Inhibitory effect of Cyrtomium falcatum on melanogenesis in α-MSH-stimulated B16F10 murine melanoma cells

Abstract

Objective: To explore the anti-melanogenic potential of Cyrtomium falcatum. Methods: The effects of Cyrtomium falcatum crude extract and its solvent fractions on tyrosinase activity, melanin content, and the expressions of melanogenesis-related genes and proteins were analyzed in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 cells. Results: α-MSH treatment significantly increased tyrosinase activity, and extracellular and intracellular melanin content, as well as the expression levels of tyrosinase, microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)-1, and TRP-2 in B16F10 cells. Treatment with Cyrtomium falcatum crude extract and its solvent fractions reduced tyrosinase activity and extracellular and intracellular melanin content and downregulated the expression levels of tyrosinase, MITF, TRP-1, and TRP-2 in a dose-dependent manner. Conclusions: Cyrtomium falcatum has potential anti-melanogenesis effects and can be used as a potential source material in cosmeceutical industry for the research and development of novel lead molecules with whitening properties.

Keywords

Cyrtomium falcatum / Melanogenesis / α-MSH / B16F10 melanoma cells

Conflict of interest statement

The authors declare that they have no conflict of interest.

Funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2023R1A2C1006268 and RS-2023-00212560).

Authors’ contributions

XRZ and CSK conceived the idea and designed the study. XRZ, JHO, HL, HEK and MJ performed the experiments, acquired the data and validated the results. XRZ and FK drafted the original manuscript and XRZ, FK and YS contributed to the revisions. JHO and YS performed the extraction and fractionation. JHO, FK, YS and CSK performed the statistical analysis, data curation, and validation and visualization of the results. YS and CSK supervised the project.

Publisher’s note

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