Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction

Abstract

Background

Biopalladium (bioPd(0)) nanoparticles on Klebsiella Pneumoniae ECU-15 were synthesized mainly on the microorganism's surface. Data suggest that the resistance of mass transfer around the cell surface region plays a critical role in bioPd(0) synthesis process. However, the mechanisms for its role remains elusive.

Results

The experimental results indicated that 1) diffusion resistance existed around the microorganism's cell in reaction vessel and 2) fluid shear stress affected the mass transfer rates differently according to its strength and thus had varying effects on the bioPd(0) synthesis. More than 97.9 ± 1.5% Chromium(VI)(Cr(VI)) (384 μM) was reduced to Cr(III) within 20 min with 5% Pd/bioPd(0) as catalyst, which was generated by the K. Pneumoniae ECU-15, and the catalytic performance of Pd/bioPd(0) was stable over 6 months. The optimal condition of bioreduction of Pd(II) to Pd(0) was determined at the Kolmogorov eddy length of 7.33 ± 0.5 μm and lasted for 1 h in the extended reduction process after the usual adsorption and reduction process.

Conclusions

It is concluded that a high bioPd(0) catalytic activity can be achieved by controlling the fluid shear stress intensity in an extended reduction process in the bioreactor.

Keywords

Biopalladium / Mass transfer resistance / Fluid shear stress / K. pneumoniae

References

[1] Yong P, Rowson NA, Farr JPG, Harris IR, Macaskie LE Bioreduction and biocrystallization of palladium by Desulfovibrio desulfuricans NCIMB 8307. Biotechnol Bioeng, 2002, 80: 369-379.

[2] Hennebel T, De Gusseme B, Boon N, Verstraete W. Biogenic metals in advanced water treatment. Trends Biotechnol, 2009, 27: 90-98.

[3] Rotaru AE, Jiang W, Finster K, Skrydstrup T, Meyer RL. Non‐enzymatic palladium recovery on microbial and synthetic surfaces. Biotechnol Bioeng, 2012, 109: 1889-1897.

[4] Karthikeya S, Beveridge TJ. Pseudomonas aeruginosa biofilms react with and precipitate toxic soluble gold. Environ Microbiol, 2002, 4: 667-675.

[5] Humphries AC, Nott KP, Hall LD, Macaskie LE. Reduction of Cr(VI) by immobilized cells of Desulfovibrio vulgaris NCIMB 8303 and Microbacterium sp. NCIMB 13776. Environ Microbiol, 2005, 90: 589-596.

[6] Lloyd JR, Yong P, Macaskie LE. Enzymatic recovery of elemental palladium by using sulfate-reducing bacteria. Appl Environ Microbiol, 1998, 64: 4607-4609.

[7] Creamer NJ, Mikheenko IP, Yong P, Deplanche K, Sanyahumbi D, Wood J, Pollmann K, Merroun M, Selenska-Pobell S, Macaskie L. Novel supported Pd hydrogenation bionanocatalyst for hybrid homogeneous/heterogeneous catalysis. Catal Today, 2007, 128: 80-87.

[8] De Windt W, Aelterman P, Verstraete W. Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls. Environ Microbiol, 2005, 7: 314-325.

[9] Deplanche K, Caldelari I, Mikheenko IP, Sargent F, Macaskie LE. Involvement of hydrogenases in the formation of highly catalytic Pd (0) nanoparticles by bioreduction of Pd (II) using Escherichia coli mutant strains. Microbiol, 2010, 156: 2630-2640.

[10] Martins M, Assuncao A, Martins H, Matos AP, Costa MC. Palladium recovery as nanoparticles by an anaerobic bacterial community. J Chem Technol Biotechnol, 2013, 88: 2039-2045.

[11] Chidambaram D, Hennebel T, Taghavi S, Mast J, Boon N, Verstraete W, van der Lelie D, Fitts JP. Concomitant microbial generation of palladium nanoparticles and hydrogen to immobilize chromate. Environ Sci Technol, 2010, 44: 7635-7640.

[12] Niu K, Zhang X, Tan WS, Zhu ML. Characteristics of fermentative hydrogen production with Klebsiella pneumoniae ECU-15 isolated from anaerobic sewage sludge. Int J Hydrogen Energy, 2010, 35: 71-80.

