Serotype-dependent cytopathic effects and replication dynamics of dengue virus serotypes 1 and 2 in Chang liver cells

Conflict of interest statement

The authors declare that they have no conflict of interest.

Funding

This work was funded by the FUNDAMENTAL RESEARCH GRANT SCHEME, Malaysia Ministry of Higher Education (MoHE), grant number: FRGS/1/2021/STG03/UKM/02/2.

Authors’ contributions

SASR, AIY, NAHMA and WBY jointly developed the study concepts and design, performed literature search, SASR, AIY and NAHMA completed experimental studies and data analysis, and SASR, AIY and NAHMA prepared the manuscript. RSAS carried out manuscript review; AIY undertook manuscript editing; WBY defined intellectual content, revised the manuscript and acted as the guarantor. All authors read and approved the final version of the manuscript.

Publisher’s note

The Publisher of the Journal remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Edited by Pan Y, Lei Y, Zhang Q

References

[1] Johari NA, Voon K, Toh SY, Sulaiman LH, Yap IKS, Lim PKC. Sylvatic dengue virus type 4 in Aedes aegypti and Aedes albopictus mosquitoes in an urban setting in Peninsular Malaysia. PLoS Negl Trop Dis 2019; 13(11): e0007889.

[2] Fibriansah G, Ng TS, Kostyuchenko VA, Shi J, Lok SM. Cryo-EM structure of dengue virus serotype 1 strain WestPac 74 in complex with human antibody 1C19 Fab at 37 deg C (Class 1 particle). Worldwide Protein Data Bank 2021. doi: 10.2210/pdb7dwt/pdb.

[3] Holmes EC, Twiddy SS. The origin, emergence and evolutionary genetics of dengue virus. Infect Genet Evol 2003; 3(1): 19-28.

[4] Rani K, Tripathi S, Dahiya D, Kundu S, Raj VS, Sahal D, et al. Dengue virus: A global concern and advances in treatment strategies. Microbe 2025; 9(1): 100591.

[5] Gallichotte EN, Baric TJ, Nivarthi U, Delacruz MJ, Graham R, Widman DG, et al. Genetic variation between dengue virus type 4 strains impacts human antibody binding and neutralization. Cell Rep 2018; 25(5): 1214-1224.

[6] Yung CF, Lee KS, Thein TL, Tan LK, Gan VC, Wong JGX, et al. Dengue serotype-specific differences in clinical manifestation, laboratory parameters and risk of severe disease in adults, Singapore. Am J Trop Med Hyg 2015; 92(5): 999.

[7] Póvoa TF, Alves AMB, Oliveira CAB, Nuovo GJ, Chagas VLA, Paes MV. The pathology of severe dengue in multiple organs of human fatal cases: Histopathology, ultrastructure and virus replication. PLoS One 2014; 9(4): e83386.

[8] Gutiérrez-Barbosa H, Castañeda NY, Castellanos JE. Differential replicative fitness of the four dengue virus serotypes circulating in Colombia in human liver Huh7 cells. Brazilian J Infect Dis 2020; 24(1): 13-24.