Analgesic and anti-inflammatory activity of Cotinus coggygria Scop. extracts in vivo

Abstract

Objective: To assess the analgesic and anti-inflammatory effects of standardized extract of Cotinus coggygria ( C. coggygria) in different animal models. Methods: C. coggygria extracts (25, 50, and 100 mg/kg) were administered to rats and mice (n=6) during hot plate, tail-flick, acetic acid-induced writhing, and formalin tests to determine its analgesic efficacy. The anti-inflammatory activity of C. coggygria extracts was evaluated by histamine and carrageenan-induced paw edema, cotton pellet-induced granuloma, and acetic acid-induced peritoneal capillary dye leakage tests. Results: C. coggygria extracts (50 and 100 mg/kg) significantly alleviated thermal and chemical-induced pain in rodents (P<0.05). It also demonstrated notable anti-inflammatory properties by mitigating histamine and carrageenan-induced paw edema, granuloma deposits, and vascular permeability (P<0.05). Moreover, C. coggygria extracts remarkably reduced TNF-α, IL-1β, IL-6, COX-2, and oxidative stress in rat paws (P<0.05). Carrageenan-induced histological aberrations in hind paw tissues were effectively (P<0.05) mitigated by treatment with C. coggygria extracts. Conclusions: C. coggygria Scop. extracts show analgesic and anti-inflammatory effects via inhibition of COX-2 and inflammatory and oxidative mediators.

Keywords

Antioxidant / Carrageenan / Cotinus coggygria Scop. / Anti-inflammatory / Analgesic / Pain / TNF-α / Fustin

Acknowledgments

We thanks Dr. Amit D. Kandhare for his guidance and assistance in this study.

Conflict of interest statement

The authors have no conflicts of interest.

Funding

The authors received no extramural funding for the study.

Data availability statement

The data supporting the findings of this study are available from the corresponding authors upon request.

Authors’ contributions

KL conceptualized the work and performed data analysis and interpretation. CYY collected the data. ZHY performed the data analysis and interpretation, and drafted the article. All the authors critically revised the article, and approved the final version of the manuscript.

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