Modulatory effect of D-pinitol on bleomycin-induced pulmonary fibrosis in rats

Abstract

Objective: To assess the effect of D-pinitol on pulmonary fibrosis induced by bleomycin. Methods: Sprague-Dawley rats received intratracheal bleomycin (6 IU/kg) to induce pulmonary fibrosis, followed by administration of either D-pinitol (5, 10, or 20 mg/kg) or vehicle or methylprednisolone (10 mg/kg) over 28 days after bleomycin administration. Lung function, biochemical parameters, serum biochemistry, mRNA expressions, and histological features were observed. Results: D-pinitol at 10 and 20 mg/kg significantly (P<0.05) attenuated bleomycin-induced bronchoalveolar lavage fluid, decreased myeloperoxidase, nitric oxide, malondialdehyde levels, and increased glutathione and superoxide dismutase level. D-pinitol also improved lung function (enhanced pause, frequency of breathing, expired volume, and tidal volume). Besides, D-pinitol significantly (P<0.05) upregulated Nrf2 and downregulated mRNA expressions of TGF-β, collagen-1, and Smad-3. Furthermore, considerably less inflammation (peribronchial, perivascular, and total), Ashcroft, and interstitial fibrosis scores were observed in the D-pinitol group. Conclusions: D-pinitol exerts its effect against bleomycin-induced pulmonary fibrosis via antioxidative and anti-fibrotic pathways.

Keywords

Antioxidant / Bleomycin / Collagen-1 / D-pinitol / Pulmonary fibrosis / Smad-3 / TGF-β

Conflict of interest statement

The authors declare that they have no conflict of interest.

Funding

The authors received no extramural funding for this study.

Authors’ contributions

YLD: concept, design, literature search, data analysis, manuscript preparation, and manuscript review. ZHW: concept, data acquisition, manuscript editing and manuscript review. YXH: literature search, data analysis, manuscript editing and manuscript review. YZ: concept, design, data analysis, manuscript editing and manuscript review. XRW: concept, literature search, data acquisition, manuscript preparation, and manuscript review. CKG: concept, data acquisition, manuscript preparation, and manuscript review. LLB: concept, design, literature search, data acquisition, data analysis, manuscript preparation, manuscript editing and manuscript review.

Publisher’s note

The Publisher of the Journal remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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