synthesis of glucose laurate. Aspergillus oryzae lipase (AOL) and Aspergillus niger lipase (ANL) yielded conversions comparable to the results obtained by commercial enzymes such as Novozyme 435 and Lipozyme TLIM. The productivity obtained by AOL catalysis in anhydrous 2–methyl–2–butanol (2M2B) (38.7 mmol/L/h and 461.0 μmol/h/g) was much higher than the other literature results. Factors affecting the synthetic reaction were investigated, including water content, enzyme amount, substrate concentrations and reaction temperature. The process was greatly improved by applying the Box-Behnken design of response surface methodology (RSM). Solubilities of glucose in 14 different organic solvents were determined, which were found to be closely associated with the polarity of the solvents.

Conclusions

Aspergillus oryzae lipase is a promising enzyme capable of efficiently catalyzing the synthesis of sugar fatty acid esters with excellent productivity.

Keywords

Aspergillus oryzae lipase (AOL) / Aspergillus niger lipase (ANL) / Sugar fatty acid ester (SFAE) / Glucose laurate / Organic media / Response surface methodology (RSM) / Box-Behnken design (BBD)

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Funding

National Natural Science Foundation of China (CN)(21276159)