Biochanin-A attenuates high-fat diet and streptozotocin-induced hyperlipidemia and oxidative stress in rats by improving antioxidant status and lipid metabolic markers

Abstract

Objective: To determine how biochanin-A (BCA) affects high-fat diet and streptozotocin-induced pathological changes in lipid metabolism and antioxidant status in diabetic rats. Methods: Diabetic rats were orally administered BCA (10 mg/kg body weight) for 30 days to investigate its effects on lipid profiles and oxidative stress markers in the liver and kidney. In addition, the mRNA expression of antioxidant and lipid metabolism enzymes in the liver was examined. Results: BCA attenuated hyperlipidemia by regulating mRNA expressions of HMG-CoA reductase, fatty acid synthase, carnitine palmitoyl transferase, and acetyl-CoA carboxylase. Additionally, BCA reduced high-fat diet and streptozotocin-induced oxidative stress by suppressing lipid peroxidation, improving superoxide dismutase, catalase, and glutathione peroxidase levels, and upregulating mRNA expressions of these enzymes. Conclusions: BCA may be a promising nutraceutical for the treatment of dyslipidemia and oxidative stress associated with diabetes.

Keywords

Antioxidant / Biochanin-A / Nutraceutical / Obesity / Hyperlipidemia / Type 2 diabetes mellitus / Oxidative stress

Acknowledgments

The authors would like to express their sincere thanks to the management of K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Tamilnadu, India for providing the necessary facilities to conduct this work and their constant support. Further, we extend our sincere thanks to the management of Nandha College of Pharmacy, Erode, Tamilnadu, and Arulmigu Kalasalingam College of Pharmacy, Krishnankoil, Tamilnadu for providing an animal house facility to carry out animal experiments.

Significance

Biochanin-A is an O-methylated isoflavonoid found in various plants and has biological and pharmacological effects. However, there has been no solid scientific evidence on the effect of biochanin-A against high-fat diet and streptozotocin-induced pathological changes in lipid metabolism and antioxidant status. The results of the current study show that biochanin-A alleviated type 2 diabetes mellitus by attenuating hyperlipidemia and oxidative stress via regulating lipid metabolism and antioxidant genes. Therefore, this compound may be further investigated for use as a promising nutraceutical for the treatment of dyslipidemia and oxidative stress associated with diabetes.

Conflict of interest statement

The authors declare that there is no conflict of interest.

Funding

The authors received no extramural funding for the study.

Data availability statement

The data supporting the findings of this study are available from the corresponding authors upon request.

Authors’ contributions

PPS and VVSU wrote the manuscript; PPS, VVSU, PC, SS and PT performed experiments and collected the data; PP, SV, MS and MSB analyzed the data; PPS, VVSU and GS designed the study; GS reviewed the manuscript. All authors read and approved the final manuscript.

Publisher’s note

The Publisher of the Journal remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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