Use of Restricted Nursing and a Bovine Appeasing Substance on the Welfare, Growth Performance, and Response to Vaccination of Beef Calves at Weaning

Denise E. Mussalafo , Marcelo Vedovatto , Aylpy R. D. Santos , Dalton M. Oliveira , Fabiana A. M. Sterza , Mariana Santos , Helena B. C. R. Batista , Jaíne G. Garcia , Andréa R. D. L. Souza

Animal Research and One Health ›› 2026, Vol. 4 ›› Issue (3) : 314 -321.

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Animal Research and One Health ›› 2026, Vol. 4 ›› Issue (3) :314 -321. DOI: 10.1002/aro2.70031
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Use of Restricted Nursing and a Bovine Appeasing Substance on the Welfare, Growth Performance, and Response to Vaccination of Beef Calves at Weaning
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Abstract

This study evaluated the effects of restricted nursing (RN) and the administration of a bovine appeasing substance (BAS) given 8 days before (d-8) and on the day of weaning (d0) as a strategy to mitigate stress and optimize the post-weaning growth performance of beef calves, with emphasis on behavior and response to vaccination. Forty male Nelore calves were randomly assigned to four treatments with ten replicates: control (CON)—topical administration of 5 mL of saline solution on d0; BAS-8—topical administration of 5 mL of BAS on d-8; BAS0—topical administration of 5 mL of BAS on d0; and RN—calves allowed to nurse only one hour/day from d-8 to d0. Calves in the CON treatment group showed lower average daily gain compared to the other treatments (p < 0.05). There was a trend (p = 0.09) for BAS-8 and BAS0 to result in lower temperament scores when calves entered the restraint chute. BAS0 calves had greater (p = 0.05) serum titer concentrations against rabies compared to the other treatments. The RN and BAS administration eight days before and on the day of weaning was effective in improving growth performance in beef calves.

Keywords

average daily gain / body weight / nelore / stress / temperament

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Denise E. Mussalafo, Marcelo Vedovatto, Aylpy R. D. Santos, Dalton M. Oliveira, Fabiana A. M. Sterza, Mariana Santos, Helena B. C. R. Batista, Jaíne G. Garcia, Andréa R. D. L. Souza. Use of Restricted Nursing and a Bovine Appeasing Substance on the Welfare, Growth Performance, and Response to Vaccination of Beef Calves at Weaning. Animal Research and One Health, 2026, 4 (3) : 314-321 DOI:10.1002/aro2.70031

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2025 The Author(s). Animal Research and One Health published by John Wiley & Sons Australia, Ltd on behalf of Institute of Animal Science, Chinese Academy of Agricultural Sciences.

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