miR-429 inhibits palmitic acid-induced apoptosis of porcine subcutaneous preadipocytes by targeting Sox5

Abstract

MicroRNAs (miRNAs) can regulate several physiological processes of cells after transcription, such as cell proliferation, differentiation, and apoptosis. In this study, we found that 500 µM of palmitic acid (PA) could significantly induce the apoptosis of porcine subcutaneous preadipocytes (p < 0.05). The overexpression of miR-429 decreased the apoptotic rate of porcine preadipocytes and inhibited the expression of the proapoptotic gene P53 (p < 0.05). In addition, miR-429 can specifically bind to the 3’ untranslated region of Sox5, and the upregulation of miR-429 downregulated Sox5 expression. However, Sox5 overexpression promoted the apoptosis of porcine preadipocytes (p < 0.01); the co-transfection of miR-429 and pcDNA3.1-Sox5 into preadipocytes could reverse the inhibition of PA-induced apoptosis by miR-429. In conclusion, the present results provide a theoretical basis for elucidating the molecular mechanisms by which miR-429 and Sox5 regulate the apoptosis of porcine subcutaneous preadipocytes.

Keywords

apoptosis / miR-429 / palmitic acid (PA) / porcine subcutaneous preadipocytes / Sox5

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2024 The Authors. Animal Research and One Health published by John Wiley & Sons Australia, Ltd on behalf of Institute of Animal Science, Chinese Academy of Agricultural Sciences.