Green synthesis, characterization, acaricidal, larvacidal, and repellent activities of copper nanoparticles of Astragalus sinicus against Hyalomma anatolicum

Abstract

Objective: To green synthesize and characterize copper nanoparticles (CuNPs) using Astragalus sinicus, as well as evaluate the acaricidal, larvacidal, and repellent activities of CuNPs against Hyalomma anatolicum ( H. anatolicum), one of the most prevalent ticks infesting cattle in Saudi Arabia. Methods: CuNPs were green synthesized by adding the Astragalus sinicus extract to a copper sulfate solution. The acaricidal, larvicidal, and repellent activities of CuNPs against H. anatolicum were assessed via the adult immersion test, the larval packet test, and the vertical movement behavior of tick larvae, respectively. The effects of CuNPs on acetylcholinesterase as well as oxidative enzyme activities were examined. Results: The green synthesized CuNPs displayed a spherical form with a size range of 15-75 nm. After exposure of adult H. anatolicum to different concentrations of CuNPs, the viability rate of adult H. anatolicum and the mean number, weight, and hatchability of eggs were noticeably reduced, in comparison to the control group (P<0.001). In addition, the viability rate of larvae considerably declined (P<0.001) with the LC₅₀ and LC₉₀ values of 11.30 and 20.34 µg/mL, respectively. The maximum repellent activity of CuNPs was observed at 50, 100, and 200 µg/mL with complete repellent activity after 60, 120, and 180 min of exposure, respectively. CuNPs, mainly at ½LC₅₀ and LC₅₀ concentrations, markedly suppressed the acetylcholinesterase activity of the larval stage of H. anatolicum (P<0.001). Moreover, CuNPs, mainly at LC₅₀ dose, significantly elevated malondialdehyde level while declining glutathione-S-transferase level in H. anatolicum larvae (P<0.001). Conclusions: CuNPs show potent acaricidal, larvicidal, and repellent activities against adults and larvae of H. anatolicum. However, further studies must be performed to clarify the precise mechanisms and the efficacy of CuNPs in practical use.

Keywords

Insecticide / Tick / Pesticide / Astragalus sinicus / Nanomedicine / Hyalomma anatolicum / Acaricidal activity / Larvacidal activity

Acknowledgments

The authors would like to thank the Deanship of Scientific Research at Shaqra University and the Deanship of Scientific Research at Almaarefa University for supporting this work.

Conflict of interest statement

The authors declare that they have no competing interests.

Funding

The authors received no extramural funding for the study.

Authors’ contributions

HSG and ADA designed the work and contributed to the concept. BMA, AFS, MSA, and QAHM performed experiments. ADA and HSG wrote and performed the critical revision of the manuscript. All authors have read and agreed to the published version of the manuscript.

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