Establishment of Interspecies Somatic Cell Nuclear Transfer and Transgene-Free Inducible Pluripotent Stem Cells for Versatile Conservation of the Germplasm Resource of Wild Boar

Abstract

Cloning via somatic cell nuclear transfer and establishing inducible pluripotent stem cells is critical for the conservation of the cherished germplasm resource. The wild boar population would be altered due to human activities and climate change. Recently, we have successfully generated cloned wild boars via interspecies somatic cell nuclear transfer using wild boar fibroblast cells as donor cells, domestic porcine oocytes as recipients, and Bama pigs as surrogate sows. We have also harvested wild boar inducible pluripotent stem cells free of selection genes utilizing the piggyBac transposon system. This study sheds light on preserving the genetic resource of wild boar and provides a reference for protecting other wildlife.

Keywords

germplasm resource / pig / reprogramming / somatic cell nuclear transfer / stem cells

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2025 The Author(s). Animal Research and One Health published by John Wiley & Sons Australia, Ltd on behalf of Institute of Animal Science, Chinese Academy of Agricultural Sciences.