Chemical composition and wound healing potential of Lindernia ruellioides (Colsmann) Pennell from Mizoram, India

Abstract

Objective: To explore the wound healing potential and chemical components of Lindernia ruellioides from Mizoram, India.

Methods: Plant extraction was conducted by cold maceration using chloroform, ethanol, and distilled water as solvents. To guarantee the safety of the plant extract, an acute dermal toxicity test was conducted before the experiment. Antioxidant assays were performed. Excision and incision wound models were used to assess the wound healing activities, including wound closure rate, epithelialization period, and tensile strength. A histopathological examination was carried out.

Results: Lindernia ruellioides contained bioactive compounds, such as mycolactone F, 1-O-octadec-9-enyl glycerol, reserpine, tetracosanoic acid, 2-O-caffeoylglucarate and several others which are found to possess various pharmacological activities. Acute dermal toxicity evaluation showed that the doses were deemed safe. Ethanol extract of Lindernia ruellioides (LREE) treatment significantly elevated glutathione, glutathione-S-transferase, and superoxide dismutase, and decreased malondialdehyde in a dose- dependent manner. It showed increased wound contraction rate, shorter epithelialization time, and elevated wound breaking strength in the LREE-treated group when compared with the control. In addition, the histological examination showed enhanced neovascularization, fibroblasts, and collagen in LREE-treated animals.

Conclusions: Lindenia ruellioides exhibits remarkable effects on wound healing. The study validates the traditional use of this plant in Mizoram region as a wound-healing agent.

Keywords

Lindenia ruellioides / Ethanolic extract / LC-MS / Antioxidant / Wound healing

Conflict of interest statement

The authors declare that there is no conflict of interest.

Funding

The authors received no extramural funding for the study.

Data availability statement

The data supporting the findings of this study are available from the corresponding author upon request.

Authors’ contributions

CL contributed to designing the experiment, data curation, investigation, writing the manuscript, and reviewing and editing the manuscript. RKL assisted with the experiment. EL was involved in supervision, and conceptualization as well as provided lab equipment and chemicals. The final manuscript was read and approved by all of the authors.

Publisher’s note

The Publisher of the Journal remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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