[13] Hennebel T, Van Nevel S, Verschuere S, De Corte S, De Gusseme B, Cuvelier C, Fitts JP, Van der Lelie D, Boon N, Verstraete W. Palladium nanoparticles produced by fermentatively cultivated bacteria as catalyst for diatrizoate removal with biogenic hydrogen. Appl Microbiol Biotechnol, 2011, 91: 1435-1445.

[14] Deplanche K, Merroun ML, Casadesus M, Tran DT, Mikheenko IP, Bennett JA, Zhu J, Jones IP, Attard GA, Swlenska-Pobell S, Macaskie LE. Microbial synthesis of core/shell gold/palladium nanoparticles for applications in green chemistry. J R Soc Interface, 2012, 9: 1705-1712.

[15] Han GZ, Chen MD. Microwave peak absorption frequency of liquid. S Sci China Ser G-Phys Mech Astron, 2008, 51: 1254-1263.

[16] Tzima E, Irani-Tehrani M, Kiosses WB, Dejana E, Schultz DA, Engelhardt B, Cao G, DeLisser H, Schwartz MA. A mechanosensory complex that mediates the endothelial cell response to fluid shear stress. Nature, 2005, 437: 426-431.

[17] Deplanche K, Mikheenko I, Bennett J, Merroun M, Mounzer H, Wood J, Macaskie L. Selective oxidation of benzyl-alcohol over biomass-supported Au/Pd bioinorganic catalysts. Top Catal, 2011, 54: 1110-1114.

[18] Bunge M, Sobjerg LS, Rotaru AE, Gauthier D, Lindhardt AT, Hause G, Finster K, Kingshott P, Skrydstrup T, Meyer RL. Formation of palladium (0) nanoparticles at microbial surfaces. Biotechnol Bioeng, 2010, 107: 206-215.

[19] Wang RF, Wang H, Feng HQ, Ji S. Palladium decorated nickel nanoparticles supported on carbon for formic acid oxidation. Int J Electrochem Sci, 2013, 8: 6068-6076.

[20] Tobin JM, White C, Gadd GM. Metal accumulation by fungi: applications in environmental biotechnology. J Ind Microbiol, 1994, 13: 126-130.

[21] Gumbart JC, Beeby M, Jensen GJ, Roux B. Escherichia coli peptidoglycan structure and mechanics as predicted by atomic-scale simulations. Plos Comput Biol, 2014, 10: 1003475.

[22] Nies DH. Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiol Rev, 2003, 27: 313-339.

[23] Hodgson L, Tarbell JM. Solute transport to the endothelial intercellular cleft: the effect of wall shear stress. Ann Biomedl Eng, 2002, 30: 936-945.

[24] Silva-Santisteban BOY, Filho FM. Agitation, aeration and shear stress as key factors in inulinase production by Kluyveromyces marxianus. Enzyme Microb Technol, 2005, 36: 717-724.

[25] Lantz J, Gardhagen R, Karlsson M. Quantifying turbulent wall shear stress in a subject specific human aorta using large eddy simulation. Med Eng Phys, 2012, 34: 1139-1148.

[26] Allen JJ, Shockling MA, Kunkel GJ, Smits AJ. Turbulent flow in smooth and rough pipes. Phil Trans R Soc A, 2007, 365: 699-714.

[27] Onishi R, Matsuda K, Takahashi K, Kurose R, Komori S. Retrieval of collision kernels from the change of droplet size distributions with linear inversion. Phys Scripta, 2008, 2008: 014050.

[28] Klaewkla R, Arend M, Hoelderich WF. A review of mass transfer controlling the reaction rate in heterogeneous catalytic systems. de Mass Transfer-Advanced Aspects, 2011, Germany: InTech, 668-684.

[29] Evans JR, Davids WG, MacRae JD, Amirbahman A. Kinetics of cadmium uptake by chitosan-based crab shells. Water Res, 2002, 36: 3219-3226.

[30] Ghadge RS, Patwardhan AW, Joshi JB. Combined effect of hydrodynamic and interfacial flow parameters on lysozyme deactivation in a stirred tank bioreactor. Biotechnol Prog, 2006, 22: 660-672.

[31] Cherry RS, Papoutsakis ET. Hydrodynamic effects on cells in agitated tissue culture reactors. Bioproc Eng, 1986, 1: 29-41